<i>Candida Albicans</i>
            Vaginitis in a Murine Model is Reduced by Polypeptide-Enriched
            <i>Gastrodia Elata</i>
            Extracts

Cai, X. Y.; Kong, Fange; Wang, Ruochen; Zhai, Siyu; Guan, Xi‐Wen; Zhang, Guirong; Wang, Di

doi:10.2217/fmb-2018-0299

Cited by 10 publications

(4 citation statements)

References 26 publications

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“…The antimicrobial activity of GeP against fungi and gram-positive bacteria has been reported previously (19,20). our previous study demonstrated the antimicrobial activities of GeP using the agar diffusion method (20) and are now investigating the therapeutic efficacy of GEP against vulvovaginal candidiasis in mice (30). in the present study, it was demonstrated that the effects of GeP may derive from the damage caused to cell walls and membranes, triggering the leakage of genetic material and disrupting cellular metabolism.…”

Section: Discussionsupporting

confidence: 51%

Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts

et al. 2019

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The present study aimed to evaluate the antimicrobial activity and the possible mechanisms of activity of polypeptide-enriched Gastrodia elata extracts (GeP) against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, the gram-positive bacterium Staphylococcus aureus and the fungus Candida albicans. The antimicrobial activity of GeP was first confirmed by determining the minimum inhibitory concentration by growth curve analysis. GeP was found to damage the cell wall and membrane of the microorganisms tested, as revealed by the morphological changes visible through scanning electron microscopy, and by the observed leakage of alkaline phosphatase and β-galactosidase from cells. GeP was demonstrated to perturb the metabolism of the microorganisms, especially the tricarboxylic acid cycle, as indicated by the reduced intracellular activity of succinate dehydrogenase, malate dehydrogenase and aTPases, including the na + /K +-aTPase and the ca2 +-aTPase. in addition, GeP caused the leakage of the genetic material of the bacteria and the fungus, as indicated by the increased od 260. The results of the present study indicated that GeP may exert its antimicrobial activity by damaging cell walls and membranes, causing the leakage of genetic material, and by perturbing cellular metabolism.

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Section: Discussionsupporting

confidence: 51%

Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts

et al. 2019

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“…As VAGINNE is derived from the fermentation broth of L. crispatus, L. gasseri, and L. jensenii, we hypothesize that the observed anti-Candida activity is, in part, attributed to the presence of bacteriocin, hydrogen peroxide, and lactic acid [22]. Due to the failure to restore healthy ora, especially Lactobacillus, regular agents used for VVC may recur or promote recurrence [23]. However, in the study, treated with VAGINNE in mice could restore Lactobacillus spp., decreasing the RVVC.…”

Section: Discussionmentioning

confidence: 99%

Microbial Cell Factories The fermentation broth of Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus jensenii vaginal infection of Candida albicans in mice

Horng,

Kuo,

Chen

et al. 2024

Preprint

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Background Vulvovaginal candidiasis (VVC) impacts millions of women globally, adversely affecting their quality of life. The primary cause of VVC is Candida albicans. Imidazole antifungals have been widely utilized in clinical settings for the treatment of VVC. However, these medications are associated with various side effects, including gastrointestinal discomfort, rash, and liver toxicity. Moreover, the emerging challenge of C. albicans developing resistance to azole drugs necessitates the development of new therapies for VVC. Results The main objective of this study was to assess the effect of the VAGINNE, the fermentation broth derived from Lactobacillus crispatus, L. gasseri, and L. jensenii, on VVC. The VVC model induced by C. albicans infection was successfully established in BALB/c mice. Following VAGINNE treatment, the colony-forming unit (CFU) of vaginal lavage was quantified through plating. The extent of the infection and inflammatory response was evaluated using hematoxylin-eosin (H&E) staining and enzyme-linked immunosorbent assay (ELISA), respectively. The fermentation broth of L. crispatus, L. gasseri, and L. jensenii exhibited inhibitory effects on the proliferation of C. albicans and the associated inflammatory response. Additionally, it demonstrated a potentially beneficial effects on the growth of Lactobacillus species. Conclusion These findings highlight the potential utility of the fermentation broth derived from these Lactobacillus strains as an antifungal agent for the treatment of VVC.

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“…The experimental animals were kept in Laboratory Animal Center, Xiamen University (SYXK (Min) 2018-0010). After 3 days of adaptive feeding, as previously described 4 , to induce a pseudoestrus condition, all the mice received the subcutaneous injection with 0.1 ml of estradiol valerate (0.2 mg/ml) (Sichuan Jinke Pharmaceutical Co., Ltd, Sichuan, China) every 2 days throughout the experimental period. After pseudoestrus for 6 days, 10 μl suspension of 5.0×10 6 CFU/ml of C. albicans was inoculated intravaginally continuously for 5 days ( n =24).…”

Section: Methodsmentioning

confidence: 99%

Mechanism of Yixishu lotion in the treatment of vaginitis based on network pharmacology combined with experimental validation: an experimental research study

Huo,

Jing,

Wang

et al. 2023

Annals of Medicine &Amp; Surgery

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Objective: Yixishu lotion (YXSL) originates from the summary of traditional Chinese medicine clinical experience and constantly improves in practice in clinical validation of the exact efficacy of traditional Chinese medicine prescription. To explore the mechanism of YXSL in treating vaginitis and the potential mechanisms based on network pharmacology and experimental verification. Methods: The active components and drug-related targets of YXSL were retrieved from the TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) database, and the target was predicted by the UniProt database. Searching for genes related to ‘vaginitis’ disease in the GeneCards database, a total of 2581 drug targets were obtained. The interaction between proteins (PPI – protein–protein interaction) relationship was obtained by STRING database and visualized by Cytoscape software. Finally, the ‘Bioconductor’ installation package in R software was used to analyze the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of the target. Results: In this study, by the method of network pharmacology, the key active components of YXSL were flavonoids such as quercetin, apigenin, kaempferol, luteolin, β-sitosterol; the main core proteins included MAPK14, TP53, FGF2, ESR1, MAPK3, MAPK1, VEGFA, JUN, IL-6, and the KEGG pathway was mainly involved in MAPK pathway, Th17 pathway, Malaria, TNF pathway, and other signaling pathways. Animal experiments showed that the clinical symptoms and vaginal tissue lesions of the YXSL group and the fluconazole group were improved, and the levels of TNF-α (tumor necrosis factor alpha), IL-6 (interleukin-6), MDA (malondialdehyde), SOD (superoxide dismutase), IL-4, and IFN-γ (interferon-γ) in vaginal tissue and serum were better than the model group. Conclusion: YXSL may achieve its therapeutic effect on vaginitis by reducing the inflammatory response, improving oxidative stress response, and improving body immunity, and it provides a theoretical basis for further research on its pharmacodynamic material basis and mechanism of action.

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Candida Albicans Vaginitis in a Murine Model is Reduced by Polypeptide-Enriched Gastrodia Elata Extracts

Cited by 10 publications

References 26 publications

Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts

Possible mechanisms of the antimicrobial effects of polypeptide‑enriched Gastrodia elata Blume extracts

Microbial Cell Factories The fermentation broth of Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus jensenii vaginal infection of Candida albicans in mice

Mechanism of Yixishu lotion in the treatment of vaginitis based on network pharmacology combined with experimental validation: an experimental research study

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