<i>Brucella ovis</i>vaccination of rams

Buddle, M. B.; Calverley, F.K.; Boyes, Betty W.

doi:10.1080/00480169.1963.33504

Cited by 17 publications

(6 citation statements)

References 10 publications

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“…However, both vaccines have the disadvantages of inducing OPS-specific antibody responses that interfere with serological diagnosis of disease (7,24,28,40,46), of causing abortion in vaccinated animals under some circumstances (19,27), and of being pathogenic for humans (6,53). Alternative vaccines to strains 19 and Rev 1 have been sought for many years, with limited success (1,8,15,18,32,37,47).…”

mentioning

confidence: 99%

Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis

et al. 1994

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Vaccination of BALB/c mice with live BruceUla abortus RB51, a stable rough mutant, produced protection against challenge with virulent strains ofBruceUla abortus, BruceUa melitensis, and Brucella ovis. Passive-transfer experiments indicated that vaccinated mice were protected against B. abortus 2308 through cell-mediated immunity, against B. ovis PA through humoral immunity, and against B. melitensis 16M through both forms of immunity. Live bacteria were required for the induction of protective cell-mediated immunity; vaccination with whole killed cells of strain RB51 failed to protect mice against B. abortus 2308 despite development of good delayed-type hypersensitivity reactions. Protective antibodies against the heterologous species were generated in vaccinated mice primarily through anamnestic responses following challenge infections.

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confidence: 99%

Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis

et al. 1994

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“…Reduced fertility of rams is the most serious consequence of this disease (29), although abortion also occurs (19,27,47). The principal vaccine used against ovine brucellosis has been REV 1, a live attenuated strain of B. melitensis (5,6,8,15,28). Although REV 1 is effective when administered to lambs (5), it is less effective in vaccination of adult animals (53).…”

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confidence: 99%

“…Moreover, this vaccine is virulent for humans (15), and its use is prohibited in the United States due to the possibility of transmitting B. melitensis. Bacterins of B. ovis in several adjuvants have been tested in rams and have provided protection against infection and disease to 50% or more of the vaccinated animals (1,6,8,26,28,49,50). However, bacterins were subject to several disadvantages including the need for multiple vaccinations, the development of lesions at the site of injection, and the induction of immune responses that interfered with the interpretation of diagnostic tests (30,50,58).…”

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Comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis

et al. 1989

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938-942, 1986). The distribution and antigenic relatedness of proteins in HS extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting for 41 strains of B. ovis and 26 strains of BruceUa melitensis of diverse geographic origin. Five major groups of proteins were identified in HS extracts of B. ovis that had been freed of rough lipopolysaccharide: proteins of 43 kilodaltons (kDa), group A (25.5 to 32.0 kDa), group B (21.5 to 22.5 kDa); group C (18.0 to 19.5 kDa), and group D (13.0 to 15.5 kDa). Group A, B, C, and D proteins were also present in blebs. The profiles of proteins in HS extracts or blebs from strains of both Brucella species were very similar. Cross-reactions were demonstrated among HS extracts and blebs of all strains tested in immunoblots performed with an antiserum against the HS extract of a reference strain of B. ovis. Evidence was also provided of an antigenic relationship between group 3 proteins of the outer membrane and some of the proteins in groups A, B, and C. The conservation of these antigens and their immunogenicity in infected animals provide promise that they may serve as components of an effective subcellular vaccine for ovine brucellosis.

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“…(2)(3) Subsequently, it was shown that two doses of the inactivated vaccine given subcutaneously a few weeks apart produced a comparable resistance. (4) (5) However, this later method fell out of favour because of the unsightly lesions which developed at the site of vaccination in the neck.…”

Section: Introductionmentioning

confidence: 96%

The serological response of rams to four methods of vaccination againstBrucella ovisinfection

Bailey

West²,

Bruère³

1987

New Zealand Veterinary Journal

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A comparative study was made of the serological response of rams to an inactivated Brucella ovis saline in-oil vaccine administered either once or twice by either the subcutaneous or the intraperitoneal route. The serological response of rams to two spaced doses of vaccine was more consistent and more persistent than when a single dose of vaccine was used. The rise in titre was more rapid and the final titre of greater magnitude when the subcutaneous route of administration was used in comparison with the intraperitoneal route. On a serological basis, the most satisfactory vaccination technique investigated consisted of two subcutaneous injections of vaccine administered ten weeks apart. The least satisfactory technique was a single intraperitoneal injection.

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Brucella ovisvaccination of rams

Cited by 17 publications

References 10 publications

Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis

Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis

Comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis

The serological response of rams to four methods of vaccination againstBrucella ovisinfection

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