CCDC3 is specifically upregulated in omental adipose tissue in subjects with abdominal obesity

Abstract

Objective: The aim of this study was to search for novel markers of visceral adiposity. Methods: Visceral (omental) and subcutaneous adipose tissues were obtained from 43 Japanese men. Microarray analysis using total RNA from visceral and subcutaneous adipose tissues obtained from five men with abdominal obesity and five nonobese men was first conducted. Then the expression pattern of candidate genes identified in the human study in mouse models of adiposity was examined. Results: Among 30,500 genes evaluated, the mRNA expression of CCDC3 (encoding coiled-coil domaincontaining protein 3) was upregulated in omental adipose tissues from abdominally obese subjects (3.07-fold) but not in subcutaneous adipose tissues (0.89-fold). Similar expression patterns were found in two distinct mouse models of obesity. In the analysis of all 43 men, CCDC3 mRNA levels in omental, but not in subcutaneous adipose tissue, were positively correlated with waist circumference and body mass index. CCDC3 was predicted to be a secretory protein, which was confirmed by western blotting, as overexpressed CCDC3 was secreted into the culture media. Conclusions: The expression of CCDC3 is specifically increased in visceral adipose tissues in abdominally obese subjects. These results suggest that CCDC3 is a potential biomarker for estimating visceral adiposity.

Synergistic Effects of Hyperandrogenemia and Obesogenic Western-style Diet on Transcription and DNA Methylation in Visceral Adipose Tissue of Nonhuman Primates

“…Importantly, the present study showed that these genes are also differentially expressed in rhesus WAT. CCDC 3 has been previously shown to be upregulated in omental WAT of obese subjects and exerts adipogenic effects in vitro and in vivo 76,77 . In the present study, CCDC3 was found to be upregulated and differentially methylated at the same intronic region by either WSD or T + WSD treatment, suggesting a diet-specific mechanism of gene regulation.…”

A genomewide association mapping study using ultrasound‐scanned information identifies potential genomic regions and candidate genes affecting carcass traits in Nellore cattle

“…The CLSTN2 (calsyntenin 2) is associated with obesity in mammals, particularly in the process of increasing the adipocytes in visceral tissues and subcutaneous fat (Ugi et al . ), and its expression was related to metabolic disorders such as glucose metabolism disorder, disorder of the pancreas, diabetes mellitus, non‐insulin‐dependent diabetes mellitus and insulin‐dependent diabetes mellitus (Mas et al . ).…”

“…In turn, two SNPs associated with REA in Chr 3 were in the DPYD gene (dihydropyrimidine dehydrogenase) that encodes an enzyme linked to the first step of the thymine and uracil catabolism (Takai et al 1994). The CLSTN2 (calsyntenin 2) is associated with obesity in mammals, particularly in the process of increasing the adipocytes in visceral tissues and subcutaneous fat (Ugi et al 2014), and its expression was related to metabolic disorders such as glucose metabolism disorder, disorder of the pancreas, diabetes mellitus, non-insulin-dependent diabetes mellitus and insulin-dependent diabetes mellitus (Mas et al 2013). Apparently, the FBLN1 and LOC531152 have no direct link with the REA.…”

Survey of SNPs Associated with Total Number Born and Total Number Born Alive in Pig

“…CLSTN2 (calsintenin 2) is associated with obesity in mammals, especially in the process of increasing adipocytes in visceral tissues and in subcutaneous fat [56]. Santana et al [57] defined CLSTN2 as a candidate gene associated with ultrasound-derived measurements of the rib-eye area, backfat thickness and rumpfat thickness in Nellore cattle.…”