A 62-year-old man with amphotericin B-resistant Candida krusei spondylodiscitis, following an episode of candidemia caused by the same strain, was successfully treated with caspofungin plus voriconazole. Amphotericin B fungicidal concentrations were better predictors of the clinical outcome than were MICs. This is the first case of C. krusei spondylodiscitis reported in the literature.
CASE REPORTA 62-year-old man was diagnosed with acute myeloid leukemia (FAB-M2) in May 2002. The treatment consisted of idarubicin plus citarabine (3 ϩ 7 regimen), achieving complete remission. In July 2002, consolidation was given without problems. Three months later, intensification chemotherapy (mitoxantrone plus citarabine) was administered. Oral fluconazole (100 mg daily) was given for antifungal prophylaxis. On day 10 after chemotherapy, during the severe neutropenia period, the patient presented with fever, myalgia, and disseminated painful skin nodules. Several blood cultures were positive for C. krusei, but culture of a specimen obtained by fine-needle aspiration of the skin lesion was negative. At that moment, the cumulative dose of fluconazole was 1,500 mg. Antifungal therapy was started with liposomal amphotericin B (3 mg/kg/day) for 2 weeks in association with standard-dose caspofungin for 4 weeks. Other therapeutic measures included removal of the central venous catheter (the tip culture was negative) and administration of filgrastim until neutropenia recovery. In addition, he received oral itraconazole (200 mg twice a day for 4 weeks) as an outpatient. In January 2003, 4 months after the candidemia episode, he presented with fever and severe dorsal back pain. Physical examination did not reveal any neurological deficit. A computed tomography (CT) scan showed a left paravertebral mass, with soft tissue involvement at the D5-D6 level. The magnetic resonance imaging (MRI) findings were consistent with spondylodiscitis ( Fig. 1), and the culture of a specimen collected by CT-guided fine-needle biopsy yielded C. krusei. Because of the presence of high (Ն 8 mg/liter) amphotericin B minimum fungicidal concentrations (MFCs), a combination of standard doses of caspofungin and voriconazole was administered for 6 weeks, with a favorable clinical response. Posterior voriconazole maintenance therapy was given, and surgical treatment was not required. After a 6-month follow-up, the evolution remained good on the basis of clinical and MRI evidence, but the patient died 2 months later due to a relapse of leukemia.The two C. krusei strains isolated from blood (CK-18) and biopsy (CK-19) specimens were identified by molecular techniques, according to the method of Esteve-Zarzoso et al. (9). Strain characterization was performed by PCR amplification and subsequent restriction analysis of the 5.8S-ITS region. To confirm the correct identification, an amplification of partial 26S rRNA gene sequences was carried out. The results obtained were compared with the database available online at the IATA website (http://motor.edinfo.es/iata/), a...