<i>Bt</i> rice could provide ecological resistance against nontarget planthoppers

Wang, Xingyun; Liu, Qingsong; Meissle, Michael; Peng, Yufa; Wu, Kongming; Romeis, Jörg; Li, Yunhe

doi:10.1111/pbi.12911

Cited by 31 publications

(36 citation statements)

References 39 publications

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“…Actually, improving the efficiency of controlling pests with piercing-sucking mouthparts is still an important task. A recent study showed that less caterpillar damage in Bt rice attracts fewer planthoppers, implying possible ecological resistance against BPH (Wang et al, 2018). Whether transgenic miR-14 rice also has this kind of resistance to nontarget planthoppers requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Xiao

Sun

et al. 2018

Plant Biotechnology Journal

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Rice stem borer (RSB, Chilo suppressalis) is an insect pest that causes huge economic losses every year. Control efforts rely heavily on chemical insecticides, which leads to serious problems such as insecticide resistance, environment pollution, and food safety issues. Therefore, developing alternative pest control methods is an important task. Here, we identified an insect-specific microRNA, miR-14, in RSB, which was predicted to target Spook (Spo) and Ecdysone receptor (EcR) in the ecdysone signalling network. In-vitro dual luciferase assays using HEK293T cells confirmed the interactions of Csu-miR-14 with CsSpo and with CsEcR. Csu-miR-14 exhibited high levels of expression at the end of each larval instar stage, and its expression was negatively correlated with the expression of its two target genes. Overexpression of Csu-miR-14 at the third day of the fifth instar stage led to high mortality and developmental defects in RSB individuals. We produced 35 rice transformants to express miR-14 and found that three lines had a single copy with highly abundant miR-14 mature transcripts. Feeding bioassays using both T and T generations of transgenic miR-14 rice indicated that at least one line (C#24) showed high resistance to RSB. These results indicated that the approach of miRNAs as targets has potential for improving pest control methods. Moreover, using insect-specific miRNAs rather than protein-encoding genes for pest control may prove benign to non-insect species, and thus is worthy of further exploration.

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Section: Discussionmentioning

confidence: 99%

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Xiao

Sun

et al. 2018

Plant Biotechnology Journal

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“…This is necessary because non-Bt crop refuge has been proposed as a promising strategy for delaying the resistance development towards Bt crop (Gould, 2000;Tabashnik and Carriere, 2017). However, previous studies reported that planthoppers move from Bt to adjacent non-Bt rice fields (Chen et al 2003a;Wang et al, 2018) and that fungal diseases and non-target pests still required to be controlled with pesticides application when full yield is expected to be achieved (Wang et al, 2010). This indicates even if the cultivation of Bt rice cultivation has a great potential to reduce the use of broad-spectrum chemical insecticides (High et al, 2004), pesticides application in Bt rice is still required.…”

Section: More Noticeable Change In Soil Nematodes Community With Timementioning

confidence: 99%

“…Empirical evidence supported Bt rice can result in an evidently decreased application of pesticides, and thus benefit human health and the environment (Huang et al, 2005;Chen et al, 2011). However, the Cry1Ab/Ac protein, which was continuously produced within plant tissue of Bt-rice, could be released through root exudates during growth and persistent in the rhizosphere in paddy soils (Wang et al, 2018). Therefore, it might affect the activity, structure, diversity of soil fauna, and interactions among components of soil food webs, which in turn would influence soil fertility and plant productivity.…”

Section: Introductionmentioning

confidence: 99%

Effects of Pest Management Practices on Soil Nematode Abundance, Diversity, Metabolic Footprint and Community Composition Under Paddy Rice Fields

et al. 2020

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The wide-scale adoption of transgenic crops has aroused public concern towards potential impacts to the ecological services of soil fauna, such as soil nematodes. However, few studies has examined whether the cultivation of transgenic rice would pose greater threats to soil nematode community and associated ecological functions than insecticides application. Moreover, what are determinants of soil nematode community in paddy fields remains unclear. During a 3-year field study, rhizosphere soil samples of transgenic-Bt rice, its counterpart non-Bt parental rice and not-Bt rice with insecticides application were taken at four times in the rice developmental cycle using a random block design with three replications for each treatment. We hypothesized that the effects of pest management practice on soil nematode abundance and metabolic footprint change with trophic group and sampling time. We also predicted there were significant differences in structure and composition of soil nematode community across the three treatments examined and sampling times. In agreement with our expectation, the effects of pest management practice on nematode abundance and metabolic footprints depend on trophic group and sampling time. However, pest management practice exerted no apparent effect on nematode diversity and community composition. Soil nutrient availability and C:N molar ratio are the primary regulating factor of soil nematode community in rice paddy fields. In conclusion, our findings implied that changes in abundance, diversity, metabolic footprints associated with the crop growth stage overweighed the application of Bt rice and insecticides. The cultivation of Bt rice Huahui-1 exerted no measurable adverse effect on soil nematode community in rhizosphere soil over 3 years of rice cropping.

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“…The caterpillar damage induced the release of attractive volatiles for BPH, and BPH therefore preferred to feed on the non‐ Bt rice than on Bt rice plants. The less caterpillar damage‐associated resistance is considered a first example of ecological resistance of Bt plants to rice planthoppers …”

Section: Biological Control Agents Of Planthoppersmentioning

confidence: 99%

“…The less caterpillar damage-associated resistance is considered a first example of ecological resistance of Bt plants to rice planthoppers. 7 In addition to using Bacillus spp. and their gene products or insect pathogenic fungi such as Beauveria bassiana sensu lato isolate NJBb2101 as biological control agents, 8 the utilization and conservation of natural enemies and enhancement of their activities are a basic measure for biological control of planthoppers, and enhancement of the activities of natural enemies is currently one of the most attractive alternatives to biological control of planthoppers.…”

Section: Biological Control Agents Of Planthoppersmentioning

confidence: 99%

Current understanding of the molecular players involved in resistance to rice planthoppers

Yang

Zhang

2019

Pest Management Science

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Rice planthoppers are the most widespread and destructive pest of rice. Planthopper control depends greatly on the understanding of molecular players involved in resistance to planthoppers. This paper summarizes the recent progress in the understanding of some molecular players involved in resistance to planthoppers and the mechanisms involved. Recent researches showed that host‐plant resistance is the most promising sustainable approach for controlling planthoppers. Planthopper‐resistant varieties with a host‐plant resistance gene have been released for rice products. Integrated planthopper management is a proposed strategy to prolong the durability of host‐plant resistance. Bacillus spp. and their gene products or insect pathogenic fungi have great potential for application in the biological control of planthoppers. Enhancement of the activity of the natural enemies of planthoppers would be more cost‐effective and environmentally friendly. Various molecular processes regulate rice–planthopper interactions. Rice encounters planthopper attacks via transcription factors, secondary metabolites, and signaling networks in which phytohormones have central roles. Maintenance of cell wall integrity and lignification act as physical barriers. Indirect defenses of rice are regulated via chemical elicitors, honeydew‐associated elicitor, amendment with silicon and biochar, and salivary protein of BPH as elicitor or effector. Further research directions on planthopper control and rice defense against planthoppers are also put forward. © 2019 Society of Chemical Industry

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Bt rice could provide ecological resistance against nontarget planthoppers

Cited by 31 publications

References 39 publications

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Effects of Pest Management Practices on Soil Nematode Abundance, Diversity, Metabolic Footprint and Community Composition Under Paddy Rice Fields

Current understanding of the molecular players involved in resistance to rice planthoppers

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