<i>Candidatus</i>
            Chloracidobacterium thermophilum: An Aerobic Phototrophic Acidobacterium

Bryant, Donald A.; Costas, Amaya M. Garcia; Maresca, Julia A.; Chew, Aline Gómez Maqueo; Klatt, Christian G.; Bateson, Mary M.; Tallon, Luke J.; Hostetler, Jessica B.; Nelson, Karen E.; Heidelberg, John F.; Ward, David M.

doi:10.1126/science.1143236

Cited by 357 publications

(344 citation statements)

References 19 publications

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“…The main absorption maxima detected in the methanol extracts of pink-pigmented isolates were at 445 and 509 nm, suggesting the presence of a-and bcarotene. Thus, despite the fact that chlorophyllbased phototrophy has recently been demonstrated for some thermophilic representatives of the Acidobacteria (Bryant et al, 2007), our strains did not possess this capability.…”

Section: Colony and Cell Morphologies Of Acidobacterial Isolatescontrasting

confidence: 54%

“…Members of this phylum have been detected by 16S rRNA gene-based molecular surveys in a wide variety of environments, including soils and sediments (Barns et al, 1999;Janssen, 2006), hot springs (Hugenholtz et al, 1998b;Barns et al, 1999;Bryant et al, 2007), peat bogs (Rheims et al, 1996;Dedysh et al, 2006), acidic mining lakes (Kleinsteuber et al, 2008), water distribution systems (Martiny et al, 2005) and caves (Zimmermann et al, 2005;Meisinger et al, 2007). The range of phylogenetic diversity in the Acidobacteria is nearly as great as in the Proteobacteria (Hugenholtz et al, 1998a).…”

Section: Introductionmentioning

confidence: 99%

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Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

et al. 2008

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Fluorescence in situ hybridization (FISH) was applied to estimate the population size of the poorly characterized phylum Acidobacteria in acidic peat sampled from nine different Sphagnumdominated wetlands of Northern Russia. The cell numbers of these bacteria in oxic peat layers ranged from 0.4 Â 10 6 to 1.3 Â 10 7 cells per g of wet peat, comprising up to 4% of total bacterial cells. Substrate-induced growth of acidobacteria was observed after amendment of peat samples with glucose, pectin, xylan, starch, ethanol and methanol, while weak or no response was obtained for acetate, pyruvate, mannitol and cellobiose. Using low-nutrient media and FISH-mediated monitoring of the isolation procedure, we succeeded in obtaining nine strains of acidobacteria in pure cultures. These strains belonged to subdivisions 1 and 3 of the Acidobacteria and represented strictly aerobic, heterotrophic organisms. Except for methanol, the substrate utilization patterns of these isolates matched the results obtained in our substrate-amendment experiments with native peat. All strains were also capable of utilizing galacturonic acid, a characteristic component of the cell wall in Sphagnum spp, which is released during moss decomposition. Most isolates from subdivision 1 were truly acidophilic organisms with the growth optimum at pH 3.5-4.5, while the isolates from subdivision 3 grew optimally at pH 5.5-6.5. Another important phenotypic trait of novel strains was their capability of active growth at low temperatures. Both acidophily and low-temperature growth are consistent with the occurrence of acidobacteria in cold and acidic northern wetlands. The ISME Journal (

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Section: Colony and Cell Morphologies Of Acidobacterial Isolatescontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

et al. 2008

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“…and Ca. C. thermophilum isolates (Pierson and Castenholz, 1974;Bryant et al, 2007). These observations suggest that these three populations harvest primarily 700-to 750-nm light, and finer niche differentiation within this group is precedented by the BChl-d-containing Chlorobiales.…”

Section: Description Of Functional Guildsmentioning

confidence: 81%

“…High-performance liquid chromatographic analysis of pigments extracted from the Mushroom Spring mat will be published elsewhere (M Pagel and DA Bryant, unpublished data), but preliminary results indicated the presence of BChl-d, which replaces BChl-c as the aggregated pigments within the chlorosomes of 'green' chlorophototrophic Chlorobiales. Other Cluster-5 scaffolds contained homologs of the reaction center subunit gene pscA ('OS GSB PscA'; Bryant et al, 2007), pscB, pscD as well as csmC, a gene encoding a chlorosome envelope protein that has no homologs in other chlorosome-containing chlorophototrophs and thus is currently diagnostic for Chlorobiales (Bryant et al, 2011).…”

Section: Major Populations and Their Functional Potentialmentioning

confidence: 99%

Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potential

et al. 2011

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Phototrophic microbial mat communities from 60 1C and 65 1C regions in the effluent channels of Mushroom and Octopus Springs (Yellowstone National Park, WY, USA) were investigated by shotgun metagenomic sequencing. Analyses of assembled metagenomic sequences resolved six dominant chlorophototrophic populations and permitted the discovery and characterization of undescribed but predominant community members and their physiological potential. Linkage of phylogenetic marker genes and functional genes showed novel chlorophototrophic bacteria belonging to uncharacterized lineages within the order Chlorobiales and within the Kingdom Chloroflexi. The latter is the first chlorophototrophic member of Kingdom Chloroflexi that lies outside the monophyletic group of chlorophototrophs of the Order Chloroflexales. Direct comparison of unassembled metagenomic sequences to genomes of representative isolates showed extensive genetic diversity, genomic rearrangements and novel physiological potential in native populations as compared with genomic references. Synechococcus spp. metagenomic sequences showed a high degree of synteny with the reference genomes of Synechococcus spp. strains A and B 0 , but synteny declined with decreasing sequence relatedness to these references. There was evidence of horizontal gene transfer among native populations, but the frequency of these events was inversely proportional to phylogenetic relatedness.

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“…Unraveling the microbial composition and processes occurring in these systems will not only help optimize the technical processes but can also lead to the discovery of novel pathways and organisms. In the era of next-generation sequencing techniques, metagenomics-based approaches have enabled the discovery of a suite of novel microbial pathways (Schleper et al, 2005;Bryant et al, 2007;Ettwig et al, 2010;Carrión et al, 2015) and the expansion of the tree of life (Brown et al, 2015). Some of these discoveries have been made in technical systems; for example, novel candidate phyla and expanded metabolic versatility have been identified in aerobic and anaerobic bioreactors (Wexler et al, 2005;Rinke et al, 2013;Nobu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp.

et al. 2016

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Rapid gravity sand filtration is a drinking water production technology widely used around the world. Microbially catalyzed processes dominate the oxidative transformation of ammonia, reduced manganese and iron, methane and hydrogen sulfide, which may all be present at millimolar concentrations when groundwater is the source water. In this study, six metagenomes from various locations within a groundwater-fed rapid sand filter (RSF) were analyzed. The community gene catalog contained most genes of the nitrogen cycle, with particular abundance in genes of the nitrification pathway. Genes involved in different carbon fixation pathways were also abundant, with the reverse tricarboxylic acid cycle pathway most abundant, consistent with an observed Nitrospira dominance. From the metagenomic data set, 14 near-complete genomes were reconstructed and functionally characterized. On the basis of their genetic content, a metabolic and geochemical model was proposed. The organisms represented by draft genomes had the capability to oxidize ammonium, nitrite, hydrogen sulfide, methane, potentially iron and manganese as well as to assimilate organic compounds. A composite Nitrospira genome was recovered, and amo-containing Nitrospira genome contigs were identified. This finding, together with the high Nitrospira abundance, and the abundance of atypical amo and hao genes, suggests the potential for complete ammonium oxidation by Nitrospira, and a major role of Nitrospira in the investigated RSFs and potentially other nitrifying environments.

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Candidatus Chloracidobacterium thermophilum: An Aerobic Phototrophic Acidobacterium

Cited by 357 publications

References 19 publications

Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat

Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potential

Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp.

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