<i>Drosophila melanogaster</i>establishes a species-specific mutualistic interaction with stable gut-colonizing bacteria

Pais, Inês S.; Valente, Rita S; Sporniak, Marta; Teixeira, Luı́s

doi:10.1101/265991

Cited by 18 publications

(13 citation statements)

References 114 publications

(160 reference statements)

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“…A total of 2 672 402 Wolbachia sequences were removed. We grouped the sequences into 100% identity operational taxonomic units (OTUs) to resolve strain‐level differences because the interaction with the fly host may differ for closely related bacteria (Ryu et al ., ; Pais et al ., ). Composition and diversity of the bacterial microbiome were comparable to those from previous studies (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Conversely, we expected that Acetobacteraceae, in general, would be enriched in flies over substrate because this family contains several members that benefit D . melanogaster (Shin et al ., ; Pais et al ., ). Our analysis confirmed this expectation (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Pais and colleagues. () showed that Acetobacter thailandicus can persist in the gut of D . melanogaster , can be dispersed by the host and provide a fitness benefit to the host, while closely related Acetobacter strains cannot.…”

Section: Introductionmentioning

confidence: 99%

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Common structuring principles of the Drosophila melanogaster microbiome on a continental scale and between host and substrate

Wang

Kapun

Waidele

et al. 2020

Environ Microbiol Rep

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Summary The relative importance of host control, environmental effects and stochasticity in the assemblage of host‐associated microbiomes is being debated. We analysed the microbiome among fly populations that were sampled across Europe by the European Drosophila Population Genomics Consortium (DrosEU). In order to better understand the structuring principles of the natural D. melanogaster microbiome, we combined environmental data on climate and food‐substrate with dense genomic data on host populations and microbiome profiling. Food‐substrate, temperature, and host population structure correlated with microbiome structure. Microbes, whose abundance was co‐structured with host populations, also differed in abundance between flies and their substrate in an independent survey. This finding suggests common, host‐related structuring principles of the microbiome on different spatial scales.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

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Common structuring principles of the Drosophila melanogaster microbiome on a continental scale and between host and substrate

Wang

Kapun

Waidele

et al. 2020

Environ Microbiol Rep

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“…The environmental characters responsible for the variation could include temperature or diet (Chandler et al, ; Moghadam et al, ; Staubach et al, ), and at least some genetic factors that shape the microbiota composition of D. melanogaster have been described (Broderick, Buchon, & Lemaitre, ; Dobson et al, ). An additional or alternative explanation is that the characteristics described for laboratory flies may not reflect the biology of wild flies, since the interactions of Drosophila and their microbiota can vary depending if the partners are from the wild or the laboratory (Blum, Fischer, Miles, & Handelsman, ; Gould et al, ; Inamine et al, ; Obadia et al, ; Pais, Valente, Sporniak, & Teixeira, ; Winans et al, ). Therefore, we cannot rule out that characteristics defining interactions between laboratory Drosophila and their microbiota, such as inconstancy, are different for laboratory and wild flies.…”

Section: Discussionmentioning

confidence: 99%

The microbiota influences the Drosophila melanogaster life history strategy

et al. 2020

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Organisms are locally adapted when members of a population have a fitness advantage in one location relative to conspecifics in other geographies. For example, across latitudinal gradients, some organisms may trade off between traits that maximize fitness components in one, but not both, of somatic maintenance or reproductive output. Latitudinal gradients in life history strategies are traditionally attributed to environmental selection on an animal's genotype, without any consideration of the possible impact of associated microorganisms (“microbiota”) on life history traits. Here, we show in Drosophila melanogaster, a key model for studying local adaptation and life history strategy, that excluding the microbiota from definitions of local adaptation is a major shortfall. First, we reveal that an isogenic fly line reared with different bacteria varies the investment in early reproduction versus somatic maintenance. Next, we show that in wild fruit flies, the abundance of these same bacteria was correlated with the latitude and life history strategy of the flies, suggesting geographic specificity of the microbiota composition. Variation in microbiota composition of locally adapted D. melanogaster could be attributed to both the wild environment and host genetic selection. Finally, by eliminating or manipulating the microbiota of fly lines collected across a latitudinal gradient, we reveal that host genotype contributes to latitude‐specific life history traits independent of the microbiota and that variation in the microbiota can suppress or reverse the differences between locally adapted fly lines. Together, these findings establish the microbiota composition of a model animal as an essential consideration in local adaptation.

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“…Findings from recent studies of the ecology of intestinal microbes of both Drosophila and mammals point out the importance of the innate immune response in maintaining the integrity of the host intestinal epithelium when confronted with microbial antigens. In addition to the abundant transient bacteria that constantly pass through their guts, symbiotic bacteria have established stable colonies in the guts of their animal hosts (Pais, Valente, Spomiak, & Teixeira, ). Cellular immune responses mediate the interaction of microbes and host cells and control colonization by symbiotic microbes.…”

Section: Introductionmentioning

confidence: 99%

Interfaces between microbes and membranes of host epithelial cells in hemipteran midguts

Nardi

Miller

Bee

2019

Journal of Morphology

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Certain families of plant‐feeding insects in the order Hemiptera (infraorder Pentatomomorpha) have established symbiotic relationships with microbes that inhabit specific pouches (caeca) of their midgut epithelium. The placement of these caeca in a well‐delineated region at the most posterior end of the midgut bordering the hindgut is conserved in these families; in situ the convoluted midgut is predictably folded so that this caecal region lies adjacent to the anterior‐most region of the midgut. Depending on the hemipteran family, caeca vary in their number and configuration at a given anterior–posterior location. At the host‐microbe interface, epithelial plasma membranes of midgut epithelial cells interact with nonself antigens of microbial surfaces. In the different hemipteran species examined, a continuum of interactions is observed between microbes and host membranes. Bacteria can exist as free living cells within the midgut lumen without contacting host membranes while other host cells physically interact extensively with microbial surfaces by extending numerous processes that interdigitate with microbes; and, in many instances, processes completely envelope the microbes. The host cells can embrace the foreign microbes, completely enveloping each with a single host membrane or sometimes enveloping each with the two additional host membranes of a phagosome.

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Drosophila melanogasterestablishes a species-specific mutualistic interaction with stable gut-colonizing bacteria

Abstract: 16Animals live together with diverse bacteria that can impact their biology. In Drosophila

Cited by 18 publications

References 114 publications

Common structuring principles of the Drosophila melanogaster microbiome on a continental scale and between host and substrate

Common structuring principles of the Drosophila melanogaster microbiome on a continental scale and between host and substrate

The microbiota influences the Drosophila melanogaster life history strategy

Interfaces between microbes and membranes of host epithelial cells in hemipteran midguts

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