<i>Drosophila dMRP4</i>regulates responsiveness to O<sub>2</sub>deprivation and development under hypoxia

Huang, He; Haddad, Gabriel G.

doi:10.1152/physiolgenomics.00166.2006

Cited by 19 publications

(11 citation statements)

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“…Real-time PCR results showed that Mi{ET1}Mdr49 has about two fold higher Mdr49 expression than controls even after 10 days of IH exposure (P<0.05, t-test, Figure 3D), suggesting that sustained increased expression of Mdr49 is beneficial in survival over longer periods of IH treatment. In order to reinforce our hypothesis that Multi-drug resistance/transport is linked to IH tolerance, we tested CG14709 gene which belongs to the same Multi-drug resistance protein family [22]. We over-expressed this gene ubiquitously using da-Gal4 driver and confirmed that the F1 progeny had >10 fold higher mRNA level than controls (Figure 3D).…”

Section: Resultsmentioning

confidence: 66%

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

et al. 2009

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BackgroundConstant hypoxia (CH) and intermittent hypoxia (IH) occur during several pathological conditions such as asthma and obstructive sleep apnea. Our research is focused on understanding the molecular mechanisms that lead to injury or adaptation to hypoxic stress using Drosophila as a model system. Our current genome-wide study is designed to investigate gene expression changes and identify protective mechanism(s) in D. melanogaster after exposure to severe (1% O2) intermittent or constant hypoxia.Methodology/Principal FindingsOur microarray analysis has identified multiple gene families that are up- or down-regulated in response to acute CH or IH. We observed distinct responses to IH and CH in gene expression that varied in the number of genes and type of gene families. We then studied the role of candidate genes (up-or down-regulated) in hypoxia tolerance (adult survival) for longer periods (CH-7 days, IH-10 days) under severe CH or IH. Heat shock proteins up-regulation (specifically Hsp23 and Hsp70) led to a significant increase in adult survival (as compared to controls) of P-element lines during CH. In contrast, during IH treatment the up-regulation of Mdr49 and l(2)08717 genes (P-element lines) provided survival advantage over controls. This suggests that the increased transcript levels following treatment with either paradigm play an important role in tolerance to severe hypoxia. Furthermore, by over-expressing Hsp70 in specific tissues, we found that up-regulation of Hsp70 in heart and brain play critical role in tolerance to CH in flies.Conclusions/SignificanceWe observed that the gene expression response to IH or CH is specific and paradigm-dependent. We have identified several genes Hsp23, Hsp70, CG1600, l(2)08717 and Mdr49 that play an important role in hypoxia tolerance whether it is in CH or IH. These data provide further clues about the mechanisms by which IH or CH lead to cell injury and morbidity or adaptation and survival.

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Section: Resultsmentioning

confidence: 66%

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

et al. 2009

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“…The ABCC transporter encoded by CG10505 in D. melanogaster was regulated by heavy metals and was involved in biochemical detoxification of zinc and copper [45]. Drosophila DmCG14709 gene, a homologue of H. sapiens MRP4/ABCC4, was tightly regulated by oxygen [46]. The human body louse ABCC4 gene, PhABCC4 , was up-regulated after exposed to the pesticide ivermectin.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of ATP-binding cassette (ABC) transporters in the sweetpotato whitefly, Bemisia tabaci

Liu

Song

et al. 2017

BMC Genomics

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BackgroundABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants.ResultsA total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 23 ABCGs and 9 ABCHs. In comparison to other species, subfamilies G and H in both phloem- and blood-sucking arthropods are expanded. The temporal expression profiles of these 55 ABC transporters throughout B. tabaci developmental stages and their responses to imidacloprid, a neonicotinoid insecticide, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of 24 ABC transporters (44% of the total) representing all eight subfamilies was confirmed by the quantitative real-time PCR (RT-qPCR). Furthermore, mRNA expression levels estimated by RT-qPCR and RNA-seq analyses were significantly correlated (r = 0.684, p < 0.01).ConclusionsIt is the first genome-wide analysis of the entire repertoire of ABC transporters in B. tabaci. The identification of these ABC transporters, their temporal expression profiles during B. tabaci development, and their response to a neonicotinoid insecticide lay the foundation for functional genomic understanding of their contribution to the invasiveness of B. tabaci. Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3706-6) contains supplementary material, which is available to authorized users.

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“…D . melanogaster CG14709 controls responsiveness to O 2 deprivation and might also be involved in oxidative stress response [81,82] while 31% of embryos of D . melanogaster CG7627 mutants were unable to heal wounds 16 h postwounding [83].…”

Section: Resultsmentioning

confidence: 99%

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

et al. 2013

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BackgroundThe ABC (ATP-binding cassette) gene superfamily is widespread across all living species. The majority of ABC genes encode ABC transporters, which are membrane-spanning proteins capable of transferring substrates across biological membranes by hydrolyzing ATP. Although ABC transporters have often been associated with resistance to drugs and toxic compounds, within the Arthropoda ABC gene families have only been characterized in detail in several insects and a crustacean. In this study, we report a genome-wide survey and expression analysis of the ABC gene superfamily in the spider mite, Tetranychus urticae, a chelicerate ~ 450 million years diverged from other Arthropod lineages. T. urticae is a major agricultural pest, and is among of the most polyphagous arthropod herbivores known. The species resists a staggering array of toxic plant secondary metabolites, and has developed resistance to all major classes of pesticides in use for its control.ResultsWe identified 103 ABC genes in the T. urticae genome, the highest number discovered in a metazoan species to date. Within the T. urticae ABC gene set, all members of the eight currently described subfamilies (A to H) were detected. A phylogenetic analysis revealed that the high number of ABC genes in T. urticae is due primarily to lineage-specific expansions of ABC genes within the ABCC, ABCG and ABCH subfamilies. In particular, the ABCC subfamily harbors the highest number of T. urticae ABC genes (39). In a comparative genomic analysis, we found clear orthologous relationships between a subset of T. urticae ABC proteins and ABC proteins in both vertebrates and invertebrates known to be involved in fundamental cellular processes. These included members of the ABCB-half transporters, and the ABCD, ABCE and ABCF families. Furthermore, one-to-one orthologues could be distinguished between T. urticae proteins and human ABCC10, ABCG5 and ABCG8, the Drosophila melanogaster sulfonylurea receptor and ecdysone-regulated transporter E23. Finally, expression profiling revealed that ABC genes in the ABCC, ABCG ABCH subfamilies were differentially expressed in multi-pesticide resistant mite strains and/or in mites transferred to challenging (toxic) host plants.ConclusionsIn this study we present the first comprehensive analysis of ABC genes in a polyphagous arthropod herbivore. We demonstrate that the broad plant host range and high levels of pesticide resistance in T. urticae are associated with lineage-specific expansions of ABC genes, many of which respond transcriptionally to xenobiotic exposure. This ABC catalogue will serve as a basis for future biochemical and toxicological studies. Obtaining functional evidence that these ABC subfamilies contribute to xenobiotic tolerance should be the priority of future research.

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Drosophila dMRP4regulates responsiveness to O₂deprivation and development under hypoxia

Abstract: Huang H, Haddad GG. Drosophila dMRP4 regulates responsiveness to O 2 deprivation and development under hypoxia. Physiol

Cited by 19 publications

References 46 publications

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

Genome-wide analysis of ATP-binding cassette (ABC) transporters in the sweetpotato whitefly, Bemisia tabaci

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

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Drosophila dMRP4regulates responsiveness to O2deprivation and development under hypoxia

Abstract: Huang H, Haddad GG. Drosophila dMRP4 regulates responsiveness to O 2 deprivation and development under hypoxia. Physiol

Cited by 19 publications

References 46 publications

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster

Genome-wide analysis of ATP-binding cassette (ABC) transporters in the sweetpotato whitefly, Bemisia tabaci

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

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Drosophila dMRP4regulates responsiveness to O₂deprivation and development under hypoxia