Clostridium Perfringenstype A Toxin Production in 3 Commonly Used Culture Media

Abstract

Abstract. In vitro toxin production is an important tool not only for diagnostic purposes but also for the study of pathogenesis of Clostridium perfringens infections. The present study was carried out to compare the level of toxin production by several strains of C. perfringens type A, isolated from the intestine of animals, when cultured in 3 different conventional culture media. Six strains of C. perfringens type A isolated from the small intestine of healthy sheep were cultured in commercial cooked meat medium (CMM), brain heart infusion (BHI), and tryptone glucose yeast (TGY). Intravenous lethality in mice and phospholipase C (PLC) activity were measured in filtered culture supernatants. Lethality of culture supernatants was highest for all isolates when grown in BHI, followed by CMM. No supernatants from any isolates grown in TGY produced lethality in mice. Phospholipase C activity was highest when the isolates were grown in BHI and CMM and significantly lower when grown in TGY.

Dynamics of plc gene transcription and α-toxin production during growth of Clostridium perfringens strains with contrasting α-toxin production

“…The reproducibility of the levels of a-toxin accumulation measured in supernatants of the batch cultures combined with the fact that only insignificant levels of the toxin were detectable in cell lysates, support the use of secreted a-toxin as a measure of the strain-specific production pattern in accordance with previously reported observations (Abildgaard et al, 2009;Gholamiandekhordi et al, 2006). It is however important to emphasize that comparison should only be made between strains grown under comparable conditions, since, e.g., culture media and pH have been reported to influence toxin production levels of C. perfringens significantly (Fernandez-Miyakawa et al, 2007;Mö llby et al, 1976).…”

Sequence variation in the α-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens

“…perfringens type A may vary considerably depending on the culture media used (Fernandez-Miyakawa et al, 2007). But without doubt, the new and important reports on other C. perfringens virulence factors, like NetB (Keyburn et al, 2008) and proteolytic enzyme (collagenase) activity (Olkowski et al, 2008), in relation to clinical and subclinical NE call for a thorough evaluation of the potential virulence factors and our understanding of the disease.…”

The CpAL Quorum Sensing System Regulates Production of Hemolysins CPA and PFO To Build Clostridium perfringens Biofilms

“…We therefore investigated whether the culture medium would impact the formation of C. perfringens biofilms. To assess this, we used two classic C. perfringens broth media, FTG and TGY (59). As shown in Fig.…”