<i>Curcuma longa</i> ethanol extract and Curcumin inhibit the growth of <i>Acanthamoeba triangularis</i> trophozoites and cysts isolated from water reservoirs at Walailak University, Thailand

Mitsuwan, Watcharapong; Bunsuwansakul, Chooseel; Leonard, Theodore Ebenezer; Laohaprapanon, Sawanya; Hounkong, Kruawan; Bunluepuech, Kingkan; Kaewjai, Chalermpon; Mahboob, Tabassum; Raju, Chandramathi Sumudi; Dhobi, Mahaveer; Pereira, Maria de Lourdes; Nawaz, Muhammad; Wiart, Christophe; Siyadatpanah, Abolghasem; Norouzi, Roghayeh; Nissapatorn, Veeranoot

doi:10.1080/20477724.2020.1755551

Cited by 18 publications

(32 citation statements)

References 25 publications

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“…Furthermore, dimethoxy curcumin, curcuminoids isolated from C. longa rhizomes, showed significant amoebicidal effects against A. castellanii ( Aqeel et al, 2012 ). Recently, both C. longa extract and Curcumin exhibited anti- Acanthamoeba activity against A. triangularis trophozoites and cysts as published by our research group ( Mitsuwan et al, 2020 ). Having considered the dearth of information on the relevance of natural products as an anti-adhesive agent against the adhesion of parasites, this study is therefore focused on the investigation of the anti-adhesion activity of C. longa extract and Curcumin against A. triangularis trophozoites and cysts in polystyrene plastic and contact lenses.…”

Section: Introductionmentioning

confidence: 72%

“…Both trophozoites and cysts cells harvested from PYG were cultured in PYG medium containing sub-minimal inhibitory concentrations (sub-MICs) of the extract and/or Curcumin. The concentrations including sub-MICs of the extract and Curcumin used in this study have been reported by our research team ( Mitsuwan et al, 2020 ). Chlorhexidine and 1% DMSO were included as positive and negative controls, respectively.…”

Section: Methodsmentioning

confidence: 97%

“…Acanthamoeba triangularis WU19001 was cultured in Non-nutrient agar plates (NNA plates) seeded with the suspension of Escherichia coli ( E. coli ) cells ( Mitsuwan et al, 2020 ). Trophozoite cells were harvested after 48–72 h of incubation at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens

Mitsuwan

Sangkanu

Romyasamit

et al. 2020

International Journal for Parasitology: Drugs and Drug Resistan

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Curcuma longa and Curcumin have been documented to have a wide spectrum of pharmacological effects, including anti- Acanthamoeba activity. Hence, this study sought to explore the anti-adhesion activity of C. longa extract and Curcumin against Acanthamoeba triangularis trophozoites and cysts in plastic and contact lenses. Our results showed that C. longa extract and Curcumin significantly inhibited the adhesion of A. triangularis trophozoites and cysts to the plastic surface, as investigated by the crystal violet assay (P < 0.05). Also, an 80–90% decrease in adhesion of trophozoites and cysts to the plastic surface was detected following the treatment with C. longa extract and Curcumin at 1/2 × MIC, compared to the control. In the contact lens model, approximately 1 log cells/mL of the trophozoites and cysts was reduced when the cells were treated with Curcumin, when compared to the control. Pre-treatment of the plastic surface with Curcumin at 1/2-MIC reduced 60% and 90% of the adhesion of trophozoites and cysts, respectively. The reduction in 1 Log cells/mL of the adhesion of A. triangularis trophozoites was observed when lenses were pre-treated with both the extract and Curcumin. Base on the results obtained from this study, A. triangularis trophozoites treated with C. longa extract and Curcumin have lost strong acanthopodia, thorn-like projection pseudopodia observed by scanning electron microscope. This study also revealed the therapeutic potentials of C. longa extract and Curcumin, as such, have promising anti-adhesive potential that can be used in the management/prevention of A. triangularis adhesion to contact lenses.

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Section: Introductionmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 97%

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Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens

Mitsuwan

Sangkanu

Romyasamit

et al. 2020

International Journal for Parasitology: Drugs and Drug Resistan

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“…A. triangularis WU19001, a strain from the recreational reservoir at Walailak University, Nakhon Si Thammarat Thailand was used in this study [11]. The parasite was grown in PYG medium (0.75% (w/v) proteose peptone, 0.75% (w/v) yeast extract and 1.5% (w/v) glucose).…”

Section: Cultivation Of a Triangularismentioning

confidence: 99%

“…The minimum inhibitory concentration (MIC) for plant extract was determined using microtiter broth dilution method [11]. Plant extract was diluted to give a nal concentration of 4000, 2000, 1000, 500, 250, 125, 62.5 µg/mL into 96-well microplate.…”

Section: Determination Of Minimal Inhibitory Concentration (Mic) Of Gmentioning

confidence: 99%

Synergistic Effect of Garcinia Mangostana Combined With Chlorhexidine on Acanthamoeba Triangularis Trophozoites and Cysts

Sangkanu

Mitsuwan

Mahabusarakam

et al. 2020

Preprint

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Background: Various parts of Garcinia mangostana Linn, including its pericarp have been traditionally used to treat different types of diseases. This study was carried out to determine the anti-Acanthamoeba activity of G. mangostana pericarp extract against Acanthamoeba triangularis. Methods: The G. mangostana ethanolic pericarp extract was screened for the anti-Acanthamoeba activity and determined its minimal inhibition concentrations (MICs) by the microdilution method. Then, the time-kill kinetic assay of the extract was determined. The synergistic effect of G. mangostana extract and chlorhexidine was performed using the checkerboard method. Parasite morphology was detected by scanning electron microscopy (SEM).Results: The MICs of extract were assessed on trophozoites and cysts with 250 and 4000 µg/mL, respectively. More so, at 2×MIC of extract exhibited inhibitory activity against trophozoites and cyst of A. triangularis for up to 7 days. Checkerboard assays showed synergistic activity of extract (500-1000 μg/mL) plus chlorhexidine (3.90-15.62 μg/mL) at a fractional inhibitory concentration index (FICI) of 0.18-0.37. The lowest FICI (0.18) displayed good synergism resulting in up to 16-fold reduction of drug MIC and reducing to 8-fold of extract MIC. The viability of cysts decreased to 12.28±3.03 cells/mL. FICI interpretation equal to 1 is considered an additive effect on Acanthamoeba trophpzoites. The SEM results clearly showed that Acanthamoeba cells treated with a single drug of chlorhexidine and its combination with G. mangostana extract caused cell membrane damage and irregular cell shapes comparing with the control. Conclusion: A good combinatorial relationship displayed by G. mangostana extract and chlorhexidine suggest a more reliable evidenced based therapeutic strategy. Therefore, this approach is promising and could be employed as an alternative treatment method for the management of Acanthamoeba infection.

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DNA Extraction from Trophozoites and Cysts Using Manual Protocol

Gohil,

Chandarana,

Jawahar

et al. 2024

Springer Protocols Handbooks

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Curcuma longa ethanol extract and Curcumin inhibit the growth of Acanthamoeba triangularis trophozoites and cysts isolated from water reservoirs at Walailak University, Thailand

Cited by 18 publications

References 25 publications

Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens

Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens

Synergistic Effect of Garcinia Mangostana Combined With Chlorhexidine on Acanthamoeba Triangularis Trophozoites and Cysts

DNA Extraction from Trophozoites and Cysts Using Manual Protocol

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