<i>Drosophila</i>
            Mitotic Domain Boundaries as Cell Fate Boundaries

Cambridge, Sidney B.; Davis, Robert L.; Minden, Jonathan S.

doi:10.1126/science.277.5327.825

Cited by 61 publications

(37 citation statements)

References 20 publications

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“…Likewise, a caged, inactive version of GAL4-VP16 can be injected into embryos and activated by a beam of UV (365 nm) light, thereby controlling responder expression in a highly specific fashion (Cambridge et al, 1997). Although these latter techniques provide a useful approach to directing desired embryonic GAL4 patterns, their value in addressing later stages of development is limited.…”

Section: The Corkscrew: Increased Inducibilitymentioning

confidence: 99%

GAL4 system in drosophila: A fly geneticist's swiss army knife

Duffy

2002

Genesis

967

759

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Section: The Corkscrew: Increased Inducibilitymentioning

confidence: 99%

GAL4 system in drosophila: A fly geneticist's swiss army knife

Duffy

2002

Genesis

967

759

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“…To circumvent this problem, several alternate approaches have been proposed. For instance, cell-specific activation of hs transgenes was achieved with a laser microbeam (11), and light-induced activation of caged GAL4 was used to activate UAS transgenes in individual embryonic cells (12). However, these approaches are technically demanding and are restricted to certain developmental stages.…”

mentioning

confidence: 99%

A conditional tissue-specific transgene expression system using inducible GAL4

Osterwalder

Yoon²,

White³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

711

756

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In Drosophila, the most widely used system for generating spatially restricted transgene expression is based on the yeast GAL4 protein and its target upstream activating sequence (UAS). To permit temporal as well as spatial control over UAS-transgene expression, we have explored the use of a conditional RU486-dependent GAL4 protein (GeneSwitch) in Drosophila. By using cloned promoter fragments of the embryonic lethal abnormal vision gene or the myosin heavy chain gene, we have expressed GeneSwitch specifically in neurons or muscles and show that its transcriptional activity within the target tissues depends on the presence of the activator RU486 (mifepristone). We used available UAS-reporter lines to demonstrate RU486-dependent tissuespecific transgene expression in larvae. Reporter protein expression could be detected 5 h after systemic application of RU486 by either feeding or ''larval bathing.'' Transgene expression levels were dose-dependent on RU486 concentration in larval food, with low background expression in the absence of RU486. By using genetically altered ion channels as reporters, we were able to change the physiological properties of larval bodywall muscles in an RU486-dependent fashion. We demonstrate here the applicability of GeneSwitch for conditional tissue-specific expression in Drosophila, and we provide tools to control pre-and postsynaptic expression of transgenes at the larval neuromuscular junction during postembryonic life.

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“…Although this finding was not tested in the endoderm, it is possible that cells within this tissue also respect compartment boundaries. In Drosophila embryos, mitotic domain boundaries can define cell fate boundaries (Cambridge et al, 1997). Because in the gut, the proliferating stem cell compartment is not anchored in the crypts until after birth, it is possible that final epithelial pyloric border formation requires establishment of the differential domains of mitotic activity (very rapid in the intestine and slower in gastric tissue).…”

Section: Discussionmentioning

confidence: 99%

Villin: A marker for development of the epithelial pyloric border

Braunstein

Qiao

Madison

et al. 2002

Developmental Dynamics

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In the adult gastrointestinal tract, the morphologic borders between esophagus and stomach and between stomach and small intestine are literally one cell thick. The patterning mechanisms that underlie the development of these sharp regional divisions from a once continuous endodermal tube are still obscure. In the embryonic endoderm of the developing gut, region-specific expression of certain genes (e.g., intestine-specific expression of the actin bundling protein villin) can be detected as early as 9.0 days post coitum, although the morphologic differentiation of the gut epithelium proper does not begin until 4 to 5 days later. By using a mouse model in which a ␤-galactosidase marker has been inserted into the endogenous villin locus, we examined the development of the stomach/ intestinal (pyloric) border during gut organogenesis. The data indicate that the border is not sharp from the outset. Rather, the initial border region is characterized by a decreasing gradient of villin/␤-galactosidase expression that extends into the distal stomach. A sharp epithelial border of villin/␤-galactosidase expression appears abruptly at day 16 and is further refined over the next 3 weeks to form the distinct onecell-thick border characteristic of the adult. These results indicate that an important previously unrecognized patterning event occurs in the gut epithelium at 16 days; this event may define an epithelial compartment boundary between the stomach and the intestine. The villin/ ␤-galactosidase mouse model characterized here provides an excellent substrate with which to further dissect the mechanisms involved in this patterning process.

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Drosophila Mitotic Domain Boundaries as Cell Fate Boundaries

Cited by 61 publications

References 20 publications

GAL4 system in drosophila: A fly geneticist's swiss army knife

GAL4 system in drosophila: A fly geneticist's swiss army knife

A conditional tissue-specific transgene expression system using inducible GAL4

Villin: A marker for development of the epithelial pyloric border

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