<i>Data Analysis WorkbeNch</i>(<i>DAWN</i>)

Basham, Mark; Filik, Jacob; Wharmby, Michael T.; Chang, Peter; Kassaby, Baha El; Gerring, Matthew; Aishima, Jun; Levik, Karl; Pulford, Bill; Sikharulidze, Irakli; Sneddon, Duncan; Webber, Matthew J.; Dhesi, S. S.; Maccherozzi, Francesco; Svensson, Olof; Brockhauser, Sándor; Náray, Gabor; Ashton, Alun

doi:10.1107/s1600577515002283

Cited by 391 publications

(307 citation statements)

References 14 publications

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“…The data was then corrected to allow for sample transmission and background scattering (using 20 mM Tris, 150 mM NaCl at pH 7.5 in ddH 2 O as a reference). The data were processed using the DAWN software package (Diamond Light Source Ltd., UK) [28].…”

Section: Saxs Data Collectionmentioning

confidence: 99%

Monomeric green fluorescent protein as a protein standard for small angle scattering

Myatt

Hatter

Rogers

et al. 2017

BSI

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Abstract. Protein small angle scattering (SAS) has become increasing important in structural biochemistry, due to the increased performance and specification of new instruments and advances in the software and hardware used to analyse the data. Whilst all of this is encouraging, there is a lack of standardised experimental methodology within the community. Although a number of protein standards are currently used in SAS experiments to allow accurate molecular weight determination, each has specific advantages and disadvantages. We therefore propose the use of a mutated monomeric enhanced green fluorescent protein, as a protein standard, abbreviated to m-eGFP. It has a number of advantages over the currently used protein standards, for example it is cheap and easy to produce. It can be expressed in large amounts (>40 mg/L) in both hydrogenated and deuterated form. The mutation means it is highly monodisperse and GFP being a beta-barrel structure is thermodynamically stable over a number of days, giving highly reproducible results. We therefore believe m-eGFP is a good protein standard for small angle scattering (SAS).

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Section: Saxs Data Collectionmentioning

confidence: 99%

Monomeric green fluorescent protein as a protein standard for small angle scattering

Myatt

Hatter

Rogers

et al. 2017

BSI

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“…The 2-D diffraction patterns obtained were converted into 1-D intensity vs 2θ plots via azimuthal integration using DAWN [19]. Volume fractions and lattice parameters of ferrite and cementite for each pattern were obtained using manually guided fitting of the Rietveld refinement [20] in HighScore Plus [21].…”

Section: Synchrotron Measurementsmentioning

confidence: 99%

Synchrotron and neural network analysis of the influence of composition and heat treatment on the rolling contact fatigue of hypereutectoid pearlitic steels

Solano-Alvarez

Peet

Pickering

et al. 2017

Materials Science and Engineering: A

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A series of experimental hypereutectoid pearlitic steels were tested under rolling contact sliding conditions using a lubricated twin-disc setup to study the influence of different chemical compositions and heat treatments on rolling contact fatigue life. Tested samples were then characterised using microscopy and synchrotron measurements as a function of depth from the contact surface. Results, analysed through neural networks, indicate that the most influential factor in lengthening the number of cycles to crack initiation of hypereutectoid steels is hardness, attained by increasing the cooling rate from the hot rolling temperature, but adequate alloying additions can enhance it further. The harder, fast-cooled samples displayed less plastic flow at the surface than the softer slow-cooled ones. With regard to chemical composition, silicon was found to strengthen the ferrite thus reducing strain incompatibilities with the cementite, preventing in this way the fragmentation and eventual dissolution of the lamellae. This is beneficial since larger depths of cementite dissolution were found in samples with lower cycles to crack initiation for a given cooling rate (hardness). Samples containing vanadium lasted longer and displayed less plastic deformation at the surface than those without, at a similar hardness.

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“…The position of each diffraction pattern was correlated with a 'live' optical image of the corroding artificial pit to allow exact correlation of the diffraction pattern relative to the moving metal-electrolyte interface. X-ray diffraction patterns were integrated and analyzed in DAWN (Version 2.0.0, 2010-2016) 35 and FullProf software (Version 3.00, 2015).…”

Section: Methodsmentioning

confidence: 99%

In-Situ Synchrotron X-ray Characterization of Corrosion Products in Zr Artificial Pits in Simulated Physiological Solutions

Zhang

Addison

Gostin

et al. 2017

J. Electrochem. Soc.

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Corrosion products generated in artificial pits of zirconium were characterized in-situ by synchrotron X-ray diffraction and X-ray absorption near edge structure (XANES) in physiological saline, with and without addition of 4% albumin and/or 0.1% H 2 O 2 . Zr metal fragments and tetragonal ZrO 2 particles were detected in aggregated black corrosion products away from the corrosion front. At the corrosion front, a ZrOCl 2 ·8H 2 O salt layer of a few hundreds of microns thickness was formed. Coarsened ZrOCl 2 ·8H 2 O crystallites were found farther out into the solution. The Zr solution species were confirmed to be in a tetravalent state by XANES. TEM imaging of the corrosion products revealed heterogeneity of the morphology of the Zr metal fragments and confirmed their size to be less than a few microns. The formation and speciation of Zr corrosion products were found not affected by the presence of H 2 O 2 and/or albumin in physiological saline. Furthermore, bulk Zr electrochemistry identified that the presence of H 2 O 2 and/or albumin did not affect passive current densities and pitting potentials of the bulk Zr surface. Therefore, it is concluded that the pitting susceptibility and pit chemistry of Zr in physiological saline were unaffected by the presence of H 2 O 2 , albumin or their combinations.

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Data Analysis WorkbeNch(DAWN)

Abstract: DAWN is a generic data analysis software platform that has been developed for use at synchrotron beamlines for data visualization and analysis. Its generic design makes it suitable for use in a range of scientific and engineering applications.

Cited by 391 publications

References 14 publications

Monomeric green fluorescent protein as a protein standard for small angle scattering

Monomeric green fluorescent protein as a protein standard for small angle scattering

Synchrotron and neural network analysis of the influence of composition and heat treatment on the rolling contact fatigue of hypereutectoid pearlitic steels

In-Situ Synchrotron X-ray Characterization of Corrosion Products in Zr Artificial Pits in Simulated Physiological Solutions

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