<i>Coxiella burnetii</i> Genotyping

Glazunova, Olga O.; Roux, Véronique; Freylikman, Olga; Sekeyová, Zuzana; Fournous, Ghislain; Tyczka, Judith; Токаревич, Н. К.; Kovacóvá, E; Marrie, Thomas J.; Raoult, Didier

doi:10.3201/eid1108.041354

Cited by 206 publications

(27 citation statements)

References 36 publications

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“…According to a study conducted by Polish scientists, MST61 shows the highest similarity to the MST20 genotype, as the MST61 genotype differs from MST20 by a single nucleotide deletion in the Cox37 intergenic region (Szymañska-Czerwiñska et al, 2019). Bacteria with the MST20 genotype contain the QpH1 plasmid (Glazunova et al, 2005). Elsewhere in the world, there are no data on the virulence of the MST61 strain.…”

Section: Discussionmentioning

confidence: 99%

“…Strongly positive samples (n = 26) with at least 5×10 4 genome equivalents / ml (Ct values < 27) were selected for genotyping and 10 intergenic spacers were used -Cox2, Cox5, Cox18, Cox20, Cox22, Cox37, Cox51, Cox56, Cox57, and Cox61, as described previously (Glazunova et al, 2005). The forward primer of Cox57 was modified as described previously (Di Domenico et al, 2014).…”

Section: Detection Of C Burnetii Genotype By Mstmentioning

confidence: 99%

“…For epidemiological reasons, genetic characterisation of C. burnetii is necessary (Sulyok et al, 2014). Using the multispacer sequence typing (MST) of genetically variable intergenic regions, initially 30 different allelic combinations were obtained (Glazunova et al, 2005). Currently there are more than 70 MST groups distinguished (https://ifr48.timone.univ-mrs.fr/mst/coxiella_burnetii/strains.html).…”

Section: Introductionmentioning

confidence: 99%

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Shedding of Coxiella Burnetii in Milk of Dairy Cattle and Evidence of Q Fever in Domestic Ruminants with Emphasis on Abortion Cases in Latvia

Šteingolde

Boikmanis

Laizāne

et al. 2022

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Q-fever is widespread globally. The goal of this study was to estimate the prevalence of Q-fever infection in Latvia among dairy cattle in 2018–2020, in comparison with that in 2012–2015. The shedding of Coxiella burnetii DNA in milk was assessed. Screening of blood samples of abortion cases for the presence of antibodies against Q-fever and testing of aborted fetuses for C. burnetii DNA were also carried out. Additionally, serum samples from clinically healthy cattle, sheep and goat were included. Overall, 18.34% of milk samples were positive, representing 11.02% of all tested sheds. In total, 20.62% of serum samples from the cattle that suffered abortions were positive or suspicious, representing 12.63% of all sheds. Only 3.33% of serum samples from clinically healthy cattle and 3.42% from sheep and goat were positive. The highest proportion of serologically positive and suspicious samples was observed in cattle that suffered abortion with age from three to nine years, and in the first and third gestation period. One dominant genotype of C. burnetii (MST61) was detected. Ruminant import from abroad was identified as a significant risk factor, as well as the dairy cattle population density.

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of C Burnetii Genotype By Mstmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Shedding of Coxiella Burnetii in Milk of Dairy Cattle and Evidence of Q Fever in Domestic Ruminants with Emphasis on Abortion Cases in Latvia

Šteingolde

Boikmanis

Laizāne

et al. 2022

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…MST analyses of C. burnetii were performed on selected samples using ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) ( Table 2 ). PCR conditions and primer sequences have been previously described [ 8 ]. Briefly, each amplification reaction contained 12.5 μL of TEMPase Hot Start 2x Master Mix BLUE (Ampliqon, Odense, Denmark), 0.5 μM of each primer, 5 μL of DNA sample (in the final concentration of 5 ng), and 5.5 µL of double-distilled water in a final reaction volume of 25 µL.…”

Section: Methodsmentioning

confidence: 99%

“…For C. burnetii genotyping, Glazunova et al selected ten spacers that had the most variations. This technique was able to classify 173 isolates of C. burnetii into 30 genotypes [ 8 ]. Due to its high-resolution power, this technique allows direct comparison of results between different laboratories.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

et al. 2022

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Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian domestic ruminants. Understanding the C. burnetii genotypic profiles is critical in determining and preventing Q fever outbreaks.

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Disorders of Sheep and Goats

Moeller

2011

Kirkbride's Diagnosis of Abortion and Neonatal Loss in Animals

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Coxiella burnetii Genotyping

Cited by 206 publications

References 36 publications

Shedding of Coxiella Burnetii in Milk of Dairy Cattle and Evidence of Q Fever in Domestic Ruminants with Emphasis on Abortion Cases in Latvia

Shedding of Coxiella Burnetii in Milk of Dairy Cattle and Evidence of Q Fever in Domestic Ruminants with Emphasis on Abortion Cases in Latvia

Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

Disorders of Sheep and Goats

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