<i>Cstf2t</i> Regulates expression of histones and histone‐like proteins in male germ cells

Grozdanov, Petar; Li, J.; Yu, Peng; Yan, Wei; MacDonald, Clinton C.

doi:10.1111/andr.12488

Cited by 17 publications

(14 citation statements)

References 81 publications

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“…CstF64/tau is encoded by Cstf2t, a paralog of the Cstf2 gene that encodes CstF64. Cstf2t is expressed in germ cells, also has a role on polyadenylation, being indispensable for spermatogenesis in rats (Harris et al, 2016;Grozdanov et al, 2018a). CstF64/tau binds to sequences rich in U>G also downstream of the PAS (Mandel et al, 2008).…”

Section: Cleavage and Polyadenylation Molecular Complexmentioning

confidence: 99%

Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

Bernardes

Menossi

2020

Front. Plant Sci.

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Molecular biotechnology has made it possible to explore the potential of plants for different purposes. The 3' regulatory regions have a great diversity of cis-regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. A complex interaction between the cleavage and polyadenylation molecular complex and cis-elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. In addition, after transcription, a wide array of RNA-binding proteins interacts with cis-acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. In this review, we discuss the role of 3' regulatory regions in gene expression, and the superior potential that plant 3' regulatory regions have compared to NOS, OCS and 35S 3' regulatory regions.

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Section: Cleavage and Polyadenylation Molecular Complexmentioning

confidence: 99%

Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

Bernardes

Menossi

2020

Front. Plant Sci.

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“…Our laboratory has examined further roles for τCstF‐64 in global mRNA expression (Li et al, ), APA and splicing of the Crem mRNA (Grozdanov, Amatullah, Graber, & MacDonald, ), and expression of histone mRNAs (Grozdanov, Li, Yu, Yan, & MacDonald, ) in testis. We also found important roles for τCstF‐64 in embryonic stem cells (Youngblood et al, ) and during endoderm differentiation (Youngblood & MacDonald, ).…”

Section: Multiple Testis‐specific Polyadenylation Proteins Are Expresmentioning

confidence: 99%

Tissue‐specific mechanisms of alternative polyadenylation: Testis, brain, and beyond (2018 update)

MacDonald

2019

WIREs RNA

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Alternative polyadenylation (APA) is how genes choose different sites for 3′ end formation for mRNAs during transcription. APA often occurs in a tissue‐ or developmental stage‐specific manner that can significantly affect gene activity by changing the protein product generated, the stability of the transcript, its localization within the cell, or its translatability. Despite the important regulatory effects that APA has on tissue‐specific gene expression, only a few examples have been characterized mechanistically. In this 2018 update to our 2010 review, we examine mechanisms for the control of APA and update our understanding of the older mechanisms since 2010. We once postulated the existence of tissue‐specific factors in APA. However, while a few tissue‐specific polyadenylation factors are known, the emerging conclusion is that the majority of APA is accomplished by altering levels of core polyadenylation proteins. Examples of those core proteins include CSTF2, CPSF1, and subunits of mammalian cleavage factor I. But despite support for these mechanisms, no one has yet documented any of these proteins changing in either a tissue‐specific or developmental manner. Given the profound effect that APA can have on gene expression and human health, improved understanding of tissue‐specific APA could lead to numerous advances in gene activity control. This article is categorized under: RNA Processing > 3′ End Processing RNA in Disease and Development > RNA in Development

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“…For example, the gene Cleavage Stimulation Factor Subunit 2 Tau Variant ( Cstf2t ) has been demonstrated to be an important stage-specific regulator of Crem mRNA processing that controls Crem polyadenylation in mouse testis. Cstf2t can lead to an overall decrease of the Crem mRNAs generated from internal promoters in Cstf2t −/− mice ( 30 , 31 ). Therefore, these 140 RBPs can be promising candidates for RNA-Seq studies in the future.…”

Section: Resultsmentioning

confidence: 99%

RBPMetaDB: a comprehensive annotation of mouse RNA-Seq datasets with perturbations of RNA-binding proteins

Deng

Vieira

et al. 2018

Database

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RNA-binding proteins (RBPs) may play a critical role in gene regulation in various diseases or biological processes by controlling post-transcriptional events such as polyadenylation, splicing and mRNA stabilization via binding activities to RNA molecules. Owing to the importance of RBPs in gene regulation, a great number of studies have been conducted, resulting in a large amount of RNA-Seq datasets. However, these datasets usually do not have structured organization of metadata, which limits their potentially wide use. To bridge this gap, the metadata of a comprehensive set of publicly available mouse RNA-Seq datasets with perturbed RBPs were collected and integrated into a database called RBPMetaDB. This database contains 292 mouse RNA-Seq datasets for a comprehensive list of 187 RBPs. These RBPs account for only ∼10% of all known RBPs annotated in Gene Ontology, indicating that most are still unexplored using high-throughput sequencing. This negative information provides a great pool of candidate RBPs for biologists to conduct future experimental studies. In addition, we found that DNA-binding activities are significantly enriched among RBPs in RBPMetaDB, suggesting that prior studies of these DNA- and RNA-binding factors focus more on DNA-binding activities instead of RNA-binding activities. This result reveals the opportunity to efficiently reuse these data for investigation of the roles of their RNA-binding activities. A web application has also been implemented to enable easy access and wide use of RBPMetaDB. It is expected that RBPMetaDB will be a great resource for improving understanding of the biological roles of RBPs.Database URL: http://rbpmetadb.yubiolab.org

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Cstf2t Regulates expression of histones and histone‐like proteins in male germ cells

Cited by 17 publications

References 81 publications

Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

Tissue‐specific mechanisms of alternative polyadenylation: Testis, brain, and beyond (2018 update)

RBPMetaDB: a comprehensive annotation of mouse RNA-Seq datasets with perturbations of RNA-binding proteins

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