<i>Candida albicans</i> Adherence to Denture Base Material: Chemical Disinfection and the Effect of Acquired Salivary Pellicle Formation

Acosta, Emílio José Tabaré Rodríguez; Silva, Paulo Maurício Batista da; Jacobina, Matheus; Lara, Vanessa Soares; Neppelenbroek, Karin Hermana; Porto, Vinícius Carvalho

doi:10.1111/jopr.12197

Cited by 5 publications

(5 citation statements)

References 47 publications

(57 reference statements)

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“…A short-term chemical disinfection protocol helps treat denture stomatitis. Among the several chemical denture cleansers available on the market, NaOCl and CHG chemical solutions are widely used [ 41 ], although the concentration and exposure time for effective disinfection are still conflicting [ 39 ]. Sodium hypochlorite exhibits a wide range of activity with a shorter duration and is inexpensive.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins

et al. 2023

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This study assessed the efficacy of five denture cleansers on the microbial adherence and surface topography of conventional and CAD/CAM denture base resins. Acrylic resin discs were fabricated using conventional, milling, and 3D printing methods (N = 180). The discs were contaminated with dual species of Candida albicans and Streptococcus mutans biofilm for 72 h and then disinfected with either of the denture cleansers (Fittydent cleansing tablets, 2% Chlorhexidine gluconate, 0.2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite (n = 10). Distilled water served as the control group. The colony-forming units of the microorganisms were calculated, followed by post-treatment surface roughness. Data were statistically analyzed using one-way ANOVA, paired t-test, and post hoc Tukey HSD test (α = 0.05). Among the denture cleansers, 2% Chlorhexidine gluconate, 0.5% sodium hypochlorite, and 1% sodium hypochlorite had the best cleansing effect on the resin discs and demonstrated zero growth of colonies for both the species. Comparing the material groups, the 3D-processed discs showed higher colony-forming units followed by the conventional and CAD/CAM milled group. The highest surface roughness was demonstrated by the 3D-printed discs (0.690 ± 0.08 μm), followed by the conventional (0.493 ± 0.11 μm) and the milled groups (0.301 ± 0.08 μm). The tested chemical denture cleansers affected the Candida albicans and Streptococcus mutans adhesion compared to control discs immersed in distilled water. The clinician may recommend to their patient to use 2% chlorhexidine gluconate for the disinfection of CAD/CAM PMMA denture base materials.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins

et al. 2023

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Section: Discussionmentioning

confidence: 99%

“…NaClO has been accepted as a denture cleaning and disinfecting agent at various concentrations ( Felton et al, 2011 ; Fernandes, Orsi & Villabona, 2013 ; Rodríguez Acosta et al, 2015 ; Salloum, 2014 ). It is also characterized by a broad antibacterial spectrum ( Badaró et al, 2017 ; Rodríguez Acosta et al, 2015 ; Salles et al, 2015 ). However, different concentrations and immersion periods may reduce the physical and mechanical properties of the materials and cause allergic reactions in susceptible individuals ( Chia Shi Zhe et al, 2016 ; Pisani et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of cleaning sports mouthguards with ethylene-vinyl acetate on oral bacteria

Hayashi

Naiki

Murakami

et al. 2022

PeerJ

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Background Sports mouthguards, worn in the oral cavity to prevent sports injuries, are constantly exposed to various microorganisms that cause oral infections. Hence, the optimal cleaning methods for sports mouthguards have been thoroughly examined. In this study, we evaluated the efficiency of cleaning effects with a mouthguard cleaner (MC) on microbial biofilm formation in sports mouthguards in vitro and in vivo. Methods We evaluated the cleaning effects of the discs produced by ethylene-vinyl acetate (EVA) on bacterial biofilms formed by the commensal bacterium Streptococcus oralis, the cariogenic bacterium Streptococcus mutans, and the opportunistic pathogen Staphylococcus aureus in vitro. EVA discs with biofilm were subjected to sterile distilled water (CTRL) and ultrasonic washing (UW), followed by treatment with MC and sodium hypochlorite (NaClO) as positive controls. Thereafter, the viable bacterial cell counts were determined. The bacteria adhering to the sheets before and after the treatment were observed under an electron microscope. The degree of cleanliness and measurement of viable microbial cell counts for total bacteria, Streptococci and Candida, opportunistic fungi, were evaluated on the used experimental sports mouthguards with and without UW and MC treatment in vivo. Results The number of bacterial cells significantly decreased against all the tested biofilm bacteria upon treatment with MC, compared with CTRL and UW. Electron microscopy analysis revealed the biofilm formation by all bacteria on the EVA discs before cleaning. We observed fewer bacteria on the EVA discs treated with MC than those treated with CTRL and UW. Furthermore, the degree of cleanliness of the used experimental sports mouthguards cleaned using MC was significantly higher than that of the CTRL-treated mouthguards. Moreover, the viable microbial cell counts on the used experimental sports mouthguard were considerably lower than those on the CTRL ones. Conclusion The cleaning effect of MC against oral bacteria was more effective than that of UW. MC treatment might have a potential future application as a cleaning method for sports mouthguards to protect athletes from oral infection.

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“…Com o objetivo de se obter um método de limpeza diário de próteses removíveis com maior biocompatibilidade aos tecidos bucais, alguns estudos têm demonstrado a efetividade antimicrobiana de soluções de hipoclorito de sódio e digluconato de clorexidina em baixas concentrações (ALTIERI et al, 2013;DA SILVA, P. M. et al, 2011;MONTAGNER et al, 2009;PELLIZZARO et al, 2012;RODRIGUEZ ACOSTA et al, 2014;WEBB et al, 2005). Entretanto, tais pesquisas não avaliaram o efeito antimicrobiano das soluções de baixas concentrações a longo prazo, simulando a rotina caseira de imersão noturna das próteses pelos pacientes (8h/dia).…”

Section: Introductionunclassified

Efeito antimicrobiano residual e citotoxidade in vitro de resina acrílica para base de prótese após imersão prolongada em agentes de limpeza

Procópio¹

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Candida albicans Adherence to Denture Base Material: Chemical Disinfection and the Effect of Acquired Salivary Pellicle Formation

Abstract: Our results suggest that the presence of saliva might influence the adhesion of C. albicans and improve the effectiveness of methods to reduce fungal adhesion.

Cited by 5 publications

References 47 publications

Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins

Efficacy of Denture Cleansers on Microbial Adherence and Surface Topography of Conventional and CAD/CAM-Processed Denture Base Resins

Effects of cleaning sports mouthguards with ethylene-vinyl acetate on oral bacteria

Efeito antimicrobiano residual e citotoxidade in vitro de resina acrílica para base de prótese após imersão prolongada em agentes de limpeza

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