<i>Bryophyllum pinnatum</i> – Reverse Engineering of an Anthroposophic Herbal Medicine

Hamburger, Matthias; Potterat, Olivier; Fürer, Karin; Simões‐Wüst, Ana Paula; Mandach, Ursula von

doi:10.1177/1934578x1701200847

Cited by 11 publications

(13 citation statements)

References 39 publications

(48 reference statements)

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“…; family Crassulaceae] is a perennial succulent plant that grows widely in tropical and subtropical areas. In Europe, B. pinnatum started to be used in anthroposophic medicine [14] as treatment of preterm labour [15, 16]. Retrospective analyses of the clinical practice revealed good efficacy of B. pinnatum preparations in this indication [15, 16], which was corroborated by a matched pair study [17].…”

Section: Introductionmentioning

confidence: 94%

“…In Europe, B. pinnatum started to be used in anthroposophic medicine [14] as treatment of preterm labour [15, 16]. Retrospective analyses of the clinical practice revealed good efficacy of B. pinnatum preparations in this indication [15, 16], which was corroborated by a matched pair study [17]. Results of a prospective randomised trial on acute tocolysis are in line with good efficacy, but have to be interpreted with caution since - due to poor recruitment rate - the study was discontinued before completion [18].…”

Section: Introductionmentioning

confidence: 99%

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Bryophyllum pinnatum enhances the inhibitory effect of atosiban and nifedipine on human myometrial contractility: an in vitro study

Santos

Haslinger

Mennet

et al. 2019

BMC Complement Altern Med

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BackgroundThe herbal medicine Bryophyllum pinnatum has been used as a tocolytic agent in anthroposophic medicine and, recently, in conventional settings alone or as an add-on medication with tocolytic agents such as atosiban or nifedipine. We wanted to compare the inhibitory effect of atosiban and nifedipine on human myometrial contractility in vitro in the absence and in the presence of B. pinnatum press juice (BPJ).MethodsMyometrium biopsies were collected during elective Caesarean sections. Myometrial strips were placed under tension into an organ bath and allowed to contract spontaneously. Test substances alone and at concentrations known to moderately affect contractility in this setup, or in combination, were added to the organ bath, and contractility was recorded throughout the experiments. Changes in the strength (measured as area under the curve (AUC) and amplitude) and frequency of contractions after the addition of all test substances were determined. Cell viability assays were performed with the human myometrium hTERT-C3 and PHM1–41 cell lines.ResultsBPJ (2.5 μg/mL), atosiban (0.27 μg/mL), and nifedipine (3 ng/mL), moderately reduced the strength of spontaneous myometrium contractions. When BPJ was added together with atosiban or nifedipine, inhibition of contraction strength was significantly higher than with the tocolytics alone (p = 0.03 and p < 0.001, respectively). In the case of AUC, BPJ plus atosiban promoted a decrease to 48.8 ± 6.3% of initial, whereas BPJ and atosiban alone lowered it to 70.9 ± 4.7% and to 80.9 ± 4.1% of initial, respectively. Also in the case of AUC, BPJ plus nifedipine promoted a decrease to 39.9 ± 4.6% of initial, at the same time that BPJ and nifedipine alone lowered it to 78.9 ± 3.8% and 71.0 ± 3.4% of initial. Amplitude data supported those AUC data. The inhibitory effects of BPJ plus atosiban and of BPJ plus nifedipine on contractions strength were concentration-dependent. None of the test substances, alone or in combination, decreased myometrial cell viability.ConclusionsBPJ enhances the inhibitory effect of atosiban and nifedipine on the strength of myometrial contractions, without affecting myometrium tissue or cell viability. The combination treatment of BPJ with atosiban or nifedipine has therapeutic potential.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Bryophyllum pinnatum enhances the inhibitory effect of atosiban and nifedipine on human myometrial contractility: an in vitro study

Santos

Haslinger

Mennet

et al. 2019

BMC Complement Altern Med

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“…In Europe, BP started to be used at the beginning of the 20th century in anthroposophic medicine, a form of holistic medicine with an integrative approach . The indications for which BP is often used within this type of medicine and more recently also in conventional settings include preterm labor, overactive bladder, and sleep disorders . Several retrospective and a few prospective studies on the treatment of preterm contractions and of overactive bladder with BP preparations have been performed and suggest good effectiveness and very good safety/tolerability.…”

Section: Discussionmentioning

confidence: 99%

“…Preparations of Bryophyllum pinnatum (BP) leaves have been proposed to possess both sedative and muscle relaxant properties . Moreover, prospective observational trials performed with pregnant women and cancer patients revealed good effectiveness in the treatment of sleep disorders with BP 50% chewable tablets (each 350 mg tablet corresponds to 170 mg of leave press juice, dried down to 17 mg by mixing with lactose; 100 mg dried BP matter in 1 g).…”

Section: Introductionmentioning

confidence: 99%

Bryophyllum pinnatum in the treatment of restless legs syndrome: A case series documented with polysomnography

Manitius

Flügel

Steinlein

et al. 2019

Clinical Case Reports

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Key Clinical Message Restless legs syndrome may seriously affect patients' sleep and quality of life, but established pharmacological therapy can often have severe side effects. Therefore, new therapeutic approaches such as well‐tolerated preparations from the medicinal plant Bryophyllum pinnatum should be considered as alternatives. Their sedative and spasmolytic properties might contribute to improve patients' condition.

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“…In Europe, the use of remedies prepared from B. pinnatum leaves was limited for a long time to anthroposophic medicine. Initially, they were used for the treatment of various hyperactivity disorders [3,4], and only in the 1970s was B. pinnatum introduced by the German gynaecologist Werner Hassauer (1928Hassauer ( -1993 as a routine treatment of preterm labour [5]. In Switzerland, B. pinnatum is used in gynaecology and obstetrics against premature contractions, restlessness, and overactive bladder [6].…”

Section: Introductionmentioning

confidence: 99%

A Bufadienolide-Enriched Fraction of Bryophyllum pinnatum Inhibits Human Myometrial Contractility In Vitro

et al. 2018

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Bryophyllum pinnatum has been used since the 1970s to prevent premature labour, first in anthroposophic hospitals and, more recently, also in the main Swiss perinatal centres. However, it is not known which compounds in B. pinnatum leaves contribute to the tocolytic effect. Here we studied the effects of a flavonoid-enriched fraction, the corresponding flavonoid aglycon mixture, a bufadienolide-enriched fraction, and B. pinnatum leaf press juice on human myometrial contractility in vitro. The strength (area under the curve and amplitude) and frequency of contractions were recorded using strips of human myometrium mounted in an organ bath system. Cell viability assays were performed with the human myometrium hTERT-C3 and PHM1 – 41 cell lines. Repeated addition of the flavonoid-enriched fraction, flavonoid aglycon mixture, bufadienolide-enriched fraction, or B. pinnatum leaf press juice led to a progressive decrease of contraction strength, without jeopardising the vitality of myometrium strips. The bufadienolide-enriched fraction was the most active, since 1 µg/mL of the bufadienolide-enriched fraction lowered the area under the curve to 40.1 ± 11.8% of the initial value, whereas 150 µg/mL of the flavonoid-enriched fraction, 6.2 µg/mL of the flavonoid aglycon mixture, and 10 µg/mL of the B. pinnatum leaf press juice were required to achieve comparable inhibition. A progressive increase of contraction frequency was observed, except in the case of the flavonoid aglycon mixture, which did not affect frequency. None of the test substances decreased myometrial cell viability, even at concentrations of 500 µg/mL of the flavonoid-enriched fraction, 40 µg/mL of the flavonoid aglycon mixture, 3.8 µg/mL of the bufadienolide-enriched fraction, and 75 µg/mL of the B. pinnatum leaf press juice, i.e., higher than those used in the myometrium experiments. Given the concentrations of flavonoids in the flavonoid-enriched fraction and B. pinnatum leaf press juice, and of bufadienolides in the bufadienolide-enriched fraction and B. pinnatum leaf press juice, it appears that bufadienolides may be mainly responsible for the relaxant effect.

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Bryophyllum pinnatum – Reverse Engineering of an Anthroposophic Herbal Medicine

Cited by 11 publications

References 39 publications

Bryophyllum pinnatum enhances the inhibitory effect of atosiban and nifedipine on human myometrial contractility: an in vitro study

Bryophyllum pinnatum enhances the inhibitory effect of atosiban and nifedipine on human myometrial contractility: an in vitro study

Bryophyllum pinnatum in the treatment of restless legs syndrome: A case series documented with polysomnography

A Bufadienolide-Enriched Fraction of Bryophyllum pinnatum Inhibits Human Myometrial Contractility In Vitro

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