<i>Clostridioides difficile</i>
            aggravates dextran sulfate solution (DSS)-induced colitis by shaping the gut microbiota and promoting neutrophil recruitment

Dong, Danfeng; Su, Tongxuan; Chen, Wei; Wang, Daosheng; Xue, Yuezhen; Lu, Qiuya; Jiang, Cen; Ni, Qi; Mao, Enqiang; Peng, Yibing

doi:10.1080/19490976.2023.2192478

Cited by 9 publications

(3 citation statements)

References 57 publications

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“…The qPCR analyses revealed that, as the disease progressed, Escherichia coli abundance increased while Bacteroides vulgatus abundance decreased (Supplemental Figure S5(e)). The results of our prior study of microbiota changes in DSS mice using 16S rRNA sequencing 23 were consistent with the present findings (Supplemental Figure S5(f)). In summary, our findings thus far revealed cross-species homogeneity of serum BA changes in IBD.…”

Section: Resultssupporting

confidence: 92%

Bile acid profiling as an effective biomarker for staging in pediatric inflammatory bowel disease

Chen,

Wang,

Deng

et al. 2024

Gut Microbes

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Rapid and accurate clinical staging of pediatric patients with inflammatory bowel disease (IBD) is crucial to determine the appropriate therapeutic approach. This study aimed to identify effective, convenient biomarkers for staging IBD in pediatric patients. We recruited cohorts of pediatric patients with varying severities of IBD to compare the features of the intestinal microbiota and metabolites between the active and remitting disease stages. Metabolites with potential for staging were targeted for further assessment in both patients and colitis model mice. The performance of these markers was determined using machine learning and was validated in a separate patient cohort. Pediatric patients with IBD exhibited distinct gut microbiota structures at different stages of disease activity. The enterotypes of patients with remitting and active disease were Bacteroides-dominant and Escherichia-Shigella-dominant , respectively. The bile secretion pathway showed the most significant differences between the two stages. Fecal and serum bile acid (BA) levels were strongly related to disease activity in both children and mice. The ratio of primary BAs to secondary BAs in serum was developed as a novel comprehensive index, showing excellent diagnostic performance in stratifying IBD activity (0.84 area under the receiver operating characteristic curve in the primary cohort; 77% accuracy in the validation cohort). In conclusion, we report profound insights into the interactions between the gut microbiota and metabolites in pediatric IBD. Serum BAs have potential as biomarkers for classifying disease activity, and may facilitate the personalization of treatment for IBD.

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Section: Resultssupporting

confidence: 92%

Bile acid profiling as an effective biomarker for staging in pediatric inflammatory bowel disease

Chen,

Wang,

Deng

et al. 2024

Gut Microbes

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“…The immune system characteristics of C57BL/6 mice are similar to humans, making them a good representative model for studying IBD [ 21 ]. DSS, by inducing damage to the colonic mucosa and eliciting inflammatory responses in mice, effectively simulates the pathophysiological process of UC [ 22 ]. Therefore, it is widely used in the study of IBD, particularly as an experimental animal model for UC.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of synbiotic combination of Lactobacillus plantarum SC-5 and olive oil extract tyrosol in a murine model of ulcerative colitis

Yu,

Hu,

Ren

et al. 2024

J Transl Med

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Background Ulcerative colitisis (UC) classified as a form of inflammatory bowel diseases (IBD) characterized by chronic, nonspecific, and recurrent symptoms with a poor prognosis. Common clinical manifestations of UC include diarrhea, fecal bleeding, and abdominal pain. Even though anti-inflammatory drugs can help alleviate symptoms of IBD, their long-term use is limited due to potential side effects. Therefore, alternative approaches for the treatment and prevention of inflammation in UC are crucial. Methods This study investigated the synergistic mechanism of Lactobacillus plantarum SC-5 (SC-5) and tyrosol (TY) combination (TS) in murine colitis, specifically exploring their regulatory activity on the dextran sulfate sodium (DSS)-induced inflammatory pathways (NF-κB and MAPK) and key molecular targets (tight junction protein). The effectiveness of 1 week of treatment with SC-5, TY, or TS was evaluated in a DSS-induced colitis mice model by assessing colitis morbidity and colonic mucosal injury (n = 9). To validate these findings, fecal microbiota transplantation (FMT) was performed by inoculating DSS-treated mice with the microbiota of TS-administered mice (n = 9). Results The results demonstrated that all three treatments effectively reduced colitis morbidity and protected against DSS-induced UC. The combination treatment, TS, exhibited inhibitory effects on the DSS-induced activation of mitogen-activated protein kinase (MAPK) and negatively regulated NF-κB. Furthermore, TS maintained the integrity of the tight junction (TJ) structure by regulating the expression of zona-occludin-1 (ZO-1), Occludin, and Claudin-3 (p < 0.05). Analysis of the intestinal microbiota revealed significant differences, including a decrease in Proteus and an increase in Lactobacillus, Bifidobacterium, and Akkermansia, which supported the protective effect of TS (p < 0.05). An increase in the number of Aspergillus bacteria can cause inflammation in the intestines and lead to the formation of ulcers. Bifidobacterium and Lactobacillus can regulate the micro-ecological balance of the intestinal tract, replenish normal physiological bacteria and inhibit harmful intestinal bacteria, which can alleviate the symptoms of UC. The relative abundance of Akkermansia has been shown to be negatively associated with IBD. The FMT group exhibited alleviated colitis, excellent anti-inflammatory effects, improved colonic barrier integrity, and enrichment of bacteria such as Akkermansia (p < 0.05). These results further supported the gut microbiota-dependent mechanism of TS in ameliorating colonic inflammation. Conclusion In conclusion, the TS demonstrated a remission of colitis and amelioration of colonic inflammation in a gut microbiota-dependent manner. The findings suggest that TS could be a potential natural medicine for the protection of UC health. The above results suggest that TS can be used as a potential therapeutic agent for the clinical regulation of UC.

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“… 75 And deficiency of suppressors of cytokine signaling 3 in myeloid cells and colonization of Clostridioides difficile result in accumulation of neutrophils. 76 , 77 PTK2B, upregulated in inflamed mucosa of UC patients, could enhance neutrophil migration and reduce the generation of ROS, myeloperoxidase, and antimicrobial peptides (S100A8/A9) in neutrophils. 78 MUC5AC deficiency increases the surface area of the colonic barrier in direct contact with bacteria leading to greater neutrophil frequency and severe DSS-induced colitis.…”

Section: Disease Pathogenesismentioning

confidence: 99%

Research advancements and perspectives of inflammatory bowel disease: A comprehensive review

Bai,

Wang,

et al. 2024

Science Progress

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Inflammatory bowel disease (IBD) is a chronic inflammatory disease with increasing incidence, such as Crohn's disease and ulcerative colitis. The accurate etiology and pathogenesis of IBD remain unclear, and it is generally believed that it is related to genetic susceptibility, gut microbiota, environmental factors, immunological abnormalities, and potentially other factors. Currently, the mainstream therapeutic drugs are amino salicylic acid agents, corticosteroids, immunomodulators, and biological agents, but the remission rates do not surpass 30–60% of patients in a real-life setting. As a consequence, there are many studies focusing on emerging drugs and bioactive ingredients that have higher efficacy and long-term safety for achieving complete deep healing. This article begins with a review of the latest, systematic, and credible summaries of the pathogenesis of IBD. In addition, we provide a summary of the current treatments and drugs for IBD. Finally, we focus on the therapeutic effects of emerging drugs such as microRNAs and lncRNAs, nanoparticles-mediated drugs and natural products on IBD and their mechanisms of action.

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Clostridioides difficile aggravates dextran sulfate solution (DSS)-induced colitis by shaping the gut microbiota and promoting neutrophil recruitment

Cited by 9 publications

References 57 publications

Bile acid profiling as an effective biomarker for staging in pediatric inflammatory bowel disease

Bile acid profiling as an effective biomarker for staging in pediatric inflammatory bowel disease

Protective effect of synbiotic combination of Lactobacillus plantarum SC-5 and olive oil extract tyrosol in a murine model of ulcerative colitis

Research advancements and perspectives of inflammatory bowel disease: A comprehensive review

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