Butyrivibrio hungateiMB2003 Competes Effectively for Soluble Sugars Released byButyrivibrio proteoclasticusB316Tduring Growth on Xylan or Pectin

Palevich, Nikola; Kelly, William J.; Ganesh, Siva; Rakonjac, Jasna; Attwood, Graeme T.

doi:10.1128/aem.02056-18

Cited by 30 publications

(30 citation statements)

References 79 publications

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“…A feature of MA3014 is the presence of a chromid or secondary chromosome which is also found in other well-characterized Butyrivibrio genomes ( Kelly et al. 2010 ; Palevich, Kelly, Ganesh, et al. 2019 ).…”

Section: Resultsmentioning

confidence: 89%

“…Overall, the coding region comprises 89.77% of the genome, which is typical of rumen Butyrivibrio and Pseudobutyrivibrio genomes sequenced within the Hungate1000 project ( Seshadri et al. 2018 ; Palevich, Kelly, Leahy, et al. 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…MA3014 is predicted to breakdown starch and xylan based on four large (>1,000 aa) cell-associated enzymes ( Kelly et al. 2010 ) shown to be significantly up-regulated in related B. hungatei MB2003 and Butyrivibrio proteoclasticus B316 T cells grown on xylan ( Palevich, Kelly, Ganesh, et al. 2019 ).…”

Section: Resultsmentioning

confidence: 99%

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Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium Pseudobutyrivibrio xylanivorans MA3014 Reveals an Incomplete Glycolytic Pathway

Palevich

Maclean

Kelly³

et al. 2020

Genome Biology and Evolution

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Bacterial species belonging to the genus Pseudobutyrivibrio are important members of the rumen microbiome contributing to the degradation of complex plant polysaccharides. Pseudobutyrivibrio xylanivorans MA3014 was selected for genome sequencing to examine its ability to breakdown and utilize plant polysaccharides. The complete genome sequence of MA3014 is 3.58 Mb, consists of three replicons (a chromosome, chromid and plasmid), has an overall G+C content of 39.6% and encodes 3,265 putative protein-coding genes (CDS). Comparative pan-genomic analysis of all cultivated and currently available P. xylanivorans genomes has revealed a strong correlation of orthologous genes within this rumen bacterial species. MA3014 is metabolically versatile and capable of growing on a range of simple mono- or oligosaccharides derived from complex plant polysaccharides such as pectins, mannans, starch and hemicelluloses, with lactate, butyrate and formate as the principal fermentation end-products. The genes encoding these metabolic pathways have been identified and MA3014 is predicted to encode an extensive range of Carbohydrate-Active enZYmes (CAZymes) with 80 Glycoside Hydrolases (GHs), 28 Carbohydrate Esterases (CEs) and 51 Glycosyl Transferases (GTs), suggesting an important role in solubilization of plant matter in the rumen.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

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Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium Pseudobutyrivibrio xylanivorans MA3014 Reveals an Incomplete Glycolytic Pathway

Palevich

Maclean

Kelly³

et al. 2020

Genome Biology and Evolution

Self Cite

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“…On the other side, Ruminobacter abundance was decreased by a lower level of CO (2%) in low- and medium-roughage rations but increased with a high level of CO (8%) in all rations. Fermentation of polysaccharides by rumen bacteria ( Butyrivibrio and Pseudobutyrivibrio ) produces formic, butyric, and acetate acids in the rumen ( 69 ). The present study showed a higher level of butyric acid with supplementation of CO at 4 and 6% levels in all rations, which is mainly attributed to the increased number of Butyrivibrio species.…”

Section: Discussionmentioning

confidence: 99%

Camelina sativa L. Oil Mitigates Enteric in vitro Methane Production, Modulates Ruminal Fermentation, and Ruminal Bacterial Diversity in Buffaloes

Ebeid

Hassan

et al. 2020

Front. Vet. Sci.

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This study was aimed to evaluate the effects of Camelina sativa oil (CO) on fermentation kinetics and methane (CH 4) production in rations with different roughage (R) to concentrate (C) ratios. Three total mixed rations (TMRs) were used as substrates (R70:C30, R50:C50, and R30:C70) supplemented with different levels of CO (0, 2, 4, 6, and 8% on dry matter basis) in an in vitro batch culture system. The enteric CH 4 production was determined at different times of incubation while fermentation parameters were measured at the end of incubation. Results revealed that CO significantly decreased (P < 0.05) CH 4 production at 48 h in medium (R50:C50) and low-(R30:C70) roughage diets than control. Camelina oil at all levels significantly (P < 0.05) affected ammonia nitrogen (NH 3-N) and microbial protein (MCP) in all rations. Propionate concentration was increased by supplementing 8% CO to R70:C30 TMR, but it decreased with increasing levels of CO for low-and medium-roughage diets. Acetate concentration was significantly (P < 0.05) higher at 4% CO supplementation, but it decreased with 8% CO level in R30:C70 TMR. For all rations, CO decreased (P < 0.001) total bacteria, protozoa, and methanogens. Total fungi counts were affected by CO in all rations, especially with a 6% level in two rations (R30:C70 and R50:C50) and 8% level with high-roughage ration (R70:C30). Supplementation of CO in medium-roughage ration (R50:C50) showed a linear (P < 0.05) decrease in bacterial richness and evenness indices along with Shannon diversity as compared to the control. Moreover, CO also increased Firmicutes to Bacteroidetes ratio in all TMRs more effectively at higher levels. Camelina oil also affected the relative abundance of Prevotella in both low-and medium-roughage diets while increasing the abundance of Ruminobacter and Pseudobutyrivibrio. The present study concluded that CO enhanced fermentation kinetics while decreasing enteric in vitro CH 4 production from fibrous diets. Thus, it may be considered as a potentially effective and environmentally friendly way of mitigating CH 4 emission from livestock.

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“…In response to MP35, we detected a 3-fold increase in the relative abundance of Butyrivibrio , together with a 1.8-fold increase in Pseudobutyrivibrio compared to the control. Bacterial taxa like Firmicutes, Butyrivibrio , and Pseudobutyrivibrio , are important degraders of polysaccharides in the rumen and produce formate, butyrate, and acetate ( 101 ).…”

Section: Discussionmentioning

confidence: 99%

A Mixed Phytogenic Modulates the Rumen Bacteria Composition and Milk Fatty Acid Profile of Water Buffaloes

et al. 2020

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This study was aimed to evaluate the effect of a mixed phytogenic (MP) on rumen bacteria and their potential association with rumen fermentation and milk yield parameters in water buffaloes. Twenty Murrah buffaloes were fed a basal diet (consisting of maize silage, brewers' grains, and concentrate mixture) for 6 weeks supplemented with 0 (control), 15 (MP15), 25 (MP25), and 35 (MP35) g of mixed phytogenic/buffalo per d. The mixed phytogenic contained fennel (seeds), ajwain (seeds), ginger (tubers), Swertia chirata (leaves), Citrullus colocynthis (fruit), turmeric, fenugreek (seeds), Terminalia chebula (fruit), licorice (roots), and Phyllanthus emblica (fruit) in equal quantities. After 2 weeks of adaptation, daily milk yield, and weekly milk composition were recorded. On the last day of the experiment (d 42), rumen contents were collected to determine rumen fermentation parameters and bacterial diversity through 16S rRNA sequencing. Results revealed no change in dry matter intake, milk yield and rumen fermentation parameters except pH, which increased (P = 0.029) in response to MP supplementation. The mixed phytogenic increased (P < 0.01) milk fatty acids (C4 to C14:0) in MP15 only. The milk C16:1 content and its unsaturation index were higher (P < 0.05) in MP35 as compared to the control and other treatments. Furthermore, C18:3n3 was higher (P < 0.05) in the control, MP15, and MP25, as compared to MP35. Supplementation of MP tended to increase (P = 0.095) the Shannon index of bacterial alpha diversity and a difference (P < 0.05) among treatment groups was observed in beta diversity. Feeding MP increased the Firmicutes, Proteobacteria, and Spirochaetes but decreased Bacteroidetes numerically. In addition, the dominant genus Prevotella decreased in all treatment groups while Pseudobutyrivibrio, Butyrivibrio, and Succinivibrioanceae increased numerically in MP25 and MP35. The mixed phytogenic promoted groups of rumen bacteria positively associated with milk and fat yield. Overall, our study revealed 14 positive correlations of rumen bacteria with milk yield and eight with rumen fermentation parameters. Our findings reveal substantial changes in the rumen bacteriome composition and milk fatty acid content in response to MP but these results should be interpreted carefully, as the sample size of our study was relatively small.

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Butyrivibrio hungateiMB2003 Competes Effectively for Soluble Sugars Released byButyrivibrio proteoclasticusB316^Tduring Growth on Xylan or Pectin

Cited by 30 publications

References 79 publications

Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium Pseudobutyrivibrio xylanivorans MA3014 Reveals an Incomplete Glycolytic Pathway

Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium Pseudobutyrivibrio xylanivorans MA3014 Reveals an Incomplete Glycolytic Pathway

Camelina sativa L. Oil Mitigates Enteric in vitro Methane Production, Modulates Ruminal Fermentation, and Ruminal Bacterial Diversity in Buffaloes

A Mixed Phytogenic Modulates the Rumen Bacteria Composition and Milk Fatty Acid Profile of Water Buffaloes

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