<i>DREB</i>Genes from Common Bean (<i>Phaseolus vulgaris</i>L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity

Konzen, Enéas Ricardo; Recchia, Gustavo Henrique; Cassieri, Fernanda; Caldas, Danielle Gregorio Gomes; Teran, Jorge C. Berny Mier y; Gepts, Paul; Tsai, Siu Mui

doi:10.1155/2019/9520642

Cited by 22 publications

(18 citation statements)

References 86 publications

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“…We identified Lima bean orthologs of 30 genes having reported associations with traits in previous studies, 27 of which were reported in common bean. Traits included due to their importance in plant breeding are yield 44 , nutritional quality 45 , herbicide resistance 46 , plant architecture 47 , tolerance to abiotic stresses 48 , among others. Moreover, seed coat color and growth habit, important characteristics needed to meet consumer and farmer preferences in Lima bean and common bean breeding and marketing, were also included.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

et al. 2021

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Lima bean (Phaseolus lunatus L.), one of the five domesticated Phaseolus bean crops, shows a wide range of ecological adaptations along its distribution range from Mexico to Argentina. These adaptations make it a promising crop for improving food security under predicted scenarios of climate change in Latin America and elsewhere. In this work, we combine long and short read sequencing technologies with a dense genetic map from a biparental population to obtain the chromosome-level genome assembly for Lima bean. Annotation of 28,326 gene models show high diversity among 1917 genes with conserved domains related to disease resistance. Structural comparison across 22,180 orthologs with common bean reveals high genome synteny and five large intrachromosomal rearrangements. Population genomic analyses show that wild Lima bean is organized into six clusters with mostly non-overlapping distributions and that Mesomerican landraces can be further subdivided into three subclusters. RNA-seq data reveal 4275 differentially expressed genes, which can be related to pod dehiscence and seed development. We expect the resources presented here to serve as a solid basis to achieve a comprehensive view of the degree of convergent evolution of Phaseolus species under domestication and provide tools and information for breeding for climate change resiliency.

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Section: Resultsmentioning

confidence: 99%

Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

et al. 2021

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“…Among of which, the expression of PvDREB1F and PvDREB5A related by drought, salt, cold, and ABA; PvDREB2A and PvDREB6B were induced under dehydration and cold conditions. The study aided the understanding of the molecular mechanisms associated with drought, salt, and cold tolerance in common bean 16 .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the GmDREB6 gene enhances proline accumulation and salt tolerance in genetically modified soybean plants

Nguyen

et al. 2019

Sci Rep

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Soybean plants are sensitive to the effects of abiotic stress and belong to the group of crops that are less drought and salt tolerant. The identification of genes involved in mechanisms targeted to cope with water shortage is an essential and indispensable task for improving the drought and salt tolerance of soybean. One of the approaches for obtaining lines with increased tolerance is genetic modification. The dehydration-responsive element binding proteins (DREBs), belonging to the AP2 family, are trans-active transcription factors that bind to the cis-sequences of the promoter for activating the expression of the target genes that mediate drought and salt tolerant responses. In this study, the GmDREB6 transgene was introduced into DT84 cultivar soybean plants, using Agrobacterium-mediated transformation. The efficacy of GmDREB6 overexpression in enhancing the transcriptional level of GmP5CS and proline accumulation in genetically modified (GM) soybean plants was also assayed. The results demonstrated that ten GM soybean plants (T0 generation) were successfully generated from the transformed explants after selecting with kanamycin. Among these plantlets, the presence of the GmDREB6 transgene was confirmed in nine plants by Polymerase Chain Reaction (PCR), and eight plants showed positive results in Southern blot. In the T1 generation, four GM lines, labelled T1-2, T1-4, T1-7, and T1-10, expressed the recombinant GmDREB6 protein. In the T2 generation, the transcriptional levels of the GmP5CS gene were higher in the GM lines than in the non-transgenic plants, under normal conditions and also under conditions of salt stress and drought, ranging from 1.36 to 2.01 folds and 1.58 to 3.16 folds that of the non-transgenic plants, respectively. The proline content was higher in the four GM soybean lines, T2-2, T2-4, T2-7, and T2-10 than in the non-transgenic plants, ranging from 0.82 μmol/g to 4.03 μmol/g. The proline content was the highest in the GM T2-7 line (7.77 μmol/g). In GM soybean lines, T2-2, T2-4, T2-7, and T2-10 proline content increased after plants were subjected to salt stress for seven days, in comparison to that under normal conditions, and ranged from 247.83% to 300%, while that of the non-GM plants was 238.22%. These results suggested that GmDREB6 could act as a potential candidate for genetic engineering for improving tolerance to salt stresses.

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“…An alternative approach is to map genes with a putative role in drought-tolerance and examine to what extent they co-segregate with QTLs. Genome-wide categorizations have been published for a few families in common bean, such as aquaporins (AQP) (Ariani and Gepts, 2015) and Dehydration Responsive Element-Binding (DREB) genes (Cortés et al, 2012;Konzen et al, 2019). AQPs play important roles as water channel proteins in plants.…”

Section: Putative Candidate Genes For Qtlsmentioning

confidence: 99%

“…Phvul.001G136100 is located within the QTL at Pv01.45, a QTL detected in the 2015 well-watered treatment and Phvul.003G241700 is located at Pv03.72, a QTL detected in the well-watered treatment in 2015. Both genes were previously categorized as DREB2 genes, which are normally involved in responses to abiotic stresses such as caused by water deficit (Konzen et al, 2019).…”

Section: Putative Candidate Genes For Qtlsmentioning

confidence: 99%

Exploration of the Yield Potential of Mesoamerican Wild Common Beans From Contrasting Eco-Geographic Regions by Nested Recombinant Inbred Populations

et al. 2020

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Genetic analyses and utilization of wild genetic variation for crop improvement in common bean (Phaseolus vulgaris L.) have been hampered by yield evaluation difficulties, identification of advantageous variation, and linkage drag. The lack of adaptation to cultivation conditions and the existence of highly structured populations make association mapping of diversity panels not optimal. Joint linkage mapping of nested populations avoids the later constraint, while populations crossed with a common domesticated parent allow the evaluation of wild variation within a more adapted background. Three domesticated by wild backcrossed-inbred-line populations (BC 1 S 4) were developed using three wild accessions representing the full range of rainfall of the Mesoamerican wild bean distribution crossed to the elite drought tolerant domesticated parent SEA 5. These populations were evaluated under field conditions in three environments, two fully irrigated trials in two seasons and a simulated terminal drought in the second season. The goal was to test if these populations responded differently to drought stress and contained progenies with higher yield than SEA 5, not only under drought but also under water-watered conditions. Results revealed that the two populations derived from wild parents of the lower rainfall regions produced lines with higher yield compared to the domesticated parent in the three environments, i.e., both in the drought-stressed environment and in the well-watered treatments. Several progeny lines produced yields, which on average over the three environments were 20% higher than the SEA 5 yield. Twenty QTLs for yield were identified in 13 unique regions on eight of the 11 chromosomes of common bean. Five of these regions showed at least one wild allele that increased yield over the domesticated parent. The variation explained by these QTLs ranged from 0.6 to 5.4% of the total variation and the additive effects

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DREBGenes from Common Bean (Phaseolus vulgarisL.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity

Cited by 22 publications

References 86 publications

Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

Overexpression of the GmDREB6 gene enhances proline accumulation and salt tolerance in genetically modified soybean plants

Exploration of the Yield Potential of Mesoamerican Wild Common Beans From Contrasting Eco-Geographic Regions by Nested Recombinant Inbred Populations

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