<i>Chino del tomate virus</i>:Relationships to Other Begomoviruses and Identification of A-Component Variants that Affect Symptom Expression

Brown, Judith K.; Ostrow, Kristin M.; Idris, A. M.; Stenger, Drake C.

doi:10.1094/phyto.2000.90.5.546

Cited by 24 publications

(20 citation statements)

References 36 publications

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“…Interestingly, infectivity (efficiency) and virulence (symptom severity) varied with the particular reassortant-host combination. The REPbinding sites are identical among this suite of cucurbit-infecting begomoviruses (15,35) and in the bean-adapted relative BCaMV, which does not infect cucurbits (13). The finding that MCLCuV-GT encodes the same REP-binding site as the former three species but shares in common only the hosts of CuLCrV (watermelon), SLCV (watermelon), SMLCV (pumpkin), and BCaMV (bean), with their respective viruses, was unexpected, given the overly simplistic hypothesis examined herein, in which viruses sharing iterons and host range were expected to reassort.…”

Section: Discussionmentioning

confidence: 93%

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Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at ϳ90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

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Section: Discussionmentioning

confidence: 93%

“…It comprises five closely related species that show little or no evidence of recombination (12,13). Wild-type parent virus(es) and certain reassortants exhibited differential capacities for host shifting and degrees of virulence, indicating that they are readily responsive to genetic factors associated with virus-host interactions (3).…”

Section: Discussionmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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“…The rapid and widespread appearance of CuLCV in cucurbit cultivation represents the most recent example of begomovirus emergence in the American Southwest and northern Mexico. Previous examples of begomovirus emergence in the region include SLCV-E and SLCV-R (11,16,27) in cucurbits, BCaMV in bean (8), PepGMV and Pepper huasteco yellow vein virus in peppers (39,43), and Chino del tomate virus (9) and Tomato leaf curl Sinaloa virus (22) in tomato. Emergent begomoviruses become noticed primarily due to the new economic consequences of the diseases they cause in cultivated crops and to altered host range or virulence.…”

Section: Discussionmentioning

confidence: 99%

Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Brown¹,

Idris²,

Alteri³

et al. 2002

Phytopathology®

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Cucurbit leaf curl virus (CuLCV), a whitefly-transmitted geminivirus previously partially characterized from the southwestern United States and northern Mexico, was identified as a distinct bipartite begomovirus species. This virus has near sequence identity with the previously partially characterized Cucurbit leaf crumple virus from California. Experimental and natural host range studies indicated that CuLCV has a relatively broad host range within the family Cucurbitaceae and also infects bean and tobacco. The genome of an Arizona isolate, designated CuLCV-AZ, was cloned and completely sequenced. Cloned CuLCV-AZ DNA A and B components were infectious by biolistic inoculation to pumpkin and progeny virus was transmissible by the whitefly vector, Bemisia tabaci, thereby completing Koch's postulates. CuLCV-AZ DNA A shared highest nucleotide sequence identity with Squash leaf curl virus-R (SLCV-R), SLCV-E, and Bean calico mosaic virus (BCaMV) at 84, 83, and 80%, respectively. The CuLCV DNA B component shared highest nucleotide sequence identity with BCaMV, SLCV-R, and SLCV-E at 71, 70, and 68%, respectively. The cis-acting begomovirus replication specificity element, GGTGTCCTGGTG, in the CuLCV-AZ origin of replication is identical to that of SLCV-R, SLCV-E, and BCaMV, suggesting that reassortants among components of CuLCV-AZ and these begomoviruses may be possible. Reassortment experiments in pumpkin demonstrated that both reassortants of CuLCV-AZ and SLCV-E A and B components were viable. However, for CuLCV-AZ and SLCV-R, only one reassortant (SLCV-R DNA A/CuLCV-AZ DNA B) was viable on pumpkin, even though the cognate component pairs of both viruses infect pumpkin. These results demonstrate that reassortment among sympatric begomovirus species infecting cucurbits are possible, and that, if generated in nature, could result in begomoviruses bearing distinct biological properties.

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“…Full-length A and B DNA components of PepGMV-Mo and PepGMV-Ser were cloned into pGEM7zF+ using a procedure previously used to clone other bipartite begomovirus genomes (7,8). Total DNA was extracted from pepper infected with each begomovirus isolate, digested with various restriction endonucleases, and analyzed by Southern hybridization using riboprobes of virion sense transcribed from pTPV-DA or pTPV-DB (data not shown).…”

Section: Methodsmentioning

confidence: 99%

“…However, relationships among these begomovirus isolates remained unclear due to a lack of nucleotide sequence data and a variety of biological differences, including mechanical transmissibility, host range, and symptom phenotype. The situation was further confounded by contemporary isolation of several begomoviruses commonly found in tomato that also infect pepper (5,6,8,23).…”

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confidence: 99%

Genetic and Phenotypic Variation of the Pepper golden mosaic virus Complex

Brown¹,

Idris²,

Ostrow³

et al. 2005

Phytopathology®

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Chino del tomate virus:Relationships to Other Begomoviruses and Identification of A-Component Variants that Affect Symptom Expression

Cited by 24 publications

References 36 publications

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Genetic and Phenotypic Variation of the Pepper golden mosaic virus Complex

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