<i>Delftia tsuruhatensis,</i> an Emergent Opportunistic Healthcare-Associated Pathogen

Ranc, Alexandre; Dubourg, Grégory; Fournier, Pierre‐Edouard; Raoult, Didier; Fénollar, Florence

doi:10.3201/eid2403.160939

Cited by 33 publications

(34 citation statements)

References 7 publications

(8 reference statements)

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“…Another undesirable isolate was D. tsuruhatensis (NPL224), a plant‐growth‐promoting bacterium (Han et al, ) that has been implicated in some cases of human infection (Preiswerk, Ullrich, Speich, Bloemberg, & Hombach, ; Tabak et al, ). Resistance to β‐lactams and susceptibility to cefepime, as found in our strain, was reported earlier (Preiswerk et al, ), while susceptibility to ofloxacin was also reported by (Ranc, Dubourg, Fournier, Raoult, & Fenollar, ). A boron‐tolerant bacterium, L. boronitolerans (Ahmed, Yokota, Yamazoe, & Fujiwara, ), was found in nondairy fermented products (Nam, Seo, Lim, & Lee, ) and was susceptible to most antibiotics.…”

Section: Discussionsupporting

confidence: 90%

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Aziz

Zaidi

Bakht

et al. 2019

Journal of Food Safety

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Yogurt is a health food with notable market production and demand. Because of this, we conducted a study on prominent commercial brands of yogurts in Pakistan for microbial content and the probiotic potential of the contained lactic acid bacteria (LAB), in the context of their label claims. All contained viable LAB, but the numbers (cfu g −1 ) varied considerably. Three of the products made explicit probiotic claims, but LAB from these displayed no probiotic attributes per WHO-FAO guidelines. The yogurt starter and nonstarter Lactobacillus strains had no gelatinase or hemolytic activity and exhibited significant antibacterial activity against some human pathogens. One brand with a probiotic claim contained an L. acidophilus strain that showed cholesterol assimilation activity in vitro. Some potential human pathogens that were hemolytic and resistance to β-lactam antibiotics were also detected in the products. The findings demonstrate a need for better quality control and regulation to ensure safety and efficacy of yogurt products.Values are mean of three replicates. ‡ LAB isolated on MRSc and M17 media. | Non-LAB isolated on nutrient and MacConkey media. § Genus and species-specific primers for Lactobacillus, Bifidobacterium, Lactobacillus delbrueckii, Lactococcus lactis, and Streptococcus thermophilu. ¶ Expiry and manufacturing date and contact information provided except lot/batch number. **a: de Man, Rogosa and Sharpe (MRS) medium supplemented with L-cysteine in anaerobic condition; b: M17 in aerobic conditions. † † Neg for the primers used.

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Section: Discussionsupporting

confidence: 90%

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Aziz

Zaidi

Bakht

et al. 2019

Journal of Food Safety

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“…Our understanding of Delftia tsuruhatensis has experienced a complete turnaround from the promising plant growth-promoting bacteria to emerging human pathogen predominantly isolated from respiratory specimens, blood and urine 27,30 . There is a disparity between the growing importance of Delftia spp.…”

Section: Discussionmentioning

confidence: 99%

“…Delftia tsuruhatensis strains have previously been investigated as plant growth-promoting rhizobacteria (PGPR) due to their production of siderophores which can mitigate iron limitation in soil 27 . However, recent findings pinpoint D. tsuruhtensis as an emerging pathogen associated with an increasing number of human infections 28–30 . It was previously published that D. tsuruhatensis could exhibit an anti-quorum sensing activity to P. aeruginosa quorum sensing systems, and authors identified a diisooctyl ester of 1,2 benzenedicarboxylic acid as an active compound 31 .…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Malešević

Lorenzo

Filipić

et al. 2019

Sci Rep

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Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.

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“…In bald eagles, the most common species belonging to this phylum were tentatively classified as Burkholderia ubonensis and Delftia tsuruhatensis , comprising 18% and 10% of all fecal bacteria detected respectively. Both are common environmental bacteria that have been occasionally linked to infections in humans [38, 39]. B. ubonensis was also detected in the two common ravens sampled, ranking seventh most common and averaging 5.6%.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Diversity in Feces of Wild Bald Eagles, Turkey Vultures and Common Ravens from the Pacific Northwest Coast, U.S.A

Crespo

Dowd²,

Varland³

et al. 2019

Preprint

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RC) 28 29 30 ¶ These authors contributed equally to this work. 2 31 Abstract 32Birds harbor diverse microorganisms in their guts, which collectively fulfill important 33 roles in providing their hosts with nutrition and protection from pathogens. Although numerous 34 studies have investigated the presence of certain pathogenic bacteria in the feces of wild birds, 35 only a few have attempted to investigate the microbiota of the gut. This study analyzed the avian 36 bacteria present in the cloaca of avian scavengers captured on coastal beaches of Washington and 37 Oregon between 2013 and 2015: 10 turkey vultures (Cathartes aura), 9 bald eagles (Haliaeetus 38 leucocephalus), and 2 common ravens (Corvus corax). We used illumina sequencing based on 39 the V4 region of the 16s gene was to characterize the bacterial diversity. Our investigation 40 revealed phylum-level differences in the microbiome of turkey vultures, compared with bald 41 eagles and common ravens. Substantial microbiome differences were found between bald eagles 42 and ravens below the phylum level. Although little is known about the possible relations among 43 these microorganisms, our analyses provides the first integrated look at the composition of the 44 avian microbiota and serves as a foundation for future studies in this area. 45 46 Keywords: avian scavenger, turkey vulture, bald eagle, common raven, bacteria, coast, 47 microbiota, microbiome, raptor. 48 Abbreviations: OTU = operational taxonomic unit 49 50 3 51 Introduction 52 Birds harbor diverse microorganisms in their gut, which collectively fulfill important 53 roles in providing their hosts with nutrition and protection from pathogens [1]. Gut 54 microorganisms have been isolated and characterized by a variety of methods, such as culture-55 based assays, culture-independent DNA sequencing, and fluorescence in situ hybridization 56 targeting the 16S rRNA gene [2]. Most of these studies are limited in scope and are focused on 57 pathogenic bacteria such as Salmonella, Escherichia coli, Campylobacter, and Clostridium 58 perfringens [3-5]. 59 In recent years, metagenomics using next-generation sequencing has emerged as a way to 60 analyze complex microbial communities and their functions [6]. This approach is capable of 61 sequencing thousands or millions of amplified DNA molecules in a single run and, for bacteria, 62 does not require conventional cloning and amplification. Additionally, metagenomic studies 63 offer a powerful tool for the comprehensive and unbiased assessment of microbial diversity 64 within the complex gut ecosystem by allowing examination of organisms not easily cultured in 65 the laboratory [6, 7]. 66 Few studies have used genetic sequencing to explore the intestinal microbiota of wild 67 birds [8]. Furthermore, these studies reported gene sequences to phylum or class levels only, 68 with no attempt to understand extant microorganism populations at the species level. In this 69 study, we analyze the avian microbiome present in the cloaca of bald eagles, turkey vultures, ...

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Delftia tsuruhatensis, an Emergent Opportunistic Healthcare-Associated Pathogen

Cited by 33 publications

References 7 publications

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Bacterial Diversity in Feces of Wild Bald Eagles, Turkey Vultures and Common Ravens from the Pacific Northwest Coast, U.S.A

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