CYP9A12andCYP9A17in the cotton bollworm,Helicoverpa armigera: sequence similarity, expression profile and xenobiotic response

Zhou, Xiaohe; Ma, Cun‐Gen; Mei, Li; Sheng, Cheng; Liu, Huixia; Qiu, Xinghui

doi:10.1002/ps.1832

Cited by 77 publications

(64 citation statements)

References 55 publications

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“…Instead, the most likely underlying mechanism for the lack of SCPI's biological effects in late larval stages (3rd and 4th) may be the high levels of detoxification enzymes such as P450s and GSTs expressed in the late larval stages. Elevated levels of detoxification enzymes in larvae may occur naturally due to increased expression levels of detoxification enzymes in late larval stages (Grubor and Heckel, 2007), and/or induced expression of detoxification enzymes by xenobiotic compounds (Zhou et al, 2010), such as SCPIs. Mangostin-induced P450 and GST activities in mosquito larvae have been reported (Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the sterol carrier protein-x/sterol carrier protein-2 gene in the cotton bollworm, Helicoverpa armigera

Zhang

et al. 2012

Journal of Insect Physiology

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Section: Discussionmentioning

confidence: 99%

Characterization of the sterol carrier protein-x/sterol carrier protein-2 gene in the cotton bollworm, Helicoverpa armigera

Zhang

et al. 2012

Journal of Insect Physiology

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“…In Helicoverpa armigera, deltamethrin induces transcription of two CYP9A subfamily members, although the functions of these have not yet been defined (45). One of these and another CYP9A subfamily member are constitutively overexpressed in laboratory strains selected for resistance to the pyrethroid fenvalerate (46).…”

Section: Discussionmentioning

confidence: 99%

CYP9Q-mediated detoxification of acaricides in the honey bee (Apis mellifera)

Mao¹,

Schuler

Berenbaum³

2011

Proc. Natl. Acad. Sci. U.S.A.

239

196

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Although Apis mellifera, the western honey bee, has long encountered pesticides when foraging in agricultural fields, for two decades it has encountered pesticides in-hive in the form of acaricides to control Varroa destructor, a devastating parasitic mite. The pyrethroid tau-fluvalinate and the organophosphate coumaphos have been used for Varroa control, with little knowledge of honey bee detoxification mechanisms. Cytochrome P450-mediated detoxification contributes to pyrethroid tolerance in many insects, but specific P450s responsible for pesticide detoxification in honey bees (indeed, in any hymenopteran pollinator) have not been defined. We expressed and assayed CYP3 clan midgut P450s and demonstrated that CYP9Q1, CYP9Q2, and CYP9Q3 metabolize tau-fluvalinate to a form suitable for further cleavage by the carboxylesterases that also contribute to tau-fluvalinate tolerance. These in vitro assays indicated that all of the three CYP9Q enzymes also detoxify coumaphos. Molecular models demonstrate that coumaphos and tau-fluvalinate fit into the same catalytic pocket, providing a possible explanation for the synergism observed between these two compounds. Induction of CYP9Q2 and CYP9Q3 transcripts by honey extracts suggested that diet-derived phytochemicals may be natural substrates and heterologous expression of CYP9Q3 confirmed activity against quercetin, a flavonoid ubiquitous in honey. Up-regulation by honey constituents suggests that diet may influence the ability of honey bees to detoxify pesticides. Quantitative RT-PCR assays demonstrated that tau-fluvalinate enhances CYP9Q3 transcripts, whereas the pyrethroid bifenthrin enhances CYP9Q1 and CYP9Q2 transcripts and represses CYP9Q3 transcripts. The independent regulation of these P450s can be useful for monitoring and differentiating between pesticide exposures in-hive and in agricultural fields.Apidae | miticide | transcriptional regulation

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“…Moreover, 13 additional P450 s are upregulated and two downregulated in the gut at the highest gossypol concentration. When CYP6AE14 was silenced in H. armigera by feeding dsRNA or transgenic cotton transformed with a construct expressing dsRNA, larval growth was greatly reduced in the presence of gossypol [21,22], however there are no heterologous expression studies providing information about CYP6AE14 substrate specificity and its direct role in gossypol metabolism. Racemic gossypol promotes the formation of superoxide free-radicals when incubated with rat liver microsomes [49].…”

Section: Discussionmentioning

confidence: 99%

“…Recent analyses for two animal systems (mammal and annelid) inquired whether gene expression in a global scale differs under the "low-dose effect" of toxic chemicals [18,19]. Dose-dependent transcriptional responses to gossypol have been observed for a handful of genes including the expression of mitochondrial-related genes in rat liver cells [20] and P450 monooxygenases in insects [21,22]. However, there is no unbiased transcriptional profiling approach documented investigating the effects of different doses of gossypol.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses underlying the hormetic and detrimental effects of the plant secondary metabolite gossypol on the generalist herbivore Helicoverpa armigera

et al. 2011

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BackgroundHormesis is a biphasic biological response characterized by the stimulatory effect at relatively low amounts of chemical compounds which are otherwise detrimental at higher concentrations. A hormetic response in larval growth rates has been observed in cotton-feeding insects in response to increasing concentrations of gossypol, a toxic metabolite found in the pigment glands of some plants in the family Malvaceae. We investigated the developmental effect of gossypol in the cotton bollworm, Helicoverpa armigera, an important heliothine pest species, by exposing larvae to different doses of this metabolite in their diet. In addition, we sought to determine the underlying transcriptional responses to different gossypol doses.ResultsLarval weight gain, pupal weight and larval development time were measured in feeding experiments and a hormetic response was seen for the first two characters. On the basis of net larval weight gain responses to gossypol, three concentrations (0%, 0.016% and 0.16%) were selected for transcript profiling in the gut and the rest of the body in a two-color double reference design microarray experiment. Hormesis could be observed at the transcript level, since at the low gossypol dose, genes involved in energy acquisition such as β-fructofuranosidases were up-regulated in the gut, and genes involved in cell adhesion were down-regulated in the body. Genes with products predicted to be integral to the membrane or associated with the proteasome core complex were significantly affected by the detrimental dose treatment in the body. Oxidoreductase activity-related genes were observed to be significantly altered in both tissues at the highest gossypol dose.ConclusionsThis study represents the first transcriptional profiling approach investigating the effects of different concentrations of gossypol in a lepidopteran species. H. armigera's transcriptional response to gossypol feeding is tissue- and dose-dependent and involves diverse detoxifying mechanisms not only to alleviate direct effects of gossypol but also indirect damage such as pH disturbance and oxygen radical formation. Genes discovered through this transcriptional approach may be additional candidates for understanding gossypol detoxification and coping with gossypol-induced stress. In a generalist herbivore that has evolved transcriptionally-regulated responses to a variety of different plant compounds, hormesis may be due to a lower induction threshold of growth-promoting, stress-coping responses and a higher induction threshold of detoxification pathways that are costly and cause collateral damage to the cell.

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CYP9A12andCYP9A17in the cotton bollworm,Helicoverpa armigera: sequence similarity, expression profile and xenobiotic response

Abstract: CYP9A12 and CYP9A17 showed high sequence identity, but with differential expression patterns, suggesting that CYP9A12 and CYP9A17 genes in H. armigera might diverge via subfunctionalisation after the gene duplication event.

Cited by 77 publications

References 55 publications

Characterization of the sterol carrier protein-x/sterol carrier protein-2 gene in the cotton bollworm, Helicoverpa armigera

Characterization of the sterol carrier protein-x/sterol carrier protein-2 gene in the cotton bollworm, Helicoverpa armigera

CYP9Q-mediated detoxification of acaricides in the honey bee (Apis mellifera)

Transcriptional responses underlying the hormetic and detrimental effects of the plant secondary metabolite gossypol on the generalist herbivore Helicoverpa armigera

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