<i>comCDE</i>
            (Competence) Operon Is Regulated by CcpA in Streptococcus pneumoniae D39

Zhang, Yapeng; Zhang, Jinghui; Xiao, Jiangming; Wang, Hanyi; Yang, Rui; Guo, Xinlin; Zheng, Yan; Yin, Yibing; Zhang, Xuemei

doi:10.1128/spectrum.00012-23

Cited by 7 publications

(4 citation statements)

References 87 publications

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“…Phosphorylated ComE recognizes conserved promoter site called a ComE-binding site (Ceb) to control transcription, and this element is found upstream of the comAB and comCDE operons, meaning that CSP-mediated signaling can further activate the ComCDE system ( Ween et al., 1999 ). In addition, recent evidence indicates that the transcription of comCDE in S. pneumoniae is influenced by catabolite control protein A (CcpA), indicating that available carbon sources can also contribute to signaling through this system ( Zhang et al., 2023 ).…”

Section: Streptococcal Signaling Peptidesmentioning

confidence: 99%

Streptococcal peptides and their roles in host-microbe interactions

Wahlenmayer,

Hammers

2023

Front. Cell. Infect. Microbiol.

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The genus Streptococcus encompasses many bacterial species that are associated with hosts, ranging from asymptomatic colonizers and commensals to pathogens with a significant global health burden. Streptococci produce numerous factors that enable them to occupy their host-associated niches, many of which alter their host environment to the benefit of the bacteria. The ability to manipulate host immune systems to either evade detection and clearance or induce a hyperinflammatory state influences whether bacteria are able to survive and persist in a given environment, while also influencing the propensity of the bacteria to cause disease. Several bacterial factors that contribute to this inter-species interaction have been identified. Recently, small peptides have become increasingly appreciated as factors that contribute to Streptococcal relationships with their hosts. Peptides are utilized by streptococci to modulate their host environment in several ways, including by directly interacting with host factors to disrupt immune system function and signaling to other bacteria to control the expression of genes that contribute to immune modulation. In this review, we discuss the many contributions of Streptococcal peptides in terms of their ability to contribute to pathogenesis and disruption of host immunity. This discussion will highlight the importance of continuing to elucidate the functions of these Streptococcal peptides and pursuing the identification of new peptides that contribute to modulation of host environments. Developing a greater understanding of how bacteria interact with their hosts has the potential to enable the development of techniques to inhibit these peptides as therapeutic approaches against Streptococcal infections.

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Section: Streptococcal Signaling Peptidesmentioning

confidence: 99%

Streptococcal peptides and their roles in host-microbe interactions

Wahlenmayer,

Hammers

2023

Front. Cell. Infect. Microbiol.

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“…This issue is closely linked to genetic transformation that is employed by pneumococci for acquiring antibiotic resistance, adapting to their environment, and even generating novel species ( Straume et al., 2015 ; Kim et al., 2019 ; Lo et al., 2019 ). Consequently, there has been an increasing interest in investigating the mechanisms underlying gene transfer ( Vollmer et al., 2019 ; Lam et al., 2021 ; Zhang et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of TacL-mediated lipoteichoic acids biosynthesis by ComE during competence impacts pneumococcal transformation

Yao,

Wang,

Song

et al. 2024

Front. Cell. Infect. Microbiol.

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Competence development is essential for bacterial transformation since it enables bacteria to take up free DNA from the surrounding environment. The regulation of teichoic acid biosynthesis is tightly controlled during pneumococcal competence; however, the mechanism governing this regulation and its impact on transformation remains poorly understood. We demonstrated that a defect in lipoteichoic acid ligase (TacL)-mediated lipoteichoic acids (LTAs) biosynthesis was associated with impaired pneumococcal transformation. Using a fragment of tacL regulatory probe as bait in a DNA pulldown assay, we successfully identified several regulatory proteins, including ComE. Electrophoretic mobility shift assays revealed that phosphomimetic ComE, but not wild-type ComE, exhibited specific binding to the probe. DNase I footprinting assays revealed the specific binding sequences encompassing around 30 base pairs located 31 base pairs upstream from the start codon of tacL. Expression of tacL was found to be upregulated in the ΔcomE strain, and the addition of exogenous competence-stimulating peptide repressed the tacL transcription in the wild-type strain but not the ΔcomE mutant, indicating that ComE exerted a negative regulatory effect on the transcription of tacL. Mutation in the JH2 region of tacL upstream regulatory sequence led to increased LTAs abundance and displayed higher transformation efficiency. Collectively, our work identified the regulatory mechanisms that control LTAs biosynthesis during competence and thereby unveiled a repression mechanism underlying pneumococcal transformation.

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“…In the growing insect industry, entomopathogenic bacteria can quickly and easily infect and multiply, resulting in the reduced production of industrial insects and subsequent economic losses [16]. Sepsis, a common bacterial disease in insects, is associated with entomopathogenic bacteria that attach to specific tissue surfaces and multiply, producing various immunosuppressive factors, toxin proteins, and specialized metabolites that cause rapid death of the host [17]. Therefore, entomopathogenic bacteria are often used as biological control agents to suppress pests in plant cultivation.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, entomopathogenic bacteria are often used as biological control agents to suppress pests in plant cultivation. Representative entomopathogenic bacteria include Bacillaceae, Pseudomonadaceae, Enterobacteriaceae, Streptococcaceae, Micrococcaceae, and Paeni-bacillaceae; additionally, S. marcescens and S. entomophila are attracting attention for use as biological control agents owing to their strong pathogenicity [16,17]. S. marcescens and S. entomophila infect lepidopteran insects, impairing their feeding and growth, and rapidly proliferate and secrete large amounts of proteolytic enzymes such as β-hemolysins, elastases, and chitinases.…”

Section: Introductionmentioning

confidence: 99%

Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp.

Park,

Jeong

et al. 2024

IJMS

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Diseases that occur in silkworms include soft rot, hardening disease, digestive diseases, and sepsis. However, research on the causes of bacterial diseases occurring in silkworms and the resulting changes in the microbial community is lacking. Therefore, we examined the morphological characteristics of sepsis and changes in the microbial community between silkworms that exhibit a unique odor and healthy silkworms; thus, we established a relationship between disease-causing microorganisms and sepsis. After producing a 16S rRNA amplicon library for samples showing sepsis, we obtained information on the microbial community present in silkworms using next-generation sequencing. Compared to that in healthy silkworms, in silkworms with sepsis, the abundance of the Firmicutes phylum was significantly reduced, while that of Proteobacteria was increased. Serratia sp. was dominant in silkworms with sepsis. After bacterial isolation, identification, and reinfection through the oral cavity, we confirmed this organism as the disease-causing agent; its mortality rate was 1.8 times higher than that caused by Serratia marcescens. In summary, we identified a new causative bacterium of silkworm sepsis through microbial community analysis and confirmed that the microbial community balance was disrupted by the aberrant proliferation of certain bacteria.

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comCDE (Competence) Operon Is Regulated by CcpA in Streptococcus pneumoniae D39

Abstract: Streptococcus pneumoniae is a major cause of bacterial infections in humans, such as pneumonia, bacteremia, meningitis, otitis media, and sinusitis. Like most streptococci, S. pneumoniae is naturally competent and employs this ability to augment its adaptive evolution.

Cited by 7 publications

References 87 publications

Streptococcal peptides and their roles in host-microbe interactions

Streptococcal peptides and their roles in host-microbe interactions

Transcriptional regulation of TacL-mediated lipoteichoic acids biosynthesis by ComE during competence impacts pneumococcal transformation

Microbial Community Changes in Silkworms Suspected of Septicemia and Identification of Serratia sp.

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