Chrysopa septempunctata(Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching

Abstract

Vitellogenin (Vg) is a precursor of major egg storage protein, vitellin (Vt), and plays primary roles in reproduction of oviparous vertebrates and invertebrates. Chrysopa septempunctata Wesmael is an important and common predator of various insect pests. Here, we first cloned C. septempunctata Vg gene, CsVg. The complete CsVg cDNA was 5664 bp, which encodes an 1810-residues protein with a predicted molecular mass of 206.23 kDa. Expression profile revealed that CsVg mRNA first appeared on day 4 after emergence, maximally accumulated on day 10, and then declined gradually. RNAi mediated by injection of dsRNA depleted CsVg transcripts, significantly reduced egg-laying amount, and decreased egg hatching rate, suggesting that CsVg functions through effects on egg production and hatching in C. septempunctata.

Identification of Development-Related Genes in the Ovaries of Adult Harmonia axyridis (Pallas) Lady Beetles Using a Time- Series Analysis by RNA-seq

“…Wesmael32. Based on the sequence alignment with the results in this study, the TRINITY_DN20736_c0_g1 sequence shows 46.52% similarity with the vitellogenin gene reported in the study of Liu (2015)32. This finding suggests that these two genes have a certain similarity.…”

“…Liu (2015) studied the function of the vitellogenin gene in Chrysopa septempunctata. Wesmael32. Based on the sequence alignment with the results in this study, the TRINITY_DN20736_c0_g1 sequence shows 46.52% similarity with the vitellogenin gene reported in the study of Liu (2015)32.…”

Nutritional and reproductive signaling revealed by comparative gene expression analysis in Chrysopa pallens (Rambur) at different nutritional statuses

“…This is not consistent with results implied by phylogenetic trees of ILPs and InRs (Figs 4 and 5). So far, the close evolutionary relationship between Neuroptera and Coleoptera has been confirmed by the prior transcriptome [27] and molecular trees based on 18S rRNA and Vgs [29,30]. As BLASTX results were based on alignment analysis of tens of thousands of sequences, we hypothesize that phylogenetic relationship revealed by species distribution (Fig 1) should be more reliable than that suggested by molecular tree based on a single gene.…”

“…The green lacewing, Chrysopa pallens , is among the most effective entomophagous predators because of wide range of prey, extensive distribution and excellent predatory performance at both larval and adult stages [28, 29]. In 2013, the first transcriptome analysis in C .…”

“…The housekeeping gene C . pallens actin was used as internal control for normalization [29]. Sequence specific primers were designed with Primer Premier 5.0 and shown in S4 Table.…”