COI1 : An Arabidopsis Gene Required for Jasmonate-Regulated Defense and Fertility

Abstract

The coi1 mutation defines an Arabidopsis gene required for response to jasmonates, which regulate defense against insects and pathogens, wound healing, and pollen fertility. The wild-type allele, COI1, was mapped to a 90-kilobase genomic fragment and located by complementation of coi1-1 mutants. The predicted amino acid sequence of the COI1 protein contains 16 leucine-rich repeats and an F-box motif. It has similarity to the F-box proteins Arabidopsis TIR1, human Skp2, and yeast Grr1, which appear to function by targeting repressor proteins for removal by ubiquitination.

High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

“…In addition the sequence of the ps-2 gene will give us more insights into the physiology of anther dehiscence in tomato. Similar phenotypes have been observed in some Arabidopsis mutants where, in most of the cases, the jasmonic acid pathway was involved (Feys et al 1994;Ishiguro et al 2001;von Malek et al 2002;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;Xie et al 1998). Once the ps-2 gene is cloned, it will be interesting to know whether this gene is ortholog to one of those genes already identiWed in Arabidopsis.…”

“…The molecular mechanism of ps-2 in tomato is unknown, but similar mutant phenotypes have been observed and studied mainly in Arabidopsis, such as the mutant myb26 (Steiner-Lange et al 2003), the mutants coi1 (Feys et al 1994;Xie et al 1998) and dad1 (Ishiguro et al 2001), the double mutants opr3/ dde1 (Sanders et al 2000;Stintzi and Browse 2000) and aos/dde2-2 (von Malek et al 2002;Park et al 2002) and the triple mutant fad3/fad7/fad8 (McConn and Browse 1996). They are characterized by defects in Wlament elongation, timing of anther dehiscence and often show reduced pollen viability.…”

“…All, except the mutant myb26, are in one way or another involved in the jasmonic acid pathway. The mutant coi1 (Xie et al 1998) is insensitive to jasmonic acid, while the others are defective in jasmonic acid synthesis (Feys et al 1994;Ishiguro et al 2001;McConn and Browse 1996;Park et al 2002;Sanders et al 2000;Stintzi and Browse 2000;von Malek et al 2002). Atanassova (1999) observed up to 26% self-pollination in the most extreme cases, on cultivar "Vrbicanske nizke" (ps-2/ps-2) under Weld conditions.…”

Sugar-Hormone Cross-Talk in Anthocyanin Biosynthesis

“…The F-box protein Coronatine Insensitive-1 (COI1), which forms the SCF COI1 complex with ASK1/ASK2, Cullin1, and Rbx1, is involved in diverse JA responses (Xu et al, 2002), most of which are disrupted in the Arabidopsis mutant coronatine insensitive 1 (coi1). A coi1-1 mutant with a premature stop codon at W467 failed to accumulate anthocyanin in response to methyl JA (Reymond and Farmer, 1998;Xie et al, 1998). Analysis of several transcript levels in coi1-1 mutants showed reduced expression of three anthocyanin regulatory factors, PAP1, PAP2 and GL3, which transcriptionally regulate the LBGs DFR, LDOX and UF3GT (Devoto et al, 2005).…”

Ubiquitination-mediated protein degradation and modification: an emerging theme in plant-microbe interactions

“…Among the members of the F-box-type E3s implicated in plant defense signaling, the Coronatine Insensitive1 (COI1) gene is the best example from which detailed functional characterization is available. COI1 encodes a 66-kDa protein with amino-terminal F-box motif and a leucine-rich repeats (LRR) domain close to the carboxy-terminal end [47]. COI1 is required for plant response to jasmonic acids (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens [48,49].…”