<i>Chlamydia trachomatis</i>Genotypes: Correlation with Clinical Manifestations of Infection and Patients' Characteristics

Duynhoven, Y. T. H. P. Van; Ossewaarde, Jacobus M.; Derksen-Nawrocki, R. P.; Meijden, W I van der; Laar, M J W van de

doi:10.1086/516291

Cited by 60 publications

(51 citation statements)

References 40 publications

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“…This is in agreement with other studies performed in different parts of the world (8,15,17,18). With the RLB developed we found that 91.3% of the women showed single C. trachomatis infections and 8.7% showed multiple infections.…”

Section: Discussionsupporting

confidence: 93%

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

et al. 2004

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In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A nested VD2 PCRreverse line blot (RLB) assay was developed for the typing of nine different urogenital serovars of C. trachomatis. The assay developed was tested with reference strains of C. trachomatis serovars and cervical scrapes of 86 Colombian women previously found to be positive for C. trachomatis by using plasmid PCR. Two sets of primers directed to the VD2 region of the omp1 gene of C. trachomatis were designed, and fragments of 220 and 166 bp were generated in the primary and nested PCRs, respectively. In addition, an RLB assay was developed to identify nine different urogenital serovars of C. trachomatis (Ba, D, E, F, G, H, I, J, and K) and group controls, including group B (Ba, D, and E), group C (I, J, K, and H), and an intermediate group (F and G). Using this assay, we were able to type 81 of the 86 samples (94.2%). Of these samples, 91.3% were single C. trachomatis infections, and 8.7% were multiple infections. The most common serovars identified were serovars D (22.2%), F (18.5%), G (13.6%), and E (12.3%). Of the women with multiple C. trachomatis infections, >50% contained both serovars D and E. The nested VD2 PCR-RLB developed is a simple, fast, and specific method for the identification of individual urogenital C. trachomatis serovars previously detected by using plasmid PCR. Moreover, it is an appropriate method for studying multiple C. trachomatis infections and for use in large epidemiological studies.Chlamydia trachomatis is one of the most common pathogens of sexually transmitted diseases worldwide.Initially, 15 prototypic serovars labeled A to K and L1, L2, and L3 were identified based on immunoepitope analysis of the major outer membrane protein (MOMP) with monoclonal and polyclonal antibodies (7, 10). On the basis of the pathogenic potential, the serovars A, B, Ba, and C are commonly associated with trachoma, D to K are commonly associated with urogenital infections, and L1, L2, and L3 are commonly associated with lymphogranuloma venereum. In addition, based on amino acid similarity, the serovars have been grouped in three different serogroups: group B (B, Ba, D, Da, E, L1, L2, and L2a), group C (A, C, H, I, Ia, J, Ja, K and L3), and an intermediate group (F and G) (20).The typing of C. trachomatis infection is important for epidemiological and vaccination studies. In addition, the knowledge of pathogenesis and transmission rates of C. trachomatis serovars are important topics in clinical and basic research (1,3,14).To study the epidemiology of C. trachomatis infections, different laboratory techniques for C. trachomatis serovar identification have been developed in the last few years. These techniques include standard MOMP serotyping, restriction fragment length polymorphism (RFLP) analysis of the PCRamplified omp1 gene, and nucleotide sequencing of the omp1 gene (3,4,12,13,19). Compared to imm...

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Section: Discussionsupporting

confidence: 93%

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

et al. 2004

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“…RFLP analysis found a heterogeneous population of C. trachomatis in this student population. These results are in agreement with those reported in other areas (Brunham et al, 1996;Sturm-Ramirez et al, 2000;van de Laar et al, 1996;van Duynhoven et al, 1998). The predominance of class B genotypes (57 %) in this study and in many others may suggest a real biological advantage over other genotypes (Sturm-Ramirez et al, 2000).…”

Section: Resultssupporting

confidence: 93%

“…Genotypes D/Da, F and G were also common in both sexes. The observed distribution of genotypes has also been described in many studies (Batteiger et al, 1989;Morré et al, 2000;van de Laar et al, 1996;van Duynhoven et al, 1998).…”

Section: Resultssupporting

confidence: 73%

Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students

Ngandjio

Clerc

Fonkoua

et al. 2004

Journal of Medical Microbiology

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Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR-and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n ¼ 14; 40 %), followed by F (n ¼ 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories. IntroductionChlamydia trachomatis is the causative agent of a variety of diseases and syndromes, including trachoma, urogenital infections and lymphogranuloma venereum, depending on the biovar and serovar of the strains involved. Currently, 19 human serovars and numerous variants have been identified with the use of polyclonal and monoclonal antibodies against the major outer-membrane protein (MOMP). Serovars A-C are most often associated with trachoma and serovars L1-L3 are associated with lymphogranuloma venereum, while serovars D-K are primarily associated with urogenital and neonatarum infections (Morré et al., 1998a;Ossewaarde et al., 1994;Sturm-Ramirez et al., 2000). Various studies have shown the feasibility of typing clinical isolates by PCR-based RFLP analysis of the amplified omp1 gene encoding MOMP (Frost et al., 1991;Rodriguez et al., 1991;Sayada et al., 1991), avoiding the costly production of monoclonal antibodies. This method was originally performed on cell cultures of cervical and urethral specimens. Nowadays, the direct typing of cervical specimens by a nested omp1 PCR-RFLP assay is used for the differentiation of isolates into genotypes (Lan et al., 1993(Lan et al., , 1994. Nevertheless, typing of C. trachomatis is not routine in many African laboratories. Up to now, only one study showing the genotypic diversity of genital C. trachomatis in sub-Saharan Africa has been performed (SturmRamirez et al., 2000). Although RFLP analysis of an unknown strain consists of comparing the patterns with those of reference strains, patterns of all reference strains have not yet been published. Therefore, the aim of this study was to present RFLP patterns of 18/19 omp1-amplified reference strains, in order to promote typing of C. trachomatis by RFLP analysis in many laboratories with limited resources. In addition, this study was intended to describe the distribution of C. trachomatis genotypes in urogenital specimens, collected from Cameroonian students residing in Yaounde. omp1 amplification. Eighteen reference and 2...

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“…nvCT comprised 64% of chlamydia-positive specimens over the study period in Dalarna as a whole, and up to 78% in some localities (7); elsewhere in Sweden (8-10) and worldwide (11,12), genotype E strains of different subvariants comprise ≈40% of chlamydia-positive speciments in heterosexual populations. These data might indicate that the high proportion of nvCT is not only a result of accumulation of chlamydia cases when diagnostics failed and treatment and contact tracing were inadequate.…”

Section: Discussionmentioning

confidence: 99%

Emergence and Spread ofChlamydia trachomatisVariant, Sweden

Herrmann¹,

Törner²,

Low³

et al. 2008

Emerg. Infect. Dis.

102

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Chlamydia trachomatisGenotypes: Correlation with Clinical Manifestations of Infection and Patients' Characteristics

Cited by 60 publications

References 40 publications

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students

Emergence and Spread ofChlamydia trachomatisVariant, Sweden

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