<i>CLE19</i>expressed in the embryo regulates both cotyledon establishment and endosperm development in<i>Arabidopsis</i>

Xu, Tingting; Ren, Shi‐Chao; Song, Xiu‐Fen; Liu, Chunming

doi:10.1093/jxb/erv293

Cited by 37 publications

(27 citation statements)

References 57 publications

(79 reference statements)

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“…The arf5-1, mp-G12 nph4-1 [50], arf2-6 (CS24600) [42], arf3-1 (CS24603) [43], arf4-2 (SALK_070506C) [11], wox1-2 (8AAJ85), and prs (SALK_127850) [23] mutants used in this study are in the Col-0 background. The transgenic lines pARF6::n3GFP, pARF8::n3GFP, pARF19::n3GFP, and pNPH4::n3GFP [51], p35S::DII-Venus [52], pDR5::GFP-ER [53], pDR5v2::ntdTomato pDR5::n3GFP [34], pMP::MP-GFP, pMP::MP-HA [39], pMP::MPΔ [17], pWOX1::SV40-3XGFP, pPRS::SV40-3XGFP [54], and ARF2ox [55] are in Col-0, and pARF3::ARF3-GFP and pARF3::mARF3-GR are in L er [46]. …”

Section: Methodsmentioning

confidence: 99%

Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis

et al. 2017

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The flattening of leaves to form broad blades is an important adaptation that maximizes photosynthesis. However, the molecular mechanism underlying this process remains unclear. The WUSCHEL-RELATED HOMEOBOX (WOX) genes WOX1 and PRS are expressed in the leaf marginal domain to enable leaf flattening, but the nature of WOX expression establishment remains elusive. Here, we report that adaxial-expressed MONOPTEROS (MP) and abaxial-enriched auxin together act as positional cues for patterning the WOX domain. MP directly binds to the WOX1 and PRS promoters and activates their expression. Furthermore, redundant abaxial-enriched ARF repressors suppress WOX1 and PRS expression, also through direct binding. In particular, we show that ARF2 is redundantly required with ARF3 and ARF4 to maintain the abaxial identity. Taken together, these findings explain how adaxial-abaxial polarity patterns the mediolateral axis and subsequent lateral expansion of leaves.

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Section: Methodsmentioning

confidence: 99%

Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis

et al. 2017

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“…Periodic acid-Schiff's reagent (PAS) staining was used to analyze vascular cytohistological structure, which was performed on semi-thin sectioned leaf veins embedded in LR white resin, as previously described [52].…”

Section: Cytohistological Analysesmentioning

confidence: 99%

CsIVP functions in vasculature development and downy mildew resistance in cucumber

et al. 2020

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Domesticated crops with high yield and quality are frequently susceptible to pathogen attack, whereas enhancement of disease resistance generally compromises crop yield. The underlying mechanisms of how plant development and disease resistance are coordinately programed remain elusive. Here, we showed that the basic Helix-Loop-Helix (bHLH) transcription factor Cucumis sativus Irregular Vasculature Patterning (CsIVP) was highly expressed in cucumber vascular tissues. Knockdown of CsIVP caused severe vasculature disorganization and abnormal organ morphogenesis. CsIVP directly binds to vascularrelated regulators YABBY5 (CsYAB5), BREVIPEDICELLUS (CsBP), and AUXIN/INDOLE-ACETIC ACIDS4 (CsAUX4) and promotes their expression. Knockdown of CsYAB5 resulted in similar phenotypes as CsIVP-RNA interference (RNAi) plants, including disturbed vascular configuration and abnormal organ morphology. Meanwhile, CsIVP-RNAi plants were more resistant to downy mildew and accumulated more salicylic acid (SA). CsIVP physically interacts with NIM1-INTERACTING1 (CsNIMIN1), a negative regulator in the SA signaling pathway. Thus, CsIVP is a novel vasculature regulator functioning in CsYAB5-mediated organ morphogenesis and SA-mediated downy mildew resistance in cucumber.

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“…Further substitutions of the Gly residue individually with the other 18 possible proteinaceous amino acids determined that the Gly-to-Thr substitution resulted in the strongest antagonistic effect in the wild type (Song et al, 2012(Song et al, , 2013. With this strategy, the peptide signal CLE19 was revealed to be important for cotyledon establishment in embryos and endosperm development (Xu et al, 2015).…”

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confidence: 99%

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Wang

Song

et al. 2017

Plant Physiol.

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The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN) and overexpression (OX) transgenic plants of the CLE19 gene, exhibited significantly reduced anther size and pollen grain number and abnormal pollen wall formation in Arabidopsis (Arabidopsis thaliana). Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface, but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae. With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated and swollen aborted microspores (ams)-like tapetal cells, lacked lipidic tapetosomes and elaioplasts, and had abnormal pollen primexine without obvious accumulation of sporopollenin precursors. Moreover, CLE19 is important for the normal expression of more than 1,000 genes, including the transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(2/2) plants exhibited the ams anther phenotype and ams(+/2) partially suppressed the DN-CLE19 transgeneinduced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.Pollen grains are generated in the male reproductive organ anther of flowering plants and are essential for plant fertility. In the model plant Arabidopsis (Arabidopsis thaliana), pollen grains are ellipsoidal, and the pollen surface is covered by a reticulate exine. In contrast, rice (Oryza sativa) pollen grains are globular and have a smooth surface without the reticulate structure. A well-organized pollen wall structure is essential for the physical and chemical stability of the mature pollen by providing protection for pollen grains from environmental stresses, such as desiccation and microbial attacks (Scott et al., 2004). The pollen wall is composed mainly of three layers: the intine layer, the exine layer, and the pollen coat (Zinkl et al., 1999;Edlund et al., 2004;Blackmore et al., 2007). The intine is the innermost layer of the pollen wall immediately adjacent to the plasma membrane of the pollen vegetative cell and is composed mainly of pectin, cellulose, hemicellulose, hydrolytic enzymes, and hydrophobic proteins (Scott et al., 2004). Both the exine layer and the pollen coat layer are basically of a lipidic nature. The exine is largely formed from acyl lipid and phenylpropanoid precursors, which together form the mixed stable biopolymer known as sporopollenin (Gui...

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CLE19expressed in the embryo regulates both cotyledon establishment and endosperm development inArabidopsis

Abstract: Highlight CLE19, as an embryo-expressed CLV3/ESR gene, regulates cotyledon establishment in embryos and nuclear proliferation and cellularization in the endosperm.

Cited by 37 publications

References 57 publications

Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis

Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis

CsIVP functions in vasculature development and downy mildew resistance in cucumber

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

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