<i>Cryptosporidium parvum</i> antigens induce mouse and human dendritic cells to generate Th1‐enhancing cytokines

Bedi, Brahmchetna; Mead, Jan R.

doi:10.1111/j.1365-3024.2012.01382.x

Cited by 27 publications

(31 citation statements)

References 51 publications

(74 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies demonstrated the importance of TNF-α in C. parvum infection by showing that the cytokine significantly inhibited C. parvum development in enterocyte cell lines (Lacroix et al, 2001; Lean et al, 2006). Our results on the production of proinflammatory cytokines by C. parvum -treated DCs agrees with a recent work reporting production of the cytokines interleukin-12 p70, IL-2, IL-1beta and IL-6 by C. parvum -treated mice and human DCs (Bedi & Mead., 2012). …”

Section: Resultssupporting

confidence: 93%

Interaction ofCryptosporidium parvumwith mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes

Pérez-Cordón

Yang²,

Zhou³

et al. 2013

Pathogens Disease

View full text Add to dashboard Cite

Dendritic cells (DCs) are the antigen presenting cells capable of activating naïve T cells. Although CD4+ T cells are crucial for Cryptosporidium parvum clearance, little is known about the role of DCs in the immune response to this parasite. In this study the interaction between mouse DCs and C. parvum was investigated both in vitro and in vivo. For in vitro experiments, mouse bone marrow-derived dendritic cells (BMDCs) derived from wild type C57B1/6 or MyD88−/− or C3H/HeJ mice and DC cell line DC2.4 were pulsed with C. parvum. Active invasion of parasites was demonstrated by parasite co-localization with host cell membranes and actin-plaque formation at the site of attachment. DC activation induced by the parasite invasion was demonstrated by upregulation of costimulatory molecules CD40, CD80, and CD86, as well as inflammatory cytokines IL-12, TNF-α, and IL-6. BMDCs derived from MyD88−/− and C3H/HeJ mice failed to produce IL-12 in response to C. parvum, suggesting the importance of TLR-dependent signaling pathway specially presence of a functional TLR4 pathway, for C. parvum-induced cytokine production. In vivo experiments showed that both parasite antigens and live parasites were transported to mice mesenteric lymph nodes. All together, these data suggest that DCs play a key role in host immune responses to C. parvum and pathogenesis of the disease.

show abstract

Section: Resultssupporting

confidence: 93%

Interaction ofCryptosporidium parvumwith mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes

Pérez-Cordón

Yang²,

Zhou³

et al. 2013

Pathogens Disease

View full text Add to dashboard Cite

show abstract

“…Previously, a reduced number of eosinophils were found in the mucosa of piglets infected with Cryptosporidium parvum compared to control noninfected mucosa . Subjects with allergies infected with Cryptosporidium showed lower counts of eosinophils, lower percentages of CD4 + CD69 + and CD19 + cells, CD19 + CD23 + and CD19 + CD124 + cells, and lower levels of serum total IgE compared with noninfected allergic children, supporting a low Th2‐type IR and confirming the participation of a Th1‐type IR previously associated with Cryptosporidium . In our study, we found no relationship in the spectrum of allergic disease and the presence of Cryptosporidium , suggesting no relationship between the severity of allergic disease and Cryptosporidium infection.…”

Section: Discussionsupporting

confidence: 89%

Modulation of the immune response by infection with Cryptosporidium spp. in children with allergic diseases

Guangorena-Gómez

Maravilla-Domínguez

García‐Arenas

et al. 2016

Parasite Immunology

View full text Add to dashboard Cite

It has been demonstrated that the allergic response can be ameliorated by the administration of pathogen derivatives that activate Toll-like receptors and induce a Th1-type immune response (IR). Cryptosporidium is a parasite that promotes an IR via Toll-like receptors and elicits the production of Th1-type cytokines, which limit cryptosporidiosis. The aim of this study was to investigate allergy-related immune markers in children naturally infected with Cryptosporidium. In a cross-sectional study, 49 children with or without clinical diagnosis of allergies, oocysts of Cryptosporidium spp. in the faeces were screened microscopically. We microscopically screened for leucocytes, examined T and B cells for allergy-related activation markers using flow cytometry and evaluated serum for total IgE using chemiluminescence. Children with allergies and Cryptosporidium in the faeces had significantly lower levels of total IgE, B cells, CD19(+) CD23(+) and CD19(+) CD124(+) cells as well as a greater percentage of interferon-gamma (IFN-γ(+) ) and IL-4(+) CD4(+) cells than children with allergies without Cryptosporidium. This is the first description of the modulation of the IR in children with allergic diseases in the setting of natural Cryptosporidium infection. Our findings suggest the involvement of CD4(+) cells producing IL-4 and IFN-γ in the IR to Cryptosporidium in naturally infected children.

show abstract

“…These results were supported by studies done by Mead and colleagues, who found that IL-18 protected against C. parvum infection in vivo via stimulation of IFN-γ production and AMP expression (51)*. They also found that mouse dendritic cells were able to produce IL-18 upon stimulation with C. parvum antigens (56). It is unclear whether the effects of IL-18 on IFN-γ and AMP production are mutually exclusive, or whether IL-18-dependent stimulation of AMP production by IECs is due to enhanced IFN-γ secretion.…”

Section: Immune Responses To Cryptosporidiumsupporting

confidence: 60%

“…Dendritic cells migrate toward areas of C. parvum infection in an IFN- γ-dependent manner (38), but until recently, their role in clearance of the parasite was unclear. In vitro studies found that bone marrow-derived dendritic cells challenged with C. parvum sporozoites or antigens secreted a number of cytokines, including type I IFN, TNF-α, IL-6, IL-1β, IL-12, and IL-18 (36,56,59). More recent studies have elucidated the importance of dendritic cells in vivo .…”

Section: Immune Responses To Cryptosporidiummentioning

confidence: 99%

“…Antibodies targeting these proteins can inhibit Cryptosporidium infection in vitro (17,20,94), and humoral responses to several of these antigens are elevated in patients with cryptosporidiosis (25,84–89). A few of these antigens such as gp900, gp40, gp15/17 and Cp23/27 have been shown to induce cellular immune responses in animals or humans (56,80,95). However, it is not known whether these responses are protective.…”

Section: Vaccine Developmentmentioning

confidence: 99%

See 1 more Smart Citation

Systemic and Mucosal Immune Responses to Cryptosporidium—Vaccine Development

Ludington

Ward

2015

Curr Trop Med Rep

View full text Add to dashboard Cite

Cryptosporidium spp is a major cause of diarrheal disease worldwide, particularly in malnourished children and untreated AIDS patients in developing countries in whom it can cause severe, chronic and debilitating disease. Unfortunately, there is no consistently effective drug for these vulnerable populations and no vaccine, partly due to a limited understanding of both the parasite and the host immune response. In this review, we will discuss our current understanding of the systemic and mucosal immune responses to Cryptosporidium infection, discuss the feasibility of developing a Cryptosporidium vaccine and evaluate recent advances in Cryptosporidium vaccine development strategies

show abstract

Cryptosporidium parvum antigens induce mouse and human dendritic cells to generate Th1‐enhancing cytokines

Cited by 27 publications

References 51 publications

Interaction ofCryptosporidium parvumwith mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes

Interaction ofCryptosporidium parvumwith mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes

Modulation of the immune response by infection with Cryptosporidium spp. in children with allergic diseases

Systemic and Mucosal Immune Responses to Cryptosporidium—Vaccine Development

Contact Info

Product

Resources

About