<i>Desulfotomaculum</i>
            and
            <i>Methanobacterium</i>
            spp. Dominate a 4- to 5-Kilometer-Deep Fault

Moser, Duane P.; Gihring, Thomas M.; Brockman, Fred J.; Fredrickson, James K.; Balkwill, David L.; Dollhopf, Michael E.; Lollar, Barbara Sherwood; Pratt, Lisa M.; Boice, Erik; Southam, Gordon; Wanger, Greg; Baker, Brett J.; Pfiffner, Susan M.; Lin, Li; Onstott, Tullis C.

doi:10.1128/aem.71.12.8773-8783.2005

Cited by 163 publications

(170 citation statements)

References 70 publications

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“…The Eh of Ev818H6 was measured to be − 128 mV (Davidson et al, 2011). step in methanogenesis, was identified primarily within the classes Methanococci and Methanobacteria representing 71.5% and 16.7% of methylcoenzyme M reductase PEGs, respectively (Figure 1). Although the methanogens in TT107 are relatively more numerous than other sites within the Witwatersrand Basin, the FEF for biological CH 4 production (−9.8 × 10 − 15 kJ cell − 1 s − 1 ; Table 3) is less thermodynamically favorable than what has been reported for Dr938H3 (FEF as high as 1.0 × 10 − 13 kJ cell − 1 s − 1 ) (Moser et al, 2005) and Mponeng mine (FEF as high as 3.7 × 10 − 14 kJ cell − 1 s − 1 ) (Lin et al, 2006b). These differences reflect the higher H 2 concentrations found in Dr938H3 and MP104 relative to that of TT107.…”

Section: Deep Subsurface Metabolism C Magnabosco Et Almentioning

confidence: 49%

“…D. audaxviator', have been shown to exhibit both heterotrophic and autotrophic lifestyles, depending on the conditions (Londry et al, 2004;Rabus et al, 2006;Chivian et al, 2008). The flexibility of individual microorganisms to perform both heterotrophy and autotrophy has been suggested to be a critical survival adaptation for hydrogeologically isolated systems as the carbon substrate concentrations may change over time (Moser et al, 2005) and flexibility may also be an important feature of keystone species (for example, 'Ca. D. audaxviator') inhabiting the energy-limited TT107 fracture water.…”

Section: Geochemical Characteristicsmentioning

confidence: 99%

“…'Ca. D. audaxviator' also comprised 98-100% of the 96 bacterial clones library (90% of the total prokaryotic community) of the Dr938H3 borehole (Moser et al, 2005) located 1.7 km east of TT107. Although identified in the TT107 metagenome, 'Ca.…”

Section: Firmicutes-dominated Communitymentioning

confidence: 99%

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A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

et al. 2015

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Subsurface microbial communities comprise a significant fraction of the global prokaryotic biomass; however, the carbon metabolisms that support the deep biosphere have been relatively unexplored. In order to determine the predominant carbon metabolisms within a 3-km deep fracture fluid system accessed via the Tau Tona gold mine (Witwatersrand Basin, South Africa), metagenomic and thermodynamic analyses were combined. Within our system of study, the energy-conserving reductive acetyl-CoA (Wood-Ljungdahl) pathway was found to be the most abundant carbon fixation pathway identified in the metagenome. Carbon monoxide dehydrogenase genes that have the potential to participate in (1) both autotrophic and heterotrophic metabolisms through the reversible oxidization of CO and subsequent transfer of electrons for sulfate reduction, (2) direct utilization of H 2 and (3) methanogenesis were identified. The most abundant members of the metagenome belonged to Euryarchaeota (22%) and Firmicutes (57%)-by far, the highest relative abundance of Euryarchaeota yet reported from deep fracture fluids in South Africa and one of only five Firmicutes-dominated deep fracture fluids identified in the region. Importantly, by combining the metagenomics data and thermodynamic modeling of this study with previously published isotopic and community composition data from the South African subsurface, we are able to demonstrate that Firmicutes-dominated communities are associated with a particular hydrogeologic environment, specifically the older, more saline and more reducing waters.

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Section: Deep Subsurface Metabolism C Magnabosco Et Almentioning

confidence: 49%

Section: Geochemical Characteristicsmentioning

confidence: 99%

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A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

et al. 2015

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“…Considering only some of the recently published papers, SRB have been found in many deep geological formations including a 4-5 km deep fault (Moser et al, 2005), deep aquifers in Australia (Kimura et al, 2005) and France (Basso et al, 2005b(Basso et al, , 2009, deeply buried marine sediments (Schippers & Neretin, 2006) and oil reservoirs (Grabowski et al, 2005;Magot, 2005;Birkeland, 2005). From the numerous observations already published, it appears that SRB may constitute an important, or even major, component of the subterrestrial biosphere, which is considered to be possibly the largest prokaryotic habitat on our planet (Whitman et al, 1998;Parkes et al, 2005).…”

mentioning

confidence: 99%

Desulfocurvus vexinensis gen. nov., sp. nov., a sulfate-reducing bacterium isolated from a deep subsurface aquifer

Klouche

Basso

Lascourrèges

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…8, (9)). As D. audaxviator is reported to utilize radiochemically-produced substrates in the very deep continental subsurface (9,29,30,42), the appearance of a close relative at radioactive, subsurface habitats at the NTS was used in part to justify a full proposal led by the PI Additionally, results from this project and our relationship with Mavrik Zavarin and others from the the UGTA program supported our involvement at the co-I level in a full proposal to the ERSP National Laboratories Science Focus Areas call (DE-PS02-09ER09-07), Geochemical Processes at Femtomolar Concentrations and Nanometer Scales. This was ultimately approved for funding at near its $6,000,000 request and commenced in March of 2010.…”

Section: New Proposals Projects and Professional Relationshipsmentioning

confidence: 99%

Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site

Moser¹,

Russell²,

Marshall³

et al. 2008

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This US Department of Energy (DOE) Environmental Remediation Sciences Project (ERSP) was designed to test fundamental hypotheses concerning the existence and nature of indigenous microbial populations of Nevada Test Site subsurface nuclear test/detonation cavities. Now called Subsurface Biogeochemical Research (SBR), this program's Exploratory Research (ER) element, which funded this research, is designed to support high risk, high potential reward projects. Here, five cavities (GASCON, CHANCELLOR, NASH, ALEMAN, and ALMENDRO) and one tunnel (U12N) were sampled using bailers or pumps. Molecular and cultivation-based techniques revealed bacterial signatures at five sites (CHANCELLOR may be lifeless). SSU rRNA gene libraries contained diverse and divergent microbial sequences affiliated with known metal-and sulfur-cycling microorganisms, organic compound degraders, microorganisms from deep mines, and bacteria involved in selenate reduction and arsenite oxidation. Close relatives of Desulforudis audaxviator, a microorganism thought to subsist in the terrestrial deep subsurface on H 2 and SO 4 2-produced by radiochemical reactions, was detected in the tunnel waters. NTS-specific media formulations were used to culture and quantify nitrate-, sulfate-, iron-reducing, fermentative, and methanogenic microorganisms. Given that redox manipulations mediated by microorganisms can impact the mobility of DOE contaminants, our results should have implications for management strategies at this and other DOE sites. CONTENTS

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Desulfotomaculum and Methanobacterium spp. Dominate a 4- to 5-Kilometer-Deep Fault

Cited by 163 publications

References 70 publications

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

Desulfocurvus vexinensis gen. nov., sp. nov., a sulfate-reducing bacterium isolated from a deep subsurface aquifer

Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site

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