<i>Citrate Metabolism in Uremia</i>

Pierce, James M.; Guild, Warren R.; Merrill, John P.

doi:10.1152/jappl.1957.11.2.231

Cited by 6 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate whether the self‐assembly with Eu 2 ⋅ 1 could be affected by other endogenous ions than phosphate, we studied the binding of citrate and lactate in methanol, and we were not able to observe any binding. So to investigate the effect of arterial concentrations of lactate (2 m m ), pyruvate (0.3 m m ), and citrate (0.01 m ), Eu 2 ⋅ 1 was titrated with dinicotinate in BBS at pH 8.2 in the presence of arterial concentrations of these. The results show that all three ions exhibit competitive binding.…”

Section: Resultsmentioning

confidence: 99%

Thermodynamics of Self‐Assembly of Dicarboxylate Ions with Binuclear Lanthanide Complexes

2015

View full text Add to dashboard Cite

Self-assembly of a range of carboxylic acids (benzoic acid, dinicotinic acid, nicotinic acid, and isophthalic acid) with the europium complex of 5-nitro-α,α′-bis(DO3Ayl)-m-xylene (where DO3A is 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid) has been explored to establish the thermodynamics of binding in a range of solvent systems and in a range of aqueous buffer solutions. In this system, profound effects are observed as a consequence of competition by the hydroxide ion, which outcompetes even dinicotinate at high pH. In the case of isophthalate, which binds most strongly, and dinicotinate, both enthalpic and entropic contributions to binding have been identified. The europium complex with 5-nitro-α,α′-bis(DO3Ayl)-m-xylene is found to have a solution structure significantly different from the related europium complex of 5-amino-α,α′-bis(DO3Ayl)-m-xylene. It is found that phosphate binds strongly to the europium complex of the nitro derivate but not to the europium complex of amino derivative. Lactate, citrate, and pyruvate also bind strongly to 5-nitro-α,α′-bis(Eu⋅DO3Ayl)-m-xylene, and it is concluded that the solution structure of this binuclear lanthanide complex is significantly different from that of the amino-substituted complex.

show abstract

Section: Resultsmentioning

confidence: 99%