<i>Burkholderia ginsengisoli</i>bacteraemia: emergence of a novel pathogen

Marks, Laura R.; Dodd, Hema; Russo, Thomas A.; Berenson, Charles S.

doi:10.1136/bcr-2015-213584

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…The genus Paraburkholderia comprises over 70 species that appear mostly restricted to environmental samples. They are isolated from different types of soil and, occasionally, aquatic environments, plant nodules and rhizosphere samples [2][3][4][5][6][7]; yet some Paraburkholderia species, including Paraburkholderia fungorum, Paraburkholderia tropica and Paraburkholderia ginsengisoli, have been isolated from a range of infections in humans and animals [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Genomic Aromatic Compound Degradation Potential of Novel Paraburkholderia Species: Paraburkholderia domus sp. nov., Paraburkholderia haematera sp. nov. and Paraburkholderia nemoris sp. nov.

Vanwijnsberghe

Peeters

Ridder

et al. 2021

IJMS

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We performed a taxonomic and comparative genomics analysis of 67 novel Paraburkholderia isolates from forest soil. Phylogenetic analysis of the recA gene revealed that these isolates formed a coherent lineage within the genus Paraburkholderia that also included Paraburkholderiaaspalathi, Paraburkholderiamadseniana, Paraburkholderiasediminicola, Paraburkholderiacaffeinilytica, Paraburkholderiasolitsugae and Paraburkholderiaelongata and four unidentified soil isolates from earlier studies. A phylogenomic analysis, along with orthoANIu and digital DNA–DNA hybridization calculations revealed that they represented four different species including three novel species and P. aspalathi. Functional genome annotation of the strains revealed several pathways for aromatic compound degradation and the presence of mono- and dioxygenases involved in the degradation of the lignin-derived compounds ferulic acid and p-coumaric acid. This co-occurrence of multiple Paraburkholderia strains and species with the capacity to degrade aromatic compounds in pristine forest soil is likely caused by the abundant presence of aromatic compounds in decomposing plant litter and may highlight a diversity in micro-habitats or be indicative of synergistic relationships. We propose to classify the isolates representing novel species as Paraburkholderia domus with LMG 31832T (=CECT 30334) as the type strain, Paraburkholderia nemoris with LMG 31836T (=CECT 30335) as the type strain and Paraburkholderia haematera with LMG 31837T (=CECT 30336) as the type strain and provide an emended description of Paraburkholderia sediminicola Lim et al. 2008.

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Section: Introductionmentioning

confidence: 99%

Genomic Aromatic Compound Degradation Potential of Novel Paraburkholderia Species: Paraburkholderia domus sp. nov., Paraburkholderia haematera sp. nov. and Paraburkholderia nemoris sp. nov.

Vanwijnsberghe

Peeters

Ridder

et al. 2021

IJMS

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“…Based on advances in whole genomic sequencing and phylogenetic analysis, researchers proposed reorganizing the plant-beneficial-environmental species into new genera, Paraburkholderia and Caballeronia , while the human pathogens would remain within the Burkholderia genus (Dobritsa et al, 2017; Dobritsa and Samadpour, 2016; Eberl and Vandamme, 2016; Sawana et al, 2014). Discrepancies remain, however, as some species possess dual beneficial and pathogenic potential ( Paraburkholderia ginsengisoli and Paraburkholderia tropica ) (Deris et al, 2010; Marks et al, 2016). Thus, the taxonomic organization of these species remains controversial.…”

Section: Introductionmentioning

confidence: 99%

Sequence heterogeneity of the PenA carbapenemase in clinical isolates of Burkholderia multivorans

Becka

Zeiser

Marshall

et al. 2018

Diagnostic Microbiology and Infectious Disease

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Multidrug-resistant gram-negative pathogens are a significant health threat. Burkholderia spp. encompass a complex subset of gram-negative bacteria with a wide range of biological functions that include human, animal, and plant pathogens. The treatment of infections caused by Burkholderia spp. is problematic due to their inherent resistance to multiple antibiotics. The major β-lactam resistance determinant expressed in Burkholderia spp. is a class A β-lactamase of the PenA family. In this study, significant amino acid sequence heterogeneity was discovered in PenA (37 novel variants) within a panel of 48 different strains of Burkholderia multivorans isolated from individuals with cystic fibrosis. Phylogenetic analysis distributed the 37 variants into 5 groups based on their primary amino acid sequences. Amino acid substitutions were present throughout the entire β-lactamase and did not congregate to specific regions of the protein. The PenA variants possessed 5 to 17 single amino acid changes. The N189S and S286I substitutions were most prevalent and found in all variants. Due to the sequence heterogeneity in PenA, a highly conserved peptide (18 amino acids) within PenA was chosen as the antigen for polyclonal antibody production in order to measure expression of PenA within the 48 clinical isolates of B. multivorans. Characterization of the anti-PenA peptide antibody, using immunoblotting approaches, exposed several unique features of this antibody (i.e., detected <500 pg of purified PenA, all 37 PenA variants in B. multivorans, and Pen-like β-lactamases from other species within the Burkholderia cepacia complex). The significant sequence heterogeneity found in PenA may have occurred due to selective pressure (e.g., exposure to antimicrobial therapy) within the host. The contribution of these changes warrants further investigation.

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“…Other HKIII-bearing human pathogens include Kingella kingae, which is linked to septic arthritis, osteomyelitis, spondylodiscitis, meningitis, endocarditis, and lower respiratory tract infections (53), as well as Burkholderia ferrarie and B. fungorum, which are implicated in synovial tissue infection and vaginosis (54,55). Burkholderia ginsengisoli, a plant endosymbiotic bacterium, has recently been reported as a causative agent of bacteremia in a patient with Crohn's disease (56). In a similar fashion, bacteremia caused by the plantassociated bacteria Herbaspirillum huttiense and Acidovorax oryzae were reported in immunocompetent patients (57,58).…”

Section: Class III Histidine Kinasesmentioning

confidence: 99%

Class III Histidine Kinases: a Recently Accessorized Kinase Domain in Putative Modulators of Type IV Pilus-Based Motility

et al. 2017

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Histidine kinases are key components of regulatory systems that enable bacteria to respond to environmental changes. Two major classes of histidine kinases are recognized on the basis of their modular design: classical (HKI) and chemotaxis specific (HKII). Recently, a new type of histidine kinase that appeared to have features of both HKIs and HKIIs was identified and termed HKIII; however, the details of HKIII's relationship to other two classes of histidine kinases, their function, and evolutionary history remain unknown. Here, we carried out genomic, phylogenetic, and protein sequence analyses that allowed us to reveal the unusual evolutionary history of this protein family, formalize its distinctive features, and propose its putative function. HKIIIs are characterized by the presence of sensory domains and the lack of a dimerization domain, which is typically present in all histidine kinases. In addition to a single-domain response regulator, HKIII signal transduction systems utilize CheX phosphatase and, in many instances, an unorthodox soluble chemoreceptor that are usual components of chemotaxis signal transduction systems. However, many HKIII genes are found in genomes completely lacking chemotaxis genes, thus decoupling their function from chemotaxis. By contrast, all HKIIIcontaining genomes also contain pilT, a marker gene for bacterial type IV pilus-based motility, whose regulation is proposed as a putative function for HKIII. These signal transduction systems have a narrow phyletic distribution but are present in many emerging and opportunistic pathogens, thus offering an attractive potential target for future antimicrobial drug design.IMPORTANCE Bacteria adapt to their environment and their hosts by detecting signals and regulating their cellular functions accordingly. Here, we describe a largely unexplored family of signal transduction histidine kinases, called HKIII, that have a unique modular design. While they are currently identified in a relatively short list of bacterial species, this list contains many emerging pathogens. We show that HKIIIs likely control bacterial motility across solid surfaces, which is a key virulence factor in many bacteria, including those causing severe infections. Full understanding of this putative function may help in designing effective drugs against pathogens that will not affect the majority of the beneficial human microbiome.KEYWORDS chemotaxis, evolution, genomics, histidine kinase, motility, pathogens, signal transduction, type IV pili T he transmission of molecular signals in prokaryotes is carried out by the specialized cellular machinery. The simplest signal transduction systems consist of a single protein that is capable of both sensing a signal and directly affecting a cellular

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Burkholderia ginsengisolibacteraemia: emergence of a novel pathogen

Cited by 3 publications

References 11 publications

Genomic Aromatic Compound Degradation Potential of Novel Paraburkholderia Species: Paraburkholderia domus sp. nov., Paraburkholderia haematera sp. nov. and Paraburkholderia nemoris sp. nov.

Genomic Aromatic Compound Degradation Potential of Novel Paraburkholderia Species: Paraburkholderia domus sp. nov., Paraburkholderia haematera sp. nov. and Paraburkholderia nemoris sp. nov.

Sequence heterogeneity of the PenA carbapenemase in clinical isolates of Burkholderia multivorans

Class III Histidine Kinases: a Recently Accessorized Kinase Domain in Putative Modulators of Type IV Pilus-Based Motility

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