<i>Escherichia coli</i>bind to urinary bladder epithelium through nonspecific sialic acid mediated adherence

Sakarya, Serhan; Ertem, Günay Tuncer; Öncü, Serkan; Koçak, İzzet; Erol, Nural; Oncu, Selcan

doi:10.1016/s0928-8244(03)00185-8

Cited by 21 publications

(14 citation statements)

References 48 publications

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“…This study shows, however, that N-acetylneuraminic acid on the surface of intestinal cells may be involved in CS21-mediated adherence to cells as neuraminic acid and neuraminidase pretreatment of cells before infection also abrogated adherence. Neuraminic acid derivatives, collectively known as sialic acids, are present as glycoconjugates in tissues of all mammals and bacterial cell surfaces and play an essential role in molecular recognition (Sakarya et al, 2003;Sohanpal et al, 2004). Glycan modifies pili expressed by Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

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Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

et al. 2013

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Colonization surface antigens (CSs) represent key virulence-associated factors of enterotoxigenic Escherichia coli (ETEC) strains. They are required for gut colonization, the first step of the diarrhoeal disease process induced by these bacteria. One of the most prevalent CSs is CS21, or longus, a type IV pili associated with bacterial self-aggregation, protection against environmental stresses, biofilm formation and adherence to epithelial cell lines. The objectives of this study were to assess the role of CS21 in adherence to primary intestinal epithelial cells and to determine if CS21 contributes to the pathogenesis of ETEC infection in vivo. We evaluated adherence of a CS21-expressing wild-type ETEC strain and an isogenic CS21-mutant strain to pig-derived intestinal cell lines. To determine the role of CS21 in pathogenesis we used the above ETEC strains in a neonatal mice challenge infection model to assess mortality. Quantitative adherence assays confirmed that ETEC adheres to primary intestinal epithelial cells lines in a CS21-dependent manner. In addition, the CS21-mediated ETEC adherence to cells was specific as purified LngA protein, the CS21 major subunit, competed for binding with the CS21-expressing ETEC while specific anti-LngA antibodies blocked adhesion to intestinal cells. Neonatal DBA/2 mice died after intra-stomach administration of CS21-expressing strains while lack of CS21 expression drastically reduced the virulence of the wild-type ETEC strain in this animal model. Collectively these results further support the role of CS21 during ETEC infection and add new evidence on its in vivo relevance in pathogenesis.

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Section: Discussionmentioning

confidence: 99%

“…proteins may promote specific interactions with Gramnegative micro-organism structures such as pili and nonpili adhesins (Sakarya et al, 2003(Sakarya et al, , 2010. Accordingly, sialic acid bound to specific membrane proteins on primary intestinal cell lines may result in specific binding to CS21.…”

Section: Discussionmentioning

confidence: 99%

Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

et al. 2013

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“…The assay employed in this study has been used to evaluate binding of a wide variety of human pathogens, such as Neisseria meningitidis, E. coli, and pseudamonads, with human BECs as the target tissue (26,29,31). More recently the assay has been adapted for use with epithelium obtained from tissue sites better suited for evaluating interactions between bacteria and mammalian cells obtained from the urinary bladder (24) or vaginal epithelium (2). The binding assay used to study these interactions has several practical and research design advantages, one of which is that it can be used to evaluate adhesin/receptor relationships in intact but nonviable cells.…”

Section: Discussionmentioning

confidence: 99%

A Second Aggregatibacter actinomycetemcomitans Autotransporter Adhesin Exhibits Specificity for Buccal Epithelial Cells in Humans and Old World Primates

et al. 2007

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Previous work showed that the Aggregatibacter actinomycetemcomitans adhesin Aae demonstrated species specificity and tissue tropism to buccal epithelial cells (BECs) derived from humans and Old World primates, but a second, lower-affinity adhesin was noted. This study was designed to determine if Omp100 (also known as ApiA), a surface-expressed A. actinomycetemcomitans adhesin, is that second adhesin and if so to investigate its tissue tropism and species specificity. A targeted mutagenesis protocol was used to construct an isogenic apiA mutant and an aae apiA double mutant with wild-type A. actinomycetemcomitans. In addition, Escherichia coli strain DH5␣ was used to express apiA to further assess binding parameters. Results indicated that the apiA mutant strain showed significantly less binding to BECs than its parent strain (P < 0.05). Further, binding mediated by ApiA was specific to BECs from humans and Old World primates, as seen in both wild-type A. actinomycetemcomitans and E. coli expressing ApiA (P < 0.05). Pretreatment of wild-type A. actinomycetemcomitans cells with anti-ApiA antiserum reduced binding in a dose-dependent manner. The aae apiA double mutant completely abrogated A. actinomycetemcomitans binding to both human and Old World primate BECs. Taken together, these studies indicate that ApiA and Aae, in concert, modulate binding of A. actinomycetemcomitans to human BECs. Since the BEC is a prominent reservoir for A. actinomycetemcomitans, identification of this second adhesin could lead to important therapeutic strategies.

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“…These affect almost 1 billion women each year. UPEC biofilms can form on the epithelium of the bladder and upper urinary tract (28), as well as intracellularly, evading host defenses (1,2) and resulting in a disease that is often chronic.…”

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confidence: 99%

Role of the rapA Gene in Controlling Antibiotic Resistance of Escherichia coli Biofilms

Lynch

Dixon

Benoit

et al. 2007

Antimicrob Agents Chemother

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By using a high-throughput screening method, a mutant of a uropathogenic Escherichia coli strain affected in the rapA gene was isolated. The mutant formed normal-architecture biofilms but showed decreased penicillin G resistance, although the mutation did not affect planktonic cell resistance. Transcriptome analysis showed that 22 genes were down-regulated in the mutant biofilm. One of these genes was yhcQ, which encodes a putative multidrug resistance pump. Mutants with mutations in this gene also formed biofilms with decreased resistance, although the effect was less pronounced than that of the rapA mutation. Thus, an additional mechanism(s) controlled by a rapA-regulated gene(s) was involved in wild-type biofilm resistance. The search for this mechanism was guided by the fact that another down-regulated gene in rapA biofilms, yeeZ, is suspected to be involved in extra cell wall-related functions. A comparison of the biofilm matrix of the wild-type and rapA strains revealed decreased polysaccharide quantities and coverage in the mutant biofilms. Furthermore, the (fluorescent) functional penicillin G homologue Bocillin FL penetrated the mutant biofilms more readily. The results strongly suggest a dual mechanism for the wild-type biofilm penicillin G resistance, retarded penetration, and effective efflux. The results of studies with an E. coli K-12 strain pointed to the same conclusion. Since efflux and penetration can be general resistance mechanisms, tests were conducted with other antibiotics. The rapA biofilm was also more sensitive to norfloxacin, chloramphenicol, and gentamicin.Bacterial biofilms are differentiated communities of sessile cells. Infections involving biofilms play a major role in such diverse medical conditions as endocarditis (24), cystic fibrosis (29), otitis media (17), and urinary tract infections (3), presenting a significant threat to human health (12, 16). Although this sessile mode of growth has long been known, the fact that it constitutes a normal part of the bacterial life cycle has been recognized only relatively recently. The medical importance of biofilms lies in the fact that in comparison to their free-living (planktonic) counterparts, biofilms possess increased resistance to antimicrobial agents (12, 16), and consequently, diseases in which biofilms have a dominant role tend to be chronic and difficult to eradicate.The focus of this study was uropathogenic Escherichia coli (UPEC), which is primarily responsible for urinary tract infections. These affect almost 1 billion women each year. UPEC biofilms can form on the epithelium of the bladder and upper urinary tract (28), as well as intracellularly, evading host defenses (1, 2) and resulting in a disease that is often chronic.Several genes play a role in biofilm formation (9,11,20), but whether the same genes are involved in the development of biofilm resistance has been relatively little studied. Mah et al. (21) identified nvdB gene mutants whose antibiotic resistance was not affected while they were in the planktonic stat...

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Escherichia colibind to urinary bladder epithelium through nonspecific sialic acid mediated adherence

Cited by 21 publications

References 48 publications

Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

A Second Aggregatibacter actinomycetemcomitans Autotransporter Adhesin Exhibits Specificity for Buccal Epithelial Cells in Humans and Old World Primates

Role of the rapA Gene in Controlling Antibiotic Resistance of Escherichia coli Biofilms

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