<i>Cochliobolus</i> phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences

Berbee, Mary L.; Pirseyedi, Mona; Hubbard, Sheryl

doi:10.1080/00275514.1999.12061106

Cited by 377 publications

(114 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cochliobolus has Bipolaris and Curvularia anamorphs, and results obtained from 28S rDNA analyses confirm a close phylogenetic connection between these asexual and sexual fungi. Phylogenies also corroborate find- ings reported by Berbee et al (1999) that Cochliobolus species can be divided broadly into two groups. With the inclusion of different taxa in this study we found that C. pallescens (5Curvularia pallescens), Bipolaris papendorfii, C. lunatus (5Curvularia lunatus), C. hawaiiensis (5Bipolaris hawaiiensis) and Curvularia heteropogonicola represent a distinct monophyletic group (similar to Group 2 as defined by Berbee et al 1999).…”

Section: Discussionsupporting

confidence: 87%

“…Phylogenetics of Cochliobolus, Pleospora and Setosphaeria (Clade A 1 ).-Several phylogenetic studies within the loculoascomycetes have included Pleospora as a typical representative of the Pleosporales, which is defined by the presence of cellular pseudoparaphyses (Berbee 1996, Dong et al 1998, Silva-Hanlin and Hanlin 1999, Lumbsch 2000, Lumbsch and Lindemuth 2001, Lumbsch et al 2001. Cochliobolus, Pleospora and Setosphaeria currently are accepted genera within the family Pleosporaceae (Sivanesan 1984, Eriksson and Hawksworth 1998, Kirk et al 2001, Eriksson 2005.…”

Section: Discussionmentioning

confidence: 99%

“…Barr (1987b) redefined the Pleosporaceae to include Clathrospora (5Comoclathris), Kirschsteiniothelia, Lewia and Pleospora and grouped Cochliobolus, Pyrenophora and Setosphaeria into the family Pyrenophoraceae. Berbee (1996) disagreed, suggesting that all those genera belong to the Pleosporaceae.…”

Section: Introductionmentioning

confidence: 91%

“…Their anamorphs are usually hyphomycetes and also have been reported to cause plant disease in cereals (e.g. Bipolaris maydis, Exserohilum turcicum and Helminthosporium oryzae) (Farr et al 1989, Berbee 1996, Krupinsky et al 2004, Agrios 2005.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The family Pleosporaceae: intergeneric relationships and phylogenetic perspectives based on sequence analyses of partial 28S rDNA

et al. 2006

View full text Add to dashboard Cite

The Pleosporaceae is an important loculoascomycete family. There has been disagreement, however, regarding the taxonomic placement of many genera within this family. This study investigates phylogenetic relationships among the genera Cochliobolus, Kirschsteiniothelia, Leptosphaerulina, Macroventuria, Pleospora, Pyrenophora, and Wettsteinina. Partial 28S rDNA sequences from taxa within these genera were analyzed with maximum parsimony, likelihood and Bayesian methods. Cochliobolus can be segregated broadly into two groups as previously proposed. Pleospora is polyphyletic in its current sense. Taxa with Stemphylium anamorphs are closely related to Cochliobolus and fit within the Pleosporaceae, whereas the affinities of Pleospora herbarum and P. ambigua are still ambiguous. Pyrenophora constitutes a monophyletic group within the Pleosporaceae, whereas Leptosphaerulina and Macroventuria appear to share phylogenetic affinities with the Leptosphaeriaceae and Phaeosphaeriaceae. Phylogenies indicate that Wettsteinina should be excluded from the Pleosporaceae. Similar findings are reported for Kirschsteiniothelia, which is probably polyphyletic. Anamorphic characters appear to be significant (especially in Cochliobolus) while ascospore morphologies, such as shape and color and substrate occurrence are poor indicators of phylogenetic relationships among these loculoascomycetes.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The family Pleosporaceae: intergeneric relationships and phylogenetic perspectives based on sequence analyses of partial 28S rDNA

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Amplification of ITS and mtSSU rDNA was conducted according to previously described protocols using respectively primer pairs ITS5/ ITS4 (White et al 1990) and NMS1/NMS2 (Li et al 1994) (Pryor and Gilbertson 2002). Amplification of protein coding genes actin (ACT), Alt a 1, b-tubulin, chitin synthase (CHS), EF-1a, and gpd was accomplished with primers pairs ACT-512F/ACT-783R (Carbone and Kohn 1999), Alt-for/ Alt-rev (Hong et al 2005), Bt1a/Bt1b (Glass and Donaldson 1995), CHS-79F/CHS-354R (Carbone and Kohn 1999), EF1-728F/EF1-986R (Carbone 1999), and gpd1/gpd2 (Berbee et al 1999) respectively. Each PCR mixture contained 5 pmol of each primers, 200 mM dNTP, 13 Taq reation buffer, 2 unit of AmpliTaq-DNA polymerase, 2.5 mM MgCl 2 and 10 ng of template DNA in a final volume of 25 mL.…”

Section: Methodsmentioning

confidence: 99%

A re-examination of the phylogenetic relationship between the causal agents of carrot black rot,Alternaria radicinaandA. carotiincultae

Park¹,

Romanoski²,

Pryor

2008

Mycologia

View full text Add to dashboard Cite

The phylogenetic relationship between Alternaria radicina and A. carotiincultae was reexamined based on morphology, sequence analysis of rDNA (ITS and mitochondrial small subunit [mtSSU]), protein coding genes (actin [ACT], beta-tubulin, chitin synthase [CHS], translation elongation factor [EF-1a], Alternaria allergen a1 [Alt a1], and glyceraldehyde-3-phosphate dehydrogenase [gpd]), and RAPD and ISSR analysis of total genomic DNA. Although some morphological characters overlapped to a degree, with A. radicina isolates expressing moderate variation and A. carotiincultae isolates being highly uniform, A. carotiincultae could be differentiated from A. radicina based on significantly greater growth rate on potato dextrose agar (PDA) or acidified PDA (APDA) and average number of transverse septa per conidium. Sequence of rDNA and two protein coding genes, ACT and CHS, were invariant between species. However polymorphism with the EF-1a, beta-tubulin, and Alt a1 gene strictly separated the population of A. radicina and A. carotiincultae as distinct lineages, as did RAPD and ISSR analysis. The polymorphic gpd gene did not strictly separate the two species. However isolates of A. radicina encompassed several haplotypes, one of which was the exclusive haplotype possessed by A. carotiincultae isolates, suggesting evidence of incomplete lineage sorting. The results suggest that A. carotiincultae is closely related to A. radicina but is a recently divergent and distinct lineage, which supports its status as a separate species.

show abstract

Phylogenetic evaluation of Bipolaris and Curvularia species collected from turfgrasses

Connally

Smith

Marek

et al. 2021

Intl Turfgrass Soc Res J

View full text Add to dashboard Cite

The dothideomycete genera Bipolaris and Curvularia are important pathogens of grasses (Poaceae) and can associate with host tissues without disease symptoms.Species of both genera are responsible for leaf spot epidemics that commonly occur on turfgrass throughout the United States transition zone and southern regions.Recent species typifications with DNA sequences have made a broad examination of Bipolaris and Curvularia species obtained from turfgrass possible. Isolates for analysis were obtained from culture collections or isolated from symptomatic turfgrass samples. Phylogenies based on ITS, GPD, and TEF gene sequences were constructed using maximum likelihood and Bayesian analyses and used to determine intraspecific and intrageneric relationships among Bipolaris and Curvularia species isolated from turfgrasses. Similar to previous studies, Bipolaris and Curvularia species formed separate, well-supported clades. Morphological identifications of genera and species based on published host-based keys often disagreed with molecular groupings. For example, C. hawaiiensis (= B. hawaiiensis) could not be differentiated from B. cynodontis while C. australiensis (= B. australiensis) could not be differentiated from C. spicifera (= B. spicifera). Additionally, phylogenies suggest B. coffeana and B. cynodontis are synonymous. However, the number of pseudosepta per conidium was the only consistent morphological character. The delineation of species causing leaf spot diseases of warm-season turfgrasses should improve disease management decisions and help identify potential pathogens in asymptomatic tissues.

show abstract

Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences

Cited by 377 publications

References 18 publications

The family Pleosporaceae: intergeneric relationships and phylogenetic perspectives based on sequence analyses of partial 28S rDNA

The family Pleosporaceae: intergeneric relationships and phylogenetic perspectives based on sequence analyses of partial 28S rDNA

A re-examination of the phylogenetic relationship between the causal agents of carrot black rot,Alternaria radicinaandA. carotiincultae

Phylogenetic evaluation of Bipolaris and Curvularia species collected from turfgrasses

Contact Info

Product

Resources

About