<i>Clostridium tyrobutyricum</i> alleviates <i>Staphylococcus aureus</i>-induced endometritis in mice by inhibiting endometrial barrier disruption and inflammatory response

Hu, Xiaoyu; Guo, Jian; Xu, Mingyue; Jiang, Peng; Yuan, Xin; Zhao, Caijun; Maimai, T.; Cao, Yongguo; Zhang, Naisheng; Fu, Yunhe

doi:10.1039/c9fo00654k

Cited by 31 publications

(24 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[24][25][26] Ct, a Gram-positive bacterium, has been shown to prevent LPS-induced intestinal barrier dysfunction in vitro and alleviate Staphylococcus aureus-induced endometrial inflammation in vivo. [27,28] However, whether Ct could alleviate intestinal inflammation is still unclear. In this study, we found that 10 7 CFU mL −1 Ct could alleviate inflammation in the duodenum and 10 8 CFU mL −1 Ct protected against duodenal and ileal inflammation, indicating that Ct was a potential prophylactic probiotic in the gut.…”

Section: Discussionmentioning

confidence: 99%

A Potential Prophylactic Probiotic for Inflammatory Bowel Disease: The Overall Investigation of Clostridium tyrobutyricum ATCC25755 Attenuates LPS‐Induced Inflammation via Regulating Intestinal Immune Cells

Zhang

Liu

Jin

et al. 2021

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope: This study aims to roundly investigate whether Clostridium tyrobutyricum (Ct) alleviates inflammation via regulating immune cells in the small intestines. Methods and Results: Mice are pre-treated with different concentrations of Ct follow by LPS stimulation. Ct maintains the mice body weight under inflammation. In response to LPS, 10 7 CFU mL −1 Ct decreases the mRNA expression of inflammatory cytokines in the duodenum, while 10 8 CFU mL −1 Ct reduces inflammatory cytokines expression in both duodenum and ileum and protected intestinal morphology. The small intestinal immune cells are analyzed using flow cytometry. Ct decreases the numbers of macrophages and mast cells in the intestines in response to LPS. In the duodenum, Ct enhances dentritic cells (DCs), regulatory T cells (Tregs), and T helper cell 17 (Th17) proportions. Ct decreases DCs and Tregs proportions, while enhances Th17 numbers in the ileum. The underlying mechanism of Ct in preventing inflammation may rely on the physiological immune cell composition of the intestines. In response to LPS, Ct may mainly stimulate Tregs via activating DCs in the duodenum while trigger Th17 cells in the ileum, thereby maintaining the intestinal homeostasis. Conclusion: Ct alleviates the LPS-induce inflammation via regulating different immune cell types in the small intestines, highlighting that Ct is a potential prophylactic probiotic in intestinal diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

A Potential Prophylactic Probiotic for Inflammatory Bowel Disease: The Overall Investigation of Clostridium tyrobutyricum ATCC25755 Attenuates LPS‐Induced Inflammation via Regulating Intestinal Immune Cells

Zhang

Liu

Jin

et al. 2021

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…When inflammation occurs, the IκB kinase triggers IκB-α ubiquitination and proteasome degradation via phosphorylation, which allows NF-κB p65 to accumulate in the nucleus to elicit the production of cytokines, such as TNF-α, IL-1β, and IL-6 ( 42 ). Previous studies have shown that S. aureus infection can increase the phosphorylation of IκB-α and p65 proteins, and activate NF-κB signaling pathway in lung and uterine tissues ( 43 , 44 ). In this study, the phosphorylation of IκB-α and p65 proteins in mammary tissues were increased significantly after S. aureus infection, which indicated that NF-κB was involved in the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways

Liu

Ding

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

Mastitis is an economically important disease in dairy cows, which is often caused by Staphylococcus aureus ( S. aureus ). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by S. aureus . The S. aureus -induced rat model was established and three doses (0.2, 2, 20 μg/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1β, TNF-α, and IL-6, were detected using qRT-PCR. The key proteins of NF-κB and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease S. aureus load in the tissues. The overexpression levels of IL-1β, TNF-α, and IL-6 induced by S. aureus were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-κB and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused by S. aureus and alleviate the inflammatory response.

show abstract

“…Numerous studies have revealed that Clostridium tyrobutyricum could be used to produce higher butyric acid via bacterial fermentation (5)(6)(7). Recently, C. tyrobutyricum has been revealed to protect barrier function in different tissues, including the gut (8), testis (9), and endometrium (10). A new study about the genomic analysis of C. tyrobutyricum pointed out that C. tyrobutyricum is a potential bacteria in regulating health in human beings (11).…”

Section: Introductionmentioning

confidence: 99%

A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum

et al. 2021

View full text Add to dashboard Cite

Probiotics are clinically used for diarrhea and inflammatory bowel diseases in both humans and animals. Previous studies have shown that Clostridium tyrobutyricum (Ct) protects against intestinal dysfunction, while its regulatory function in the gut needs further investigation and the related mechanisms are still not fully elucidated. This study aims to further verify the protective function of Ct and reveal its underlying mechanisms in alleviating diarrhea and intestinal inflammation. Ct inhibited LPS-induced diarrhea and intestinal inflammation in the ileum. IL-22 was identified and the protective role of Ct in the ileum presented an IL-22-dependent manner according to the transcriptomic analysis and in vivo interference mice experiments. The flow cytometric analysis of immune cells in the ileum showed that Ct enhanced the proportions of Th17 cells in response to LPS. The results of in situ hybridization further verified that Ct triggered Th17 cells to produce IL-22, which combined with IL-22RA1 expressed in the epithelial cells. Moreover, Ct was unable to enhance the levels of short-chain fatty acids (SCFAs) in the ileum, suggesting that the protective role of Ct in the ileum was independent of SCFAs. This study uncovered the role of Ct in alleviating diarrhea and inflammation with the mechanism of stimulating Th17 cells in the lamina propria to produce IL-22, highlighting its potential application as a probiotic for diarrhea and inflammation in the ileum.

show abstract

Clostridium tyrobutyricum alleviates Staphylococcus aureus-induced endometritis in mice by inhibiting endometrial barrier disruption and inflammatory response

Abstract: Endometritis is an inflammatory disease of the uterus caused by bacterial infection, and it affects both human and animal health.

Cited by 31 publications

References 45 publications

A Potential Prophylactic Probiotic for Inflammatory Bowel Disease: The Overall Investigation of Clostridium tyrobutyricum ATCC25755 Attenuates LPS‐Induced Inflammation via Regulating Intestinal Immune Cells

A Potential Prophylactic Probiotic for Inflammatory Bowel Disease: The Overall Investigation of Clostridium tyrobutyricum ATCC25755 Attenuates LPS‐Induced Inflammation via Regulating Intestinal Immune Cells

Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways

A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum

Contact Info

Product

Resources

About