Eimeria tenella: in vitro development in irradiated bovine kidney cells

Abstract

The initial infection and first-generation development of Eimeria tenella was quantified using a cloned MDBK (Madin-Darby Bovine Kidney) cell line, irradiated with gamma radiation prior to infection, as the host cell. Irradiated cell cultures were found to be more susceptible to infection and had a greater capacity to support parasite development than non-irradiated cultures. It was suggested that the larger proportion of cells in the G2 phase of the cell cycle, the larger individual cell size and the inhibition of cell division in the irradiated cultures were all factors contributing to the increased susceptibility to infection and capacity to support parasite growth and development. The application of this technique (host cell irradiation) to the cultivation of other intracellular, protozoan parasites is discussed.

The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture

“…There are several immortalized cell lines that can support Eimeria sporozoite infection and first generation schizogony. In a comparative analysis Tierney and Mulcahy ( 2003 ) concluded that MDBK cells were most suitable, and this has been the line of choice for research in E. tenella for many years (Crane et al, 1984 ). Using this in vitro MDBK system we observed fully formed first-generation merozoites that exhibit the same structures as those described previously for first-generation merozoites generated in chick kidney cells (Pacheco et al, 1975 ).…”

Eimeria tenella: quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites

“…tenella oocysts (Merck strain LS-18) maintained by passage in chickens were used as the source of E. tenella sporozoites. Purified, sterile oocysts and purified sporozoites were obtained as described previously (Schmatz, Crane & Murray 1984). The vertebrate host cell used for in uitro studies was a clone (MDBK/44 1) of Madin-Darby bovine kidney cells (American Type Culture Collection CCL22).…”

“…The vertebrate host cell used for in uitro studies was a clone (MDBK/44 1) of Madin-Darby bovine kidney cells (American Type Culture Collection CCL22). Stock cultures were grown and maintained at 41°C as described previously (Crane et al 1984) with the exception that lactalbumin hydrolysate was omitted from the culture medium. Chickens used as hosts for in viuo studies were 2-3 week old Hubbard White Leghorns (Avian Services, Frenchtown, NJ).…”

Dual RNA-Seq transcriptome analysis of chicken macrophage-like cells (HD11) infected in vitro with Eimeria tenella

“…We wanted to explore this approach to obtain a comprehensive view of parasite and host events during the early phase of Eimeria infection of chicken cells in vitro. Eimeria parasites are not readily propagated to perform their full life cycle in cell-line culture but cell-line systems where the first generation schizogony of E. tenella takes place have been described (Patton, 1965;Crane et al, 1984;Heriveau et al, 2000;Tierney and Mulcahy, 2003;Bussière et al, 2018). Eimeria tenella is one of the most pathogenic Eimeria species that infects chickens and it replicates exclusively in the chicken caecal tissues (Chapman and Shirley, 2003).…”