Cissus quadrangularis Linn exerts dose‐dependent biphasic effects: osteogenic and anti‐proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblasts

Abstract

These findings suggest that CQ extract revealed concentration-dependent biphasic effects, which would contribute notably to future assessment of pre-clinical efficacy and safety studies.

Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression

“…Our result was opposite to the recent finding that 100 ppm ethanol extract of CQ significantly induced apoptosis, by activation of ROS (reactive oxygen species) formation and DNA fragmentation in A431, human epidermoid carcinoma cell line [36]. Moreover, the biphasic properties of the ethanolic extract of CQ were dependent on the concentration by which the extract at 75 and 100 ppm impaired osteoblasts viability, proliferation, and minernalisation [37]. However, the in vivo model supported the use of the ethanol extracts, without partition, at a high dose of up to 500 mg/kg, which caused no toxicity to the tested animals [20][21][22][23].…”

Silymarin nanoemulsion against human hepatocellular carcinoma: development and optimization

“…Further, SLN at a concentrations 2.5 and 5 lg/ml reduced the viability of cells to 28.45 and 15.89% (p < .05), respectively ( Figure 6(A,B)). The toxicity of oligonucleotide/cationic complexes reduced significantly by the SLN [34]. Although effects of silymarin alone were given on HepG2 cells before [41,42] but the present study showed better effects as SLN reduces the cell viability of cancer cell line in a dose-dependent manner without harming the surrounding Chang liver cells (normal cells) ( Figure 7).…”

“…Intracellular ROS generation was analyzed by using fluorescence microscopic imaging technique as per previous protocol [34]. Cells (1 Â 10 4 per well) were exposed at three effective concentrations i.e.…”

Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuation

“…The proliferative effect of GS was determined by MTT assay as per protocol [ 22 ]. Briefly, cultured osteoblasts at a density of 2 × 10 3 cells/well were seeded in 96-well plate and incubated with GS solution prepared in culture media at concentrations 1, 5 and 10 μM in triplicate for 48 h. The 17β-estradiol (E2) at 1 nM was considered as a positive control because it can produce maximum induction in osteoblasts as per organisation for economic co-operation and development (OECD) guidelines.…”