EGFRmutant-specific immunohistochemistry has high specificity and sensitivity for detecting targeted activatingEGFRmutations in lung adenocarcinoma

Abstract

Mutant-specific EGFR IHC has good specificity and sensitivity for identifying targeted activating EGFR mutations. Although inferior to molecular genetic analysis of the EGFR gene, IHC is highly specific and sensitive for the targeted EGFR mutations. The antibodies are likely to be of clinical value in cases where limited tumour material is available, or in situations where molecular genetic analysis is not readily available.

Epidermal growth factor receptor mutation‐specific immunohistochemical antibodies in lung adenocarcinoma

“…The sensitivity and specificity of the previously reported antibodies (clones 43B2 and 6B6) has varied in different studies with varying study populations (Supporting information, Table S8) . Nevertheless, the performance of these antibodies in this study resembled that in the previous reports (Supporting information, Figure S1).…”

Overexpression of EGFR Protein in Bruneian Lung Cancer Patients

“…Patients with squamous carcinoma, large cell carcinoma, malignant lymphoma and carcinoid tumour tend to show a greater amount of EGFR protein expression. In principal, our finding is consistent with previously published work (Cooper et al, 2013) In addition, our data showed that the EGFR protein expression does not correlate with the clinicopathologic variables such as sex (p=0.004), age (p=0.02) and race (p=0.008). These findings are in agreement with previous findings (Li et al, 2011).…”

“…The reason for using Immunohistochemistry (IHC), being IHC is one of the most commonly used methods of evaluating EGFR levels (Cooper et al, 2013). Other techniques such as immunoassay, enzyme-linked immunosorbent assay (ELISA) and fluorescence in-situ hybridization (FISH) are also used (Pauletti et al, 2000;Salam et al, 2009) However, due to IHC being relatively simple to perform, and easily replicable in almost any laboratory, it has potential to be a screening test for identifying molecular biomarkers for lung cancer (Grands et al, 1996).…”

Detection of HPV subtypes by mass spectrometry in FFPE tissue specimens: a reliable tool for routine diagnostics

“…MS as a method to detect PCR amplification products is well established and has gained great acceptance for the identifications of genetic mutations 16. Flexible testing for additional PCR products is possible, and hands-on time is generally comparable to the chip-based assays and reduced compared with sequencing analyses 11.…”