<i>DGAT1, DGAT2</i> and <i>PDAT</i> Expression in Seeds and Other Tissues of Epoxy and Hydroxy Fatty Acid Accumulating Plants

Li, Runzhi; Yu, Keshun; Hildebrand, David F.

doi:10.1007/s11745-010-3385-4

Cited by 142 publications

(121 citation statements)

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“…m e a n _ W T m e a n _ D (Li et al 2010b;Turchetto-Zolet et al 2011). Our results show that both JcDGAT1 and JcDGAT2 were able to restore TAG synthesis in the yeast cell and tobacco, reflecting the functional similarity of JcDGAT1 and JcDGAT2 in TAG biosynthesis.…”

Section: Discussionmentioning

confidence: 58%

“…To our knowledge, this is the first report on the substrate preference of DGAT2 to linoleic acid in plants. Previous studies also showed that RcDGAT2, VgDGAT2 and VfDGAT2 played a major role in TAG assembly of hydroxy or epoxy fatty acids in developing seeds (Shockey et al 2006;Burgal et al 2008;Li et al 2010b). It seems that DGAT2s have a greater contribution to accumulation of specific fatty acid compositions of TAG in planta than DGAT1s.…”

Section: Discussionmentioning

confidence: 91%

“…In Arabidopsis, AtDGAT1 controls TAG biosynthesis in developing seeds, whereas AtDGAT2 seems to have no obvious phenotype functionally (Shockey et al 2006;Zhang et al 2009). Similarly, the DGAT1s significantly regulate TAG accumulation in maize (Zheng et al 2008), Tropaeolum majus L. (Xu et al 2008) and soybean (Li et al 2010b). Further, studies have shown that the DGAT2s play a critical role in the accumulation of unusual FAs (such as epoxy and hydroxy fatty acids) in castor bean (Kroon et al 2006), tung tree (Shockey et al 2006) and Vernonia (Li et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

“…Further, studies have shown that the DGAT2s play a critical role in the accumulation of unusual FAs (such as epoxy and hydroxy fatty acids) in castor bean (Kroon et al 2006), tung tree (Shockey et al 2006) and Vernonia (Li et al 2010a). In addition, temporal and spatial expression patterns of DGAT1s and DGAT2s among organs or in different stages of seed development often display obvious differences (Li et al 2010b;Banilas et al 2011;Xu et al 2011). However, little is known about the functional similarity or divergence of DGAT1 and DGAT2 during the pathway of storage lipid biosynthesis in planta.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Yang

Wang

et al. 2014

Functional Plant Biol.

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Abstract. Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Yang

Wang

et al. 2014

Functional Plant Biol.

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“…Many efforts from both industrial and academic laboratories have been directed toward producing oxygenated fatty acids in oilseed crops. Current oilseed crops, such as soybean, rapeseed, and sunflower, do not produce hydroxy fatty acids or any other oxygenated fatty acid naturally (43). Yeast cells expressing At5g23940 were grown in the medium containing hydroxy fatty acid, which resulted in the incorporation of fatty acid into TAG.…”

Section: Gc-ms Analysis By Feeding the Cells With 16-hydroxyhexadecanoicmentioning

confidence: 99%

Defective in Cuticular Ridges (DCR) of Arabidopsis thaliana, a Gene Associated with Surface Cutin Formation, Encodes a Soluble Diacylglycerol Acyltransferase

Sapa

Krishna

Saha

et al. 2010

Journal of Biological Chemistry

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A key step in the triacylglycerol (TAG) biosynthetic pathway is the final acylation of diacylglycerol (DAG) by DAG acyltransferase. In silico analysis has revealed that the DCR (defective in cuticular ridges) (At5g23940) gene has a typical HX 4 D acyltransferase motif at the N-terminal end and a lipid binding motif VX 2 GF at the middle of the sequence. To understand the biochemical function, the gene was overexpressed in Escherichia coli, and the purified recombinant protein was found to acylate DAG specifically in an acyl-CoA-dependent manner. Overexpression of At5g23940 in a Saccharomyces cerevisiae quadruple mutant deficient in DAG acyltransferases resulted in TAG accumulation. At5g23940 rescued the growth of this quadruple mutant in the oleate-containing medium, whereas empty vector control did not. Lipid particles were localized in the cytosol of At5g23940-transformed quadruple mutant cells, as observed by oil red O staining. There was an incorporation of 16-hydroxyhexadecanoic acid into TAG in At5g23940-transformed cells of quadruple mutant. Here we report a soluble acyl-CoA-dependent DAG acyltransferase from Arabidopsis thaliana. Taken together, these data suggest that a broad specific DAG acyltransferase may be involved in the cutin as well as in the TAG biosynthesis by supplying hydroxy fatty acid. Triacylglycerol (TAG),3 an acyl ester glycerol, is the most efficient storage form of energy in eukaryotic cells (1, 2). In plants, TAG is the major component of the seed oils and is mainly deposited during seed and fruit development. Plant oils have a tremendous socioeconomic value in the food industry as well as in the production of lubricants and biofuels. They are also becoming increasingly important raw materials in the petrochemical industry as a substitute for non-renewable crude oil reserves (3). Thus, numerous conventional and molecular genetic strategies have been explored in attempts to increase the TAG content and modify the fatty acid composition of the plant seed oils. Isolation of the genes involved in oil biosynthesis offer exciting opportunities for overexpressing the desired gene of interest so as to redesign the plant oil metabolism for greater yields (3).TAG is usually synthesized by the sequential incorporation of acyl groups to glycerol 3-phosphate by substrate-specific acyltransferases to give lysophosphatidic acid and phosphatidic acid, respectively (4, 5). The synthesized phosphatidic acid is dephosphorylated to diacylglycerol (DAG). The last step in this pathway is catalyzed by acyl-CoA:DAG acyltransferase (DGAT; EC 2.3.1.20) that transfers an acyl group to DAG to form the TAG. DAG lies at the branch point between phospholipid and storage lipid biosynthesis. Therefore, much research has been focused on the acyl-CoA-dependent reaction catalyzed by the DAG acyltransferase, which is an integral endoplasmic reticulum protein (6) and has also been shown to be present in the oil bodies (7) and plastids (8). Several lines of evidence suggest that the TAG biosynthesis and DGAT activity are ...

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Lipid Biosynthesis

Hildebrand¹

2011

Plant Metabolism and Biotechnology

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DGAT1, DGAT2 and PDAT Expression in Seeds and Other Tissues of Epoxy and Hydroxy Fatty Acid Accumulating Plants

Cited by 142 publications

References 69 publications

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Defective in Cuticular Ridges (DCR) of Arabidopsis thaliana, a Gene Associated with Surface Cutin Formation, Encodes a Soluble Diacylglycerol Acyltransferase

Lipid Biosynthesis

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