<i>Cryptococcus festucosus</i> sp. nov. a new hymenomycetous yeast in the Holtermannia clade

The occurrence of culturable yeasts in glacial meltwater from the Frías, Castaño Overo and Río Manso glaciers, located on Mount Tronador in the Nahuel Huapi National Park (Northwestern Patagonia, Argentina) is presented. Subsurface water samples were filtered for colony counting and yeast isolation. The total yeast count ranged between 6 and 360 CFU L(-1). Physiologic and molecular methods were employed to identify 86 yeast isolates. In agreement with yeast diversity data from studies for Antarctic and Alpine glaciers, the genera Cryptococcus, Leucosporidiella, Dioszegia, Rhodotorula, Rhodosporidium, Mrakia, Sporobolomyces, Udeniomyces and Candida were found. Cryptococcus and Leucosporidiella accounted for 50% and 20% of the total number of strains, respectively. Among 21 identified yeast species, Cryptococcus sp. 1 and Leucosporidiella fragaria were the most frequent. The typically psychrophilic Mrakia yeast strain and three new yeast species, yet to be described, were also isolated. All yeast strains were able to grow at 5, 10, and 15 degrees C. Among yeast strains expressing extracellular enzymatic activity, higher proteolytic and lipolytic activities were obtained at 4 degrees C than at 20 degrees C.

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“…Cryptococcus festucosus was first isolated from leaves in the Moscow region, Russia (Golubev et al , 2004). The type strain of Cr.…”

Section: Resultsmentioning

confidence: 99%

Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina

García

Brizzio

Libkind

et al. 2007

FEMS Microbiology Ecology

138

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“… ¶ Fell & Phaff (1967), Spencer & Spencer (1997), Fell & Statzell‐Tallman (1998), Sláviková & Vadkertiová (2000), Poliakova et al (2001), Zhao et al (2002), Golubev et al (2003, 2004, 2006), Nakagawa et al (2004, 2005), Wuczkowski & Prillinger (2004), Bergauer et al (2005), Inácio et al (2005), Wuczkowski et al (2005), Nagahama (2006), Nakagawa et al (2006), Sperstad et al (2006), Kachalkin et al (2008), Shivaji et al (2008). …”

Section: Discussionmentioning

confidence: 99%

“…With the sole exception of C. santamariae , all species isolated from the Calderone Glacier have been observed previously in other cold habitats. In particular, 21 yeast and yeast‐like species were previously isolated from at least two habitats of glaciers worldwide, Antarctica and other cold environments (Table 6): A. pullulans, C. adeliensis, C. albidosimilis, C. festucosus, C. macerans, C. oeirensis, C. saitoi, C. stepposus, C. tephrensis, C. victoriae, C. wieringae, C. capitatum, D. crocea, M. intermedium, M. gelida, M. aquatica, M. cryoconiti, R. colostri, R. laryngis, R. psychrophenolica and S. roseus (Vishniac & Kurtzman, 1992; Deegenaars & Watson, 1998; Montes et al , 1999; Scorzetti et al , 2000; Sláviková & Vadkertiová, 2000; Thomas‐Hall et al , 2002; Vishniac, 2002; Birgisson et al , 2003; Libkind et al , 2003; Golubev et al , 2004, 2006; Nakagawa et al , 2004; Wuczkowski & Prillinger, 2004; Bergauer et al , 2005; Gilichinsky et al , 2005; Inácio et al , 2005; Arenz et al , 2006; Butinar et al , 2007; de García et al , 2007; Margesin et al , 2007a; Connell et al , 2008; Margesin & Fell, 2008; Turchetti et al , 2008; D. Libkind, pers. commun., 2009).…”

Section: Discussionmentioning

confidence: 99%

Yeast and yeast-like diversity in the southernmost glacier of Europe (Calderone Glacier, Apennines, Italy)

Branda

Turchetti

Diolaiuti

et al. 2010

FEMS Microbiology Ecology

129

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The present study reports the characterization of psychrophilic yeast and yeast-like diversity in cold habitats (superficial and deep sediments, ice cores and meltwaters) of the Calderone Glacier (Italy), which is the southernmost glacier in Europe. After incubation at 4 and 20 degrees C, sediments contained about 10(2)-10(3) CFU of yeasts g(-1). The number of viable yeast cells in ice and meltwaters was several orders of magnitude lower. The concomitant presence of viable bacteria and filamentous fungi has also been observed. In all, 257 yeast strains were isolated and identified by 26S rRNA gene D1/D2 and internal transcribed spacers (1 and 2) sequencing as belonging to 28 ascomycetous and basidiomycetous species of 11 genera (Candida, Cystofilobasidium, Cryptococcus, Dioszegia, Erythrobasidium, Guehomyces, Mastigobasidium, Mrakia, Mrakiella, Rhodotorula and Sporobolomyces). Among them, the species Cryptococcus gastricus accounted for almost 40% of the total isolates. In addition, 12 strains were identified as belonging to the yeast-like species Aureobasidium pullulans and Exophiala dermatitidis, whereas 15 strains, presumably belonging to new species, yet to be described, were also isolated. Results herein reported indicate that the Calderone Glacier, although currently considered a vanishing ice body due to the ongoing global-warming phenomenon, still harbors viable psychrophilic yeast populations. Differences of yeast and yeast-like diversity between the glacier under study and other worldwide cold habitats are also discussed.

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“…According to analysis of partial 26S rDNA sequences, Holtermannia corniformis and Holtermanniella species form a separate cluster (Scorzetti et al, 2002;Golubev et al, 2004), for which we propose the rank of order and the name Holtermanniales. As shown in Fig.…”

Section: The New Order Holtermannialesmentioning

confidence: 98%

Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade

Wuczkowski

Passoth

Turchetti

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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The novel genus Holtermanniella is proposed here to accommodate four Cryptococcus species closely related to Holtermannia corniformis that are included in the Holtermannia clade (Basidiomycota, Agaricomycotina). Thus, four novel combinations are proposed: Holtermanniella nyarrowii comb. nov., Holtermanniella festucosa comb. nov., Holtermanniella mycelialis comb. nov. and Holtermanniella wattica comb. nov. In addition, a novel anamorphic yeast species was studied with 15 isolates obtained from different habitats around the world. Analysis of the sequences of the D1/D2 region of their large subunit rDNA showed that the novel species is placed phylogenetically within the Holtermannia clade of the Tremellomycetes (Agaricomycotina, Basidiomycota). PCR fingerprinting and sequencing of ITS1–5.8S–ITS2 showed genetic intraspecific variability among the strains: three groups were formed, which did not correlate with geographical origin or substrate. This novel species, designated the type species of Holtermanniella gen. nov., is described as Holtermanniella takashimae sp. nov.; the type strain is CBS 11174T (=HB 982T =DBVPG 8012T). The order Holtermanniales ord. nov. is proposed here to include Holtermannia (the type genus) and Holtermanniella.

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Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade

Cited by 8 publications

References 7 publications

Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina

Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina

Yeast and yeast-like diversity in the southernmost glacier of Europe (Calderone Glacier, Apennines, Italy)

Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade

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