Echinococcus granulosus: a comparison of free amino acid concentration in hydatid fluid from primary and secondary cysts and host plasma

Abstract

A total of 28 components were detected in the free amino acid (FAA) pool of hydatid fluid from primary and secondary equine cysts, secondary ovine cysts and host plasma. Examination of data from equine cysts revealed that the majority of FAAs were present in significantly greater concentrations in secondary cysts, glycine being over 30 times more concentrated. Values for total carbohydrates and glucose did not, however, differ significantly and total protein content was greater in primary cysts. Comparison of the (FAA) pool of secondary equine and ovine cysts revealed strain variation. It was demonstrated that most FAAs were more concentrated in hydatid fluid than in the corresponding host plasma, many concentration ratios exceeding 10. The possible contribution that mediated amino acid transport across the cyst wall and parasite amino acid metabolism makes to the composition of the FAA pool was discussed. No significant plasma aminoacidaemia was associated with infection.

In vitro metabolomic footprint of the Echinococcus multilocularis metacestode

“…Myo-inositol was detected in traces in VF, not in vcDMEM, but no further focus was laid on this metabolite in the present study. Overall, our results on VF composition are in agreement with previous studies on the composition of E. granulosus ex vivo hydatid fluid 34,37,38,41 . The only difference is that in E. multilocularis metacestode VF, alanine is the most abundant amino acid whereas in hydatid fluid, glycine is the most abundant amino acid 34,41,69 .…”

Oxfendazole Treatment for Cystic Hydatid Disease in Naturally Infected Animals

“…The cyst is filled with hypotonic fluid containing both host and parasite proteins. The cyst wall, therefore, must be capable of active transport and selective absorption and excretion (11). Drugs may have to penetrate all three layers of the cyst in order to kill the living parasite tissues.…”

Metabolite pattern ofCysticercus cellulosaemetacestode from different predilection sites of swine using proton magnetic resonance spectroscopy

“…In India, the prevalence of cysticercosis in swine has been reported to be 18 % (Pathak et al, 1983) and high proportion of pork is rejected due to this infection (Pathak & Gaur, 1988, 1989, In rural areas, where meat inspection is usually not performed, the number of animals affected by the disease may be much higher. This is due to the fact that swine walk about freely, feeding intentionally or unintentionally on human faecal matter, which may contain ova or gravid proglottids of T. solium.…”