<i>Endozoicomonas</i> Dominates the Gill and Intestinal Content Microbiomes of <i>Mytilus edulis</i> from Barnegat Bay, New Jersey

Schill, William B.; Iwanowicz, Deborah D.; Adams, Cynthia R.

doi:10.2983/035.036.0212

Cited by 16 publications

(9 citation statements)

References 54 publications

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“…In this context, our values of the Simpson Index, Shannon Index, and observed OTUs were higher than those reported in samples of stomach and intestine of Mytilus edulis from Barnegat Bay (New Jersey, USA) [57]. Similarly, values of OTUs, Simpson Index, and Pielou's Evenness in intestine and stomach tissues of oysters from coastal waters of Louisiana (USA) were also lower than those observed in our study [58]. In contrast, OTU values of tissues in different oysters from the Gulf of California in Mexico (intestines) [59] and the Atlantic beach of North Carolina, USA (digestive glands) [60] were higher than the values obtained in this study.…”

Section: Discussioncontrasting

confidence: 75%

16S rRNA–Based Analysis Reveals Differences in the Bacterial Community Present in Tissues of Choromytilus chorus (Mytilidae, Bivalvia) Grown in an Estuary and a Bay in Southern Chile

et al. 2021

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Microbiota associated with bivalves have drawn considerable attention because studies have suggested their relevance to the fitness and growth of marine bivalves. Although the mussel Choromytilus chorus is a valuable resource for Chilean aquaculture and fisheries, its microbiota is still unknown. In this study, the composition and predicted functions of the bacterial community in tissues of C. chorus specimens grown in an estuary (Nehuentue) and a bay (Hueihue) were investigated. Using 16S rRNA genes as targets, the bacterial abundance in tissues was estimated by quantitative PCR and sequenced via Illumina MiSeq. The abundances of bacteria ranged from 103 to 105 copies of 16S rRNA genes g−1 tissue. In the Nehuentue estuary, the bacterial communities in the tissues were dominated by the Tenericutes phylum, whereas the Tenericutes and Proteobacteria phyla dominated in mussels from Hueihue Bay. Higher numbers of operational taxonomic units (OTUs) were observed in tissues from the Nehuentue Estuary than in those from Hueihue Bay. Differences in bacterial community compositions in tissues between both locations were confirmed by nonmetric multidimensional scaling (nMDS) and Venn diagram analysis. In addition, linear discriminant analysis effect size (LEfSe) revealed that the Mollicutes class and Actynomycetales order were key phylotypes in tissues from the Nehuentue Estuary and Hueihue Bay, respectively. Our analysis also predicted a high abundance of sequences assigned to heterotrophy; however, relatively high functional diversity was also found in tissues from Hueihue Bay. This work represents our first attempt to elucidate the C. chorus microbiota in contrasting Chilean aquatic environments.

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Section: Discussioncontrasting

confidence: 75%

16S rRNA–Based Analysis Reveals Differences in the Bacterial Community Present in Tissues of Choromytilus chorus (Mytilidae, Bivalvia) Grown in an Estuary and a Bay in Southern Chile

et al. 2021

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“…In fact, there is an apparent overall dominance of Endozoicomonadaceae in T. maxima , but we observed also distinct differences across tissues. High abundances of Endozoicomonadaceae have been previously also reported for tissues of other bivalves, such as intestines of the comb pen shell Atrina pectinate (Hyun et al, 2014), the gills of the deep-water bivalve Acesta excavate (Jensen et al, 2010), as well as both, gills and intestinal content of the blue mussel Mytilus edulis (Schill et al, 2017). For the latter, the authors also reported tissue-dependent abundances of Endozoicomonadaceae (sequences assessed from gills and intestinal content comprised up to 67.6 and 37.2%, respectively of one OTU representing this family).…”

Section: Discussionmentioning

confidence: 60%

Tissue-Specific Microbiomes of the Red Sea Giant Clam Tridacna maxima Highlight Differential Abundance of Endozoicomonadaceae

et al. 2019

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Giant clams (subfamily Tridacninae) are prevalent members of coral reef communities and engage in symbioses with algal photosymbionts of the family Symbiodiniaceae, similar to their scleractinian coral counterparts. However, we know little about their associated bacterial microbiome members. Here, we explored bacterial community diversity of digestive system, gill, and mantle tissues associated with the giant clam Tridacna maxima across a cross-shelf gradient (inshore, midshore, and offshore reef sites) in the central Red Sea using 16S rRNA gene amplicon sequencing. Different tissues harbor spatially stable and distinct microbial communities. Notably, diverse assemblages of bacteria affiliated to the family Endozoicomonadaceae were prevalent in all tissues, but particularly abundant in gills and to a lesser extent in digestive tissues. Besides Endozoicomonadaceae, bacteria in the families Pasteurellaceae, Alteromonadaceae, and Comamonadaceae were common associates, depending on the tissue queried. Taxonomy-based functional inference identified processes related to nitrogen cycling (among others) to be enriched in giant clam tissues and contributed by Endozoicomonadaceae. Our study highlights the tissue-specificity and broad taxonomic range of Endozoicomonadaceae associates, similar to other marine invertebrates, and suggests their contribution to nitrogen-related pathways. The investigation of bivalve-associated microbiome communities provides an important addition to the pathogen-focused studies for commercially important bivalves (e.g., oysters).

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“…In the 2008 samples, only one of the indicator ASVs was able to be classified past the level of domain, a member of the genus Endozoicomonas . This genus has been of much interest over the past several years due to its association with a wide array of marine hosts (Neave et al, 2017; Schill et al, 2017). Although this particular ASV was found in nearly every sample across the years, its massive proliferation in 2008 is notable and may be due to its role in carbon sugar transport and protein secretion, which may provide benefits to the host (Neave et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Decade‐scale stability and change in a marine bivalve microbiome

et al. 2021

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Predicting how populations and communities of organisms will respond to anthropogenic change is of paramount concern in ecology today. For communities of microorganisms, however, these predictions remain challenging, primarily due to data limitations. Information about long‐term dynamics of host‐associated microbial communities, in particular, is lacking. In this study, we use well‐preserved and freshly collected samples of soft tissue from a marine bivalve host, Donax gouldii, at a single site to quantify the diversity and composition of its microbiome over a decadal timescale. Site‐level measurements of temperature, salinity and chlorophyll a allowed us to test how the microbiome of this species responded to two natural experiments: a seasonal increase in temperature and a phytoplankton bloom. Our results show that ethanol‐preserved tissue can provide high‐resolution information about temporal trends in compositions of host‐associated microbial communities. Specifically, we found that the richness of amplicon sequence variants (ASVs) associated with D.gouldii did not change significantly over time despite increases in water temperature (+1.6°C due to seasonal change) and chlorophyll a concentration (more than ninefold). The phylogenetic composition of the communities, on the other hand, varied significantly between all collection years, with only six ASVs persisting over our sampling period. Overall, these results suggest that the diversity of microbial taxa associated with D.gouldii has remained stable over time and in response to seasonal environmental change over the course of more than a decade, but such stability is underlain by substantial turnover in the composition of the microbiome.

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Endozoicomonas Dominates the Gill and Intestinal Content Microbiomes of Mytilus edulis from Barnegat Bay, New Jersey

Cited by 16 publications

References 54 publications

16S rRNA–Based Analysis Reveals Differences in the Bacterial Community Present in Tissues of Choromytilus chorus (Mytilidae, Bivalvia) Grown in an Estuary and a Bay in Southern Chile

16S rRNA–Based Analysis Reveals Differences in the Bacterial Community Present in Tissues of Choromytilus chorus (Mytilidae, Bivalvia) Grown in an Estuary and a Bay in Southern Chile

Tissue-Specific Microbiomes of the Red Sea Giant Clam Tridacna maxima Highlight Differential Abundance of Endozoicomonadaceae

Decade‐scale stability and change in a marine bivalve microbiome

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