E. colicells advance into phase-separated (biofilm-simulating) extracellular polymeric substance containing DNA, HU, and lipopolysaccharide
Archit Gupta,
Purnananda Guptasarma
Abstract:We have earlier shown that HU, a nucleoid-associated protein, uses its DNA-binding surfaces to bind to bacterial outer-membrane lipopolysachharide (LPS), with this explaining how HU act as a potential glue for the adherence of bacteria to DNA, e.g., in biofilms. We have also earlier shown that HU and DNA together condense into a state of liquid-liquid phase separation (LLPS) both within, and outside, bacterial cells. Here, we report that HU and free LPS also condense into a state of phase separation, with coac… Show more
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