Dicarbonyls Stimulate Cellular Protection Systems in Primary Rat Hepatocytes and Show Anti‐inflammatory Properties

Abstract

Advanced glycation endproducts (AGEs) and their precursor dicarbonyls are generally perceived as having adverse health effects. They are also considered to be initiators and promoters of disease and aging. However, proof for a causal relationship is lacking. On the other hand, it is known that AGEs and melanoidins possess beneficial properties, such as antioxidant and metal-chelating activities. Furthermore, some AGEs may stimulate the cellular detoxification system, generally known as the phase II drug metabolizing system. We show here that several reactive dicarbonyl intermediates have the capability to stimulate the cellular phase II detoxification systems in both a reporter cell line and primary rat hepatocytes. In addition, we demonstrate that dicarbonyls can attenuate the inflammatory signaling induced by tumor necrosis factor-alpha in a reporter cell system.

Produtos da reação de Maillard em alimentos: implicações para a saúde

“…Enquanto há evidên-cias de que altas concentrações de PRM no intestino aumentam a concentração local de nitrogênio, favorecendo o desenvolvimento de doenças intestinais como colite, alguns PRM, por outro lado, apresentam efeito prebiótico, indicando que o tema é controverso e pouco se conhece sobre a biotransformação desses compostos pela microbiota intestinal [22][23][24][25] . Nos últimos 50 anos o consumo desses produtos aumentou cerca de cinquenta vezes na dieta ocidental 24,25 , em especial na alimentação de adolescentes 26 , o que tem levado diversos autores a sugerir a necessidade de estabelecer limites de ingestão, ou, ainda, utilizar métodos culinários que reconhecidamente minimizem a formação desses compostos 14,20,27,28 .…”

“…O processamento térmico de cereais matinais leva à perda de 20% a 30% da lisina e à inibição da atividade de enzimas digestivas 28 . Como a lisina é um aminoácido limitante em produtos à base de cereais, as condições de manufatura devem ser escolhidas cuidadosamente, visando a garantir não apenas as características sensoriais, mas também o valor nutricional adequado 13,26,29 .…”

Chemical changes in aging Drosophila melanogaster

“…dicarbonyl compounds) and a wide variety of chemically stable adducts and crosslinks, known collectively as advanced glycation endproducts or AGEs (Gasser and Forbes 2008). Both dicarbonyl species and AGEs themselves are substrates for cellular Phase 2 detoxification systems (Buetler et al 2008;Hofmann et al 2001;Wenzel et al 2002). However, AGE-adducted proteins are highly degradation resistant under normal physiological conditions due to the thermodynamic stability of the crosslinks and adducted residues.…”

Multiple roles of glyoxalase 1-mediated suppression of methylglyoxal glycation in cancer biology—Involvement in tumour suppression, tumour growth, multidrug resistance and target for chemotherapy

“…Early evidence of the effect of AKR activity on potency of Glo1 inhibitors was that an aldose inhibitor Sorbinil potentiated the growth inhibitory response of BBGCp 2 in HL60 cells in vitro [7]. There is also the risk that increase of MG may induce Glo1 expression [22,76] but this would be circumvented with a high dose of Glo1 inhibitor that kills cells before significant induction of Glo1 expression can occur.…”