<i>Burkholderiaceae</i>
            and Multidrug Resistance Genes Are Key Players in Resistome Development in a Germfree Soil Model

Cao, Yuping; Achmon, Yigal; Yaron, Sima; Siame, Bupe A.; Leung, Ka Yin

doi:10.1128/msystems.00988-21

Cited by 8 publications

(4 citation statements)

References 79 publications

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“…Multiple Drug Resistance ARGs (MDR) dominated the dataset in number (n = 87) and relative abundance, particularly in surface bio lms (79%, 87% and 83% of the reads per million in wood, W-LDPE and LDPE, respectively, vs 36% in water). MDR is known to dominate ARGs in soil microcosms (Cao et al, 2021) as well as in miningimpacted soil samples (Yi et al, 2022), or even in ready-to-eat food (Li et al, 2020). The reported levels of MDR in the literature are in line with our ndings, where MDR accounts for a high proportion of the number of reads and the highest number of genes associated with AMR (Fig.…”

Section: Resultssupporting

confidence: 88%

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Preprint

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Background The widespread nature of plastic pollution has given rise to wide scientific and social concern regarding the capacity of these materials to serve as vectors for pathogenic bacteria and reservoirs for Antimicrobial Resistance Genes (ARG). In- and ex-situ were used to characterise the riverine plastisphere taxonomically and functionally in order to determine whether antibiotics within the water influenced the ARG profiles in these microbiomes and how these compared to those on natural surfaces such as wood and their planktonic counterparts. Results We show that plastics support a taxonomically distinct microbiome containing potential pathogens and ARGs. While the plastisphere was similar to those biofilms that grew on wood, they were distinct from the surrounding water microbiome. Hence, whilst potential opportunistic pathogens (i.e. Pseudomonas aeruginosa, Acinetobacter and Aeromonas) and ARG subtypes (i.e. those that confer resistance to macrolides/lincosamides, rifamycin, sulfonamides, disinfecting agents and glycopeptides) were predominant in all surface-related microbiomes, especially on weathered plastics, a completely different set of potential pathogens (i.e. Escherichia, Salmonella, Klebsiella and Streptococcus) and ARGs (i.e. aminoglycosides, tetracycline, aminocoumarin, fluoroquinolones, nitroimidazole, oxazolidinone and fosfomycin) dominated in the planktonic compartment. Our genome-centric analysis allowed the assembly of 215 Metagenome Assembled Genomes (MAGs), linking ARGs and other virulence-related genes to their host. Interestingly, a MAG belonging to Escherichia –that clearly predominated in water– harboured more ARGs and virulence factors than any other MAG, emphasising the potential virulent nature of these pathogenic-related groups. Finally, ex-situ incubations using environmentally-relevant concentrations of antibiotics increased the prevalence of their corresponding ARGs, but different riverine compartments –including plastispheres– were affected differently by each antibiotic. Conclusions Our results provide insights into the capacity of the riverine plastisphere to harbour a distinct set of potentially pathogenic bacteria and function as a reservoir of ARGs. The environmental impact that plastics pose if they act as a reservoir for either pathogenic bacteria or ARGs is aggravated by the persistence of plastics in the environment due to their recalcitrance and buoyancy. Nevertheless, the high similarities with microbiomes growing on natural co-occurring materials and even more worrisome microbiome observed in the surrounding water highlights the urgent need to integrate the analysis of all environmental compartments when assessing risks and exposure to pathogens and ARGs in anthropogenically-impacted ecosystems.

show abstract

Section: Resultssupporting

confidence: 88%

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Preprint

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show abstract

“…In this context, some of the bacterial taxa found in the plastisphere in the present study have been found to carry ARGs, such as the Burkholderiaceae family, which is a primary carrier of ARGs in situations of high antibiotic concentrations (Cao et al, 2021), such as those occurring in site 2. The genus Acinetobacter, which is part of the core microbiome detected in the PET bottle after 6 months of colonization in site 2, is responsible for the persistence of macrolide resistance ARGs in WWTP effluents, which would also explain the higher relative abundance of ermF in this sites and colonization phase (April et al, 2022).…”

Section: Discussionmentioning

confidence: 68%

Time-course biofilm formation and presence of antibiotic resistance genes on everyday plastic items deployed in river waters

Martínez-Campos

González-Pleiter²,

Rico³

et al. 2023

Journal of Hazardous Materials

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“…Multiple Drug Resistance (MDR) genes dominated the dataset in number ( n = 84) and relative abundance, particularly in surface biofilms (61%, 77% and 66% of the RPKM in wood, W-LDPE and LDPE, respectively , vs. 27% in water). MDR is known to dominate ARGs in soil microcosms [ 31 ] as well as in mining-impacted soil samples [ 158 ], or even in ready-to-eat food [ 79 ]. The reported levels of MDR in the literature are in line with our findings, where MDR accounts for a high proportion of the number of reads and the highest number of genes associated with AMR [ 28 , 134 , 154 ] (Fig.…”

Section: Resultsmentioning

confidence: 99%

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Microbiome

View full text Add to dashboard Cite

Background The widespread nature of plastic pollution has given rise to wide scientific and social concern regarding the capacity of these materials to serve as vectors for pathogenic bacteria and reservoirs for Antimicrobial Resistance Genes (ARG). In- and ex-situ incubations were used to characterise the riverine plastisphere taxonomically and functionally in order to determine whether antibiotics within the water influenced the ARG profiles in these microbiomes and how these compared to those on natural surfaces such as wood and their planktonic counterparts. Results We show that plastics support a taxonomically distinct microbiome containing potential pathogens and ARGs. While the plastisphere was similar to those biofilms that grew on wood, they were distinct from the surrounding water microbiome. Hence, whilst potential opportunistic pathogens (i.e. Pseudomonas aeruginosa, Acinetobacter and Aeromonas) and ARG subtypes (i.e. those that confer resistance to macrolides/lincosamides, rifamycin, sulfonamides, disinfecting agents and glycopeptides) were predominant in all surface-related microbiomes, especially on weathered plastics, a completely different set of potential pathogens (i.e. Escherichia, Salmonella, Klebsiella and Streptococcus) and ARGs (i.e. aminoglycosides, tetracycline, aminocoumarin, fluoroquinolones, nitroimidazole, oxazolidinone and fosfomycin) dominated in the planktonic compartment. Our genome-centric analysis allowed the assembly of 215 Metagenome Assembled Genomes (MAGs), linking ARGs and other virulence-related genes to their host. Interestingly, a MAG belonging to Escherichia –that clearly predominated in water– harboured more ARGs and virulence factors than any other MAG, emphasising the potential virulent nature of these pathogenic-related groups. Finally, ex-situ incubations using environmentally-relevant concentrations of antibiotics increased the prevalence of their corresponding ARGs, but different riverine compartments –including plastispheres– were affected differently by each antibiotic. Conclusions Our results provide insights into the capacity of the riverine plastisphere to harbour a distinct set of potentially pathogenic bacteria and function as a reservoir of ARGs. The environmental impact that plastics pose if they act as a reservoir for either pathogenic bacteria or ARGs is aggravated by the persistence of plastics in the environment due to their recalcitrance and buoyancy. Nevertheless, the high similarities with microbiomes growing on natural co-occurring materials and even more worrisome microbiome observed in the surrounding water highlights the urgent need to integrate the analysis of all environmental compartments when assessing risks and exposure to pathogens and ARGs in anthropogenically-impacted ecosystems.

show abstract

Burkholderiaceae and Multidrug Resistance Genes Are Key Players in Resistome Development in a Germfree Soil Model

Abstract: This is the first study to examine how resistomes develop and evolve using GS. GS can be used to study the colonization and establishment of bacterial communities under antibiotic selection.

Cited by 8 publications

References 79 publications

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Time-course biofilm formation and presence of antibiotic resistance genes on everyday plastic items deployed in river waters

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

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