<i>Colletotrichum gloeosporioides,</i>a Possible Biological Control Agent for<i>Clidemia hirta</i>in Hawaiian Forests

Trujillo, E. E.; Latterell, Frances M.; Rossi, A. E.

doi:10.1094/pd-70-974

Cited by 26 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strains of Colletotrichum malvarnum and Colletotrichum coccodes which show promise as biocontrol agents against prickly sida (Sida spinosa) and velvetleaf (Abutilon theophrasti), respectively, have been identified (4,9). Strains of C. gloeosporioides which specifically infect the noxious weeds Clidemia hirta and Malva pussilla have been discovered (5,8).…”

mentioning

confidence: 99%

Carbon Concentration and Carbon-to-Nitrogen Ratio Influence Submerged-Culture Conidiation by the Potential Bioherbicide Colletotrichum truncatum NRRL 13737

Jackson

Bothast

1990

Appl Environ Microbiol

View full text Add to dashboard Cite

We assessed the influence of various carbon concentrations and carbon-to-nitrogen (C:N) ratios on Colletotrichum truncatum NRRL 13737 conidium formation in submerged cultures grown in a basal salts medium containing various amounts of glucose and Casamino Acids. Under the nutritional conditions tested, the highest conidium concentrations were produced in media with carbon concentrations of 4.0 to 15.3 g/liter. High carbon concentrations (20.4 to 40.8 g/liter) inhibited sporulation and enhanced the formation of microsclerotiumlike hyphal masses. At all the carbon concentrations tested, a culture grown in a medium with a C:N ratio of 15:1 produced more conidia than cultures grown in media with C:N ratios of 40:1 or 5:1. While glucose exhaustion was often coincident with conidium formation, cultures containing residual glucose sporulated and those with high carbon concentrations (>25 g/liter) exhausted glucose without sporulation. Nitrogen source studies showed that the levels of C. truncatum NRRL 13737 conidiation were similar for all protein hydrolysates tested. Reduced conidiation occurred when amino acid and inorganic nitrogen sources were used. Of the nine carbon sources evaluated, acetate as the sole carbon source resulted in the lowest level of sporulation.

show abstract

mentioning

confidence: 99%

Carbon Concentration and Carbon-to-Nitrogen Ratio Influence Submerged-Culture Conidiation by the Potential Bioherbicide Colletotrichum truncatum NRRL 13737

Jackson

Bothast

1990

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Strains of this fungus have been patented (J. T. Daniel, G. E. Templeton, and R. J. Smith, Jr., U.S. patent 3,849,104, Nov. 1974) for the control of northern jointvetch (Aeschynomene virginica) and winged waterprimrose (Jussiaea decurrens), serious weeds in soybean and rice fields, respectively. More recently, C. gloeosporioides has been suggested as a possible biological control agent for Clidemia hirta, an invading weed in Hawaiian forests (29).…”

mentioning

confidence: 99%

Spore Yield and Microcycle Conidiation of Colletotrichum gloeosporioides in Liquid Culture

Cascino

Harris

Smith

et al. 1990

Appl Environ Microbiol

View full text Add to dashboard Cite

The effect of V8 juice concentration (5 to 40%, vol/vol), spore inoculum density (105 and 107 spores per ml), and liquid batch or fed-batch culture condition on mycelium and spore production by Colletotrichum gloeosporioides was evaluated. The amount of mycelium produced, the time required for initiation of sporulation following attainment of maximum tnycelium, and the time for attainment of maximum spore concentration increased with increasing V8 juice concentration in batch culture. Cultures containing V8 juice at >10% achieved a similar spore density (apparent spore-carrying capacity) of about 0.8 mg of spores per ml (1 X 107 to 2 x 107 spores per ml) independent of inoculum density and V8 juice concentration. The relative spore yield decreased from a high of 64% of the total biomass for the low-inoculum 5% V8 culture, through 13% for the analogous 40% V8 culture, to a low of 2% for the high-inoculum 27% V8 culture. Fed-batch cultures were used to establish conditions of high spore density and low substrate availability but high substrate flux. The rate of addition of V8 juice was adjusted to approximate the rate of substrate utilization by the (increasing) biomass. The final spore concentration was about four times higher (3.0 mg of spores per ml) than the apparent spore-carrying capacity in batch culture. This high spore yield was obtained at the expense of greatly reduced mycelium, resulting in a high relative spore yield (62% of the total biomass). Microcycle conidiation occurred in the fed-batch but not batch systems. These data indicate that substrate-limited, fed-batch culture can be used to increase the amount and efficiency of spore production by C. gloeosporioides by maintaining microcycle conidiation conditions favoring allocation of nutrients to spore rather than mycelium production.

show abstract

“…Thèse results, combined with the results from the whole plant comparison, were the basis for continued évaluation of C. dematium. The sélection of C. dematium was also supported by the demonstrated utility of Colletotrichum species in other inundative biocontrol research programs (Anderson and Walker 1985;Boyette 1991;Boyette et al 1979;Hildebrand and Jensen 1991;McRae and Stevens 1990;Mortensen 1988;Smith 1986;Trujillo et al 1986;Wymore et al 1988).…”

Section: Inoculum Productionmentioning

confidence: 86%

A potential microbial control for fireweed (Epilobium angustifolium)

Winder¹

2005

phyto

View full text Add to dashboard Cite

La champignon a été pathogène pour l'épilobe à feuilles étroites et l'E. lanceolatum, alors que les autres espèces évaluées ont été résistantes ou immunisées. Les dimensions des conidies et des appressoria et le nombre restreint de plantes-hôtes supportent l'hypothèse que cet isolât pourrait être une forme-espèce non répertoriée. L'application d'une formulation de conidies âgées de 10 à 15 j, à une concentration de 10 conidies m et après une période de rosée de 18 à 24 h, a causé la mortalité de 100% des rosettes d'épilobe à feuilles étroites âgées de 7 semaines dans les 48 h suivant l'application. La virulence a été réduite chez les plantes plus âgées. Dans l'essai au champ, la croissance des rosettes infectées a diminué de 33%. Ces résultats démontrent le potentiel de C. dematium comme agent de lutte biologique en sylviculture contre les plantules d'épilobe à feuilles étroites. 1993-04-15; acceptée 1993-12-24 Diseased fireweed {Epilobium angustifolium) plants were collected in Québec and organisms isolated from thèse plants were evaluated as potential biocontrol agents. Thirteen pathogenic fungi were isolated and three of thèse {Colletotrichum dematium, Seimatosporium kriegerianum, and Alternaria alternata) were virulent in initial screening assays. C. dematium was selected for further study because it was the most virulent pathogen, causing large necrotic lésions on leaves and stems of infected plants. Inoculum production was optimized on modified malt extract agar and the virulence enhanced by suppression of the conidial matrix with tannic acid and the addition of extracts of Aloe saponaria. The fungus was pathogenic to fireweed and E. lanceolatum, while other test species were very résistant or immune. Measurements of conidial and appressorial dimensions and its restricted host range support the hypothesis that the isolate may be an unreported form-species. Application of formulated conidia consistently provided 100% mortality of 7-wk-old inoculated fireweed rosettes within 48 h using 10 9 conidia m 2 , from 10-to 15-d-old inoculum, and a 18-to 24-h dew period. Virulence was diminished in older plants. In field trials, growth of inoculated rosettes was reduced by 33%. Thèse results suggest that C. dematium is a promising candidate for further development as a control agent for seedling fireweed in silviculture.

show abstract

Colletotrichum gloeosporioides,a Possible Biological Control Agent forClidemia hirtain Hawaiian Forests

Cited by 26 publications

References 0 publications

Carbon Concentration and Carbon-to-Nitrogen Ratio Influence Submerged-Culture Conidiation by the Potential Bioherbicide Colletotrichum truncatum NRRL 13737

Carbon Concentration and Carbon-to-Nitrogen Ratio Influence Submerged-Culture Conidiation by the Potential Bioherbicide Colletotrichum truncatum NRRL 13737

Spore Yield and Microcycle Conidiation of Colletotrichum gloeosporioides in Liquid Culture

A potential microbial control for fireweed (Epilobium angustifolium)

Contact Info

Product

Resources

About