<i>Didymellaceae</i> revisited

Chen, Q.; Hou, L.W.; Duan, Weijun; Crous, Pedro W.; Cai, Lei

doi:10.1016/j.simyco.2017.06.002

Cited by 200 publications

(215 citation statements)

References 36 publications

(81 reference statements)

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“…A part of the β-tubulin (BenA) and/or calmodulin (CaM) gene was amplified and sequenced for the isolates belonging to Aspergillus, Penicillium and Talaromyces as previously described (Houbraken et al, , 2012Samson et al, 2019). For other isolates, the ITS regions, a part of the translation elongation factor 1 alpha (TEF-1α) and/or the RNA polymerase II subunit (RPB2) gene were amplified and sequenced (Groenewald et al, 2005(Groenewald et al, , 2013Chen et al, 2015Chen et al, , 2017. Species identities were confirmed by comparing the generated sequences with sequences housed in the NCBI database and the internal curated database of the Westerdijk Fungal Biodiversity Institute (WI).…”

Section: Polyphasic Characterization Of Fungal Isolatesmentioning

confidence: 99%

Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria

et al. 2020

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Low moisture content ready-to-eat foods vended in Nigerian markets could be prepackaged or packaged at point of sale. These foods are widely and frequently consumed across Nigeria as quick foods. Despite their importance in the daily diets of Nigerians, a comprehensive study on the diversity of fungi, fungal metabolite production potential, and mycotoxin contamination in the foods has not yet been reported. Therefore, this study assessed the diversity of fungi in 70 samples of low moisture content ready-to-eat foods [cheese balls, garri (cassava-based), granola (a mix of cereals and nuts) and popcorn] in Nigeria by applying a polyphasic approach including morphological examination, genera/species-specific gene marker sequencing and secondary metabolite profiling of fungal cultures. Additionally, mycotoxin levels in the foods were determined by LC-MS/MS. Fungal strains (n = 148) were recovered only from garri. Molecular analysis of 107 representative isolates revealed 27 species belonging to 12 genera: Acremonium, Allophoma, Aspergillus, Cladosporium, Fusarium, Microdochium, Penicillium, Sarocladium, Talaromyces, and Tolypocladium in the Ascomycota, and Fomitopsis and Trametes in the Basidiomycota. To the best of our knowledge Allophoma, Fomitopsis, Microdochium, Tolypocladium, and Trametes are reported in African food for the first time. A total of 21 uncommon metabolites were found in cultures of the following species: andrastin A and sporogen AO1 in Aspergillus flavus; paspalin in A. brunneoviolaceus; lecanoic acid and rugulusovin in A. sydowii; sclerotin A in P. citrinum and Talaromyces siamensis; barceloneic acid, festuclavine, fumigaclavine, isochromophilons (IV, VI, and IX), ochrephilone, sclerotioramin, and sclerotiorin in P. sclerotium; epoxyagroclavine, infectopyron, methylorsellinic acid and trichodermamide C in P. steckii; moniliformin and sporogen AO1 in P. copticola; and aminodimethyloctadecanol in Tolypocladium. Twenty-four mycotoxins in addition to other 73 fungal and plant toxins were quantified in the foods. In garri, cheeseballs, popcorn and granola were 1, 6, 12, and 23 mycotoxins detected, respectively. Frontiers in Microbiology | www.frontiersin.org 1 April 2020 | Volume 11 | Article 615 Ezekiel et al. Fungi and Mycotoxins in Ready-to-Eat FoodsDeoxynivalenol, fumonisins, moniliformin, aflatoxins and citrinin contaminated 37, 31, 31, 20, and 14% of all food samples, respectively. Overall, citrinin had the highest mean concentration of 1481 µg/kg in the foods, suggesting high citrinin exposures in the Nigerian populace. Fungal and mycotoxin contamination of the foods depend on pre-food and post-food processing practices.

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Section: Polyphasic Characterization Of Fungal Isolatesmentioning

confidence: 99%

Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria

et al. 2020

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“…Phoma -like asexual morphs have been shown to be scattered within the Pleosporineae, Pleosporales (Chen et al 2017, Valenzuela-Lopez et al 2018). While trying to resolve the natural classification of Phoma -like species in Pleosporales, several new families have been introduced within the sub-order Pleosporineae by various authors (Zhang et al 2009, 2012, Hyde et al 2013, Ariyawansa et al 2015, Hernández-Restrepo et al 2017, Valenzuela-Lopez et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Ariyawansa¹,

Phillips²,

Chuang³

et al. 2018

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The order Pleosporales comprises a miscellaneous group of fungi and is considered to be the largest order of the class Dothideomycetes. The circumscription of Pleosporales has undergone numerous changes in recent years due to the addition of large numbers of families reported from various habitats and with a large amount of morphological variation. Many asexual genera have been reported in Pleosporales and can be either hyphomycetes or coelomycetes. Phoma-like taxa are common and have been shown to be polyphyletic within the order and allied with several sexual genera. During the exploration of biodiversity of pleosporalean fungi in Taiwan, a fungal strain was isolated from mycelium growing on the fruiting body of an Ophiocordyceps species. Fruiting structures that developed on PDA were morphologically similar to Phoma and its relatives in having pycnidial conidiomata with hyaline conidia. The fungus is characterised by holoblastic, cylindrical, aseptate conidiogenous cells and cylindrical, hyaline, aseptate, guttulated, thin-walled conidia. Phylogenetic analysis based on six genes, ITS, LSU, rpb2, SSU, tef1 and tub2, produced a phylogenetic tree with the newly generated sequences grouping in a distinct clade separate from all of the known families. Therefore, a new pleosporalean family Tzeananiaceae is established to accommodate the monotypic genus Tzeanania and the species T.taiwanensis in Pleosporales, Dothideomycetes. The Ophiocordyceps species was identified as O.macroacicularis and this is a new record in Taiwan.

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“…Note: The strain IRAN 3358C that was isolated from a diseased leaf of E. milii clustered with the ex‐isotype CBS 436.75 (=DSM 63365) strain of A. tropica that was previously recombined by Chen et al () based on a multigene phylogenetic analysis. This species was formerly reported from India, Italy, Western Europe, South America and the Netherlands (Boerema et al, ; Garibaldi et al, ; Nagarjun & Suryanarayana, ; Schneider & Boerema, ).…”

Section: Resultsmentioning

confidence: 99%

“…The fungal structures were photographed with an OLYMPUS BX51 microscope fitted with an OLYMPUS DP12 digital camera. The preliminary identification was carried out based on colony, pycnidial and conidial morphology (Boerema et al, ; Chen et al, , ; Valenzuela‐Lopez et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The LSU‐D1/D2, ITS and tub2 nucleotide sequences were compared to those of references in GenBank using a multi‐locus phylogenetic analysis. The combined data set constructed for LSU‐D1/D2, ITS and tub2 (three‐locus) contained all the sequences generated in this study and the known species of Allophoma and Ectophoma (Chen et al, , ; Valenzuela‐Lopez et al, ), as well as the outgroup taxon Paraboeremia selaginellae (Table ). To infer phylogenies, the LSU‐D1/D2, ITS and tub2 data sets were analysed separately, as well as combined (three‐locus data set).…”

Section: Methodsmentioning

confidence: 99%

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Ectophoma iranica sp. nov. and new hosts and records of Allophoma spp. in Iran

Larki

Mehrabi‐Koushki

Farokhinejad

2019

Journal of Phytopathology

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A survey was conducted to identify fungi associated with leaf diseases of ornamental herbs, including Christ plant, dracaena, rose geranium, rose periwinkle and treasure flower, in Ahvaz, southwestern Iran. Twenty‐five symptomatic herbs were collected and studied, which led to the isolation of seven Phoma‐like strains belonging to four taxa. These fungi were characterised based on DNA sequence data for the partial large subunit 28S nrDNA (LSU‐D1/D2), the internal transcribed spacer 1 and 2 and 5.8S nrDNA (ITS) and part of the β‐tubulin (tub2) gene regions. A multi‐locus‐based phylogeny, in combination with morphology, allowed for the identification of Allophoma hayatii, A. labilis, A. tropica and a novel species of the genus Ectophoma. Ectophoma iranica sp. nov. is morphologically and genetically distinctive from previously described species. In pathogenicity tests, the two strains of E. iranica (CBS 144681 and IRAN 3354C) caused leaf spot symptoms on leaves of Catharanthus roseus. New host plant species and records for A. hayatii, A. labilis and A. tropica are reported, plus further emended characterization of A. hayatii.

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Didymellaceae revisited

Cited by 200 publications

References 36 publications

Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria

Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Ectophoma iranica sp. nov. and new hosts and records of Allophoma spp. in Iran

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