<i>Bt</i> rice harbouring <i>cry</i> genes controlled by a constitutive or wound‐inducible promoter: protection and transgene expression under Mediterranean field conditions

Breitler, Jean Christophe; Vassal, Jean-Michel; Catala, Maria Del Mar; Meynard, Donaldo; Marfâ, Victòria; Melé, Enric; Royer, Monique; Murillo, Isabel; Segundo, Blanca San; Guiderdoni, Emmanuel; Messeguer, Joaquima

doi:10.1111/j.1467-7652.2004.00086.x

Cited by 86 publications

(46 citation statements)

References 40 publications

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“…Low copy of the integration of the transgene may be considered as good result, since several studies show the positive correlation between high copy number of transgenes and cytosine methylation mediated silencing (Kohli et al 1996;Lechtenberg et al 2003;Matzke and Matzke 1995). Western blotting also reconfirmed the proper integration and expression of cry1Ab gene in the transgenic plants, however, we found smaller fragments that reacted with Cry1Ab antibody in leaf extracts of transgenic plants could be due to protein degradation by intrinsic leaf proteases (Australia New Zealand Food Authority (ANZFA) 2001) or degradation of protein during sample preparation (Breitler et al 2004;Cheng et al 1998;Fujimoto et al 1993;Lachance et al 2007). CryIAb levels in transgenic plants was estimated to be about 0.1% of total soluble leaf proteins by comparing with Bt protoxin as standard.…”

Section: Discussionsupporting

confidence: 61%

“…This level of protein accumulation of synthetic endotoxin genes under the control of a constitutive promoter was relatively acceptable as compared with those reported in other transgenic plants which typically ranged from 0.002% (Tu et al 2000) to 0.4-0.5% of total soluble protein (Breitler et al 2004;Jansens et al 1997;Koziel et al 1993;Wu et al 2002). The accumulation of the recombinant endotoxin at 0.002-0.005% of total soluble protein has been shown to be sufficient for biological effect (Breitler et al 2004;Vaeck et al 1987). Ghareyazie et al (1997) reported the accumulation of 0.1% synthetic Cry1Ab protein in transgenic rice plants by using the same synthetic cry1Ab gene as used in our study with tissue-specific promoter.…”

Section: Discussionmentioning

confidence: 87%

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Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene

Jafari

Norouzi²,

Malboobi

et al. 2008

Euphytica

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Two diploid sugar beet genotypes of agronomical importance were transformed using Agrobactrium tumefaciens harboring pBI35Scry containing a synthetic cry1Ab gene. Leaf blade with attached shoot bases, a highly regenerative tissue, were used as explant substratum for transformation. PCR screening with cry1Ab-specific primers showed the presence of transgene in more than 50% of the regenerated kanamycin-resistant plants after treatment with the antibiotic. A transformation rate of 8.8-12.2% (depending on genotype) was achieved as revealed by genomic DNA dot blotting. The intact integration of transgene cassette into the genome was furthermore confirmed by Southern blot analysis. The expression of the cry1Ab gene encoding a truncated endotoxin (67 kDa) at about 0.1% of total soluble protein was achieved in the leaves of transgenic plants as shown by Western blot analysis. Bioassays under in vitro conditions with Spodoptera littoralis, one of the most important pests in sugar beet fields, demonstrated enhanced resistance against this pest. The inheritance of the inserted transgene was confirmed in F 1 plants obtained through crossing of T 0 plants with a cytoplasmic male sterile line. Transgenic plants are currently grown in a greenhouse and will be subjected to further bioassay analyses against other lepidopteran pests of sugar beet.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 87%

Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene

Jafari

Norouzi²,

Malboobi

et al. 2008

Euphytica

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“…S. biformis feeds on young plants by lacerating the green tissue of leaves, and outbreaks have often been reported in China, India, and Bangladesh (Nugaliyadde and Heinrichs 1984, Dale 1994, Pathak and Khan 1994. Since the 1990s, numerous rice genotypes expressing various insect-active crystal (Cry) proteins derived from the bacterium Bacillus thuringiensis Berliner (Bt) (referred as Bt rice hereafter) have been developed with resistance to one or more rice lepidopterous pests, the most important of which are the yellow stem borer, Scirpophaga incertula (Walker) (Lepidoptera: Pyralidae), the striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), and the leaffolder, Cnaphalocrocis medinalis (Guené e) (Lepidoptera: Pyralidae) (reviewed by Breitler et al 2004, High et al 2004, Ramesh et al 2004, Riaz et al 2006, Tang et al 2006, Rahman et al 2007. Since the late 1990s, Þeld trials of various Bt rice genotypes expressing Cry1Ab protein (Shu et al 2000;Ye et al 2001aYe et al , 2003, a fusion protein of Cry1Ab/Cry1Ac (Ye et al 2001b), Cry2A, Cry1Ac, and Cry9C , Cry1C (Tang et al 2006), and a fusion protein of Cry1Ac/CpTI (Han et al 2007) have been conducted in China.…”

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Akhtar¹,

Tian²,

Chen³

et al. 2010

Environ Entomol

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Nontarget impacts of six transgenic Bt rice lines (expressing the Cry1Ab or Cry1Ab/ Cry1Ac protein) on the thrips, Stenchaetothrips biformis (Bagnall), attacking the rice seedling and tillering stages, were evaluated under laboratory and Þeld conditions. Laboratory results showed relatively longer larval, pupal development and preoviposition durations of S. biformis. Although it had a shorter oviposition period, female adult longevity and less total laid eggs were found when fed on some tested Bt rice in comparison to non-Bt controls. S. biformis population dynamics in Bt and non-Bt plots were monitored using the plastic bag and beat plate methods. In the Þeld, the temporal patterns of S. biformis population changes were similar between tested Bt rice lines and their respective control; however, the total number of S. biformis individuals collected from the Bt plots were signiÞcantly less or the same, varying from variety to variety, compared with those from the non-Bt plots. ELISA results showed that the Bt insecticidal protein could be transferred from Bt rice to the thrips, and the concentrations of the protein in rice leaves and thrips were not signiÞcantly correlated with some important biological parameters of the thrip. In addition, the potential effects of Bt rice on the abundance of S. biformis candidate predators are also discussed. In conclusion, our results show that the six Bt rice lines assessed may be less preferable host plants to S. biformis at the individual and population levels in comparison to the non-Bt rice plants.

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“…Results of recent field trials in both locations showed significant resistance against target insects, i.e. YSB and RLF (Breitler et al, 2004;Bashir et al, 2005;Mahmood-ur-Rahman et al, 2007, 2014a, 2014bTabashnik et al, 2009) (Table 2). The rice plants were artificially infested ( Figures 1A-1C) with the target insects and their attack was measured quantitatively.…”

Section: Insect-resistant Ricementioning

confidence: 92%

“…Insect-resistant Bt rice has also been produced in Pakistan (Mahmood-ur-Rahman et al, 2007) and in the Mediterranean region (Breitler et al, 2004). Results of recent field trials in both locations showed significant resistance against target insects, i.e.…”

Section: Insect-resistant Ricementioning

confidence: 99%

Genetic improvement of rice for biotic and abiotic stress tolerance

Ansari¹,

Shaheen²,

Bukhari³

et al. 2015

Turk J Bot

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IntroductionRice (Oryza sativa L.) is one of the oldest cultivated crops. It has been cultivated in India and China for several thousands of years (Poehlman and Sleper, 1995). The major cultivated species of rice, Oryza sativa (2n = 2x = 24), originated in southern and southwestern tropical Asia. The other species of cultivated rice, Oryza glaberrima (2n = 2x = 24), is indigenous to the upper valley of the Niger River and it is cultivated only in western tropical Africa. Twenty-three species and 10 recognized genome types (AA, BB, CC, BBCC, CCDD, EE, FF, GG, HHJJ, and HHKK; Gramene: http://www.gramene.org/species/ oryza/rice_taxonomy.html) of Oryza are recognized. Close relatives of O. sativa are the wild perennial species O. rufipogon and the wild annual species O. nivara. Both are diploid weedy species with the AA genome.Rice is an important food crop and it needs continuous improvement due to the continuous increase in population. The major objectives to improve rice crops using biotechnological techniques include: 1) high yield potential, 2) early maturity, 3) resistance to lodging and shattering, 4) resistance to stress environments, 5) disease resistance, 6) insect resistance, 7) grain quality, and 8) enhancement of nutritional components. Economic importance of riceRice (Oryza sativa L.) is the most important crop of world. About 90% of the world's rice is grown in China, India, Pakistan, Japan, Korea, Southeast Asia, and other adjacent areas (USDA, 2014). Outside of Asia, Brazil and the United States produce the largest amounts of rice (Poehlman and Sleper, 1995). Rice is the staple food for over one-half of the world's people (FAO, 2008). In Pakistan, rice is a highly valued food crop and it is also a major export item. It accounts for 3.2% of the total value added in agriculture and 0.7% of the GDP. Area sown for rice is estimated at 2.891 × 10 6 ha and the production was 7.005 × 10 6 t in -2015 (Ministry of Finance, 2015. Rice is cultivated in diversified climatic conditions of Pakistan. Basmati rice (Indica) is grown in the traditional rice-growing belt of Punjab Province. In Swat, in high altitude alpine valleys, temperate Japonica rice is grown. In the south of Khyber Pakhtunkhwa, Sindh and Baluchistan provinces, IRRItype long-grain heat-tolerant tropical rice is mainly grown. Transformation of rice cropsThe world will need about 25% more rice by the year 2030 to meet the estimated demand of an increasing global population (Wani and Sah, 2014). One way to meet this challenge is to grow rice on more area, which is difficult due to increasing urbanization and escalating population in Abstract: Rice (Oryza sativa L.) is among the most important food crops that provide a staple food for nearly half of the world's population. Rice crops are prone to various types of stresses, both biotic and abiotic. Biotic stresses include insect pests, fungus, bacteria, viruses, and herbicide toxicity. Among abiotic stresses, drought, cold, and salinity are also well studied in rice. Various genes have been identified,...

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Bt rice harbouring cry genes controlled by a constitutive or wound‐inducible promoter: protection and transgene expression under Mediterranean field conditions

Cited by 86 publications

References 40 publications

Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene

Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Genetic improvement of rice for biotic and abiotic stress tolerance

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