Corynebacterium cutisLysate Treatment Can Increase the Efficacies of PPR Vaccine

Abstract

This study aimed to evaluate the effects of Peste des petits ruminants (PPR) vaccine on cytokine and antibody levels in sheep when administered alone or in combination with Corynebacterium cutis lysate (CCL). The PPR vaccine group received a single subcutaneous axillary injection of the PPR vaccine (1 mL containing tissue culture infectious dose (TCID) attenuated live PPRV, n = 6) and the combination treatment (1 mL CCL and 1 mL PPR vaccine, n = 6) groups received a single subcutaneous axillary injection of both CCL and PPR vaccine. Blood samples were collected from sheep before the treatment and at different points after treatment (1, 3, 7, 14, 21, and 28 days). Plasma and serum samples were evaluated for antibody percentage, levels of cytokines IL-6, IL-10, IFN-γ, IL-4, IL-12, and IL-18, oxidative stress marker Thiobarbituric acid reactive substances, and hematological and biochemical parameters. Maximum protective antibody levels reach 3-4 weeks after vaccine administration. The combination treatment resulted in significant changes in IFN-γ, IL-4, IL-12, and IL-18 cytokine levels. These changes were not evident when only the PPR vaccine was administered and antibody percentage against PPRV was short term in PPR vaccine group. In conclusion, combined usage of the PPR vaccine with CCL resulted in a heightened cytokine response, leading to improved antibody level against PPR virus. Repeated CCL treatments can lead to earlier vaccine potency, provide protective efficacy for a longer time, and increase passive immunity.

Effect of Some Immunomodulators Use Together with PPR Vaccine on Immune system in Morkaraman Sheep

“…Er et al (2015 ) found that CCL administration did not cause a significant change in hematological parameters in sheep. Dik et al (2016) determined that the administration of CCL showed a decrease in the numbers of WBC and LYM in sheep. This study is accordance with the study conducted by Er et al (2015) by similarly concluding that there was no statistically significant difference when animals within the Group E based on days were compared, the comparison of the Group E with the Group F was performed and the overall comparison of group-based general evaluations of hematological parameters was taken into consideration (P>0.05).…”

“…This study is accordance with the study conducted by Er et al (2015) by similarly concluding that there was no statistically significant difference when animals within the Group E based on days were compared, the comparison of the Group E with the Group F was performed and the overall comparison of group-based general evaluations of hematological parameters was taken into consideration (P>0.05). Yılmaz et al (2011) argued that the use of CCL led to an increase in the level of IgG in the colostrum and serum of sheep and in newborns, Dik et al (2016) pointed out that the protective immune response continued throughout the study process in sheep which received combined treatment (CCL and PPR vaccine) although the peak level of Abs in the group started 7 days after administration, In addition, Saat et al (2016) stated that the IgG ratio in the milk of cattle with mastitis increased significantly after the admin-5580 Ö. AYDIN, M.S. AKTAŞ istration.…”

“…5575 Group E (CCL group, n = 8): 1 ml/ sheep of CCL (Ultracorn ® , Virbac, France) containing 20 mg CCL in 1 mL was subcutaneously administered to the sheep in this group as well as PPR vaccine (Dik et al, 2016).…”

Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats

“…The undesirable effects of drugs or diseases in organs or systems can be determined by biochemical and hematological parameters [ 1 , 13 ]. Free oxygen radicals produce by various agents such as LPS cause oxidative stress and cellular damage.…”

“…Free oxygen radicals produce by various agents such as LPS cause oxidative stress and cellular damage. Thiobarbituric acid reactive substances (TBARS) are detected in the cellular damage [ 13 , 47 ]. The underlying cause of oxidative stress in endotoxemia is cell damage and excessive TNF-α levels [ 38 ].…”