<i>Brittle Culm 1</i>Encodes a COBRA-Like Protein Involved in Secondary Cell Wall Cellulose Biosynthesis in Sorghum

Li, Pan; Liu, Yanrong; Tan, Wenqing; Chen, Jun; Zhu, Meiying; Lv, Ya; Yu, Shaohua; Zhang, Wanjun; Cai, Hongwei

doi:10.1093/pcp/pcy246

Cited by 36 publications

(28 citation statements)

References 59 publications

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“…More members of COBL are found in dicots, as several duplications occurred after the separation of dicots and monocots during evolution [102]. Recently, it has been demonstrated that a Sorghum bicolor COBL protein is linked to cellulose biosynthesis in the secondary wall, affecting plant mechanical strength [103], and this provided evidence of a cellulose-related role for this protein family in grasses. Overall, the LRR domain-containing proteins are conserved in both dicots and monocots [104].…”

Section: Functional Class Distribution In Monocotsmentioning

confidence: 99%

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Calderan-Rodrigues

Fonseca

Moraes

et al. 2019

IJMS

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Plant cell walls mostly comprise polysaccharides and proteins. The composition of monocots’ primary cell walls differs from that of dicots walls with respect to the type of hemicelluloses, the reduction of pectin abundance and the presence of aromatic molecules. Cell wall proteins (CWPs) differ among plant species, and their distribution within functional classes varies according to cell types, organs, developmental stages and/or environmental conditions. In this review, we go deeper into the findings of cell wall proteomics in monocot species and make a comparative analysis of the CWPs identified, considering their predicted functions, the organs analyzed, the plant developmental stage and their possible use as targets for biofuel production. Arabidopsis thaliana CWPs were considered as a reference to allow comparisons among different monocots, i.e., Brachypodium distachyon, Saccharum spp. and Oryza sativa. Altogether, 1159 CWPs have been acknowledged, and specificities and similarities are discussed. In particular, a search for A. thaliana homologs of CWPs identified so far in monocots allows the definition of monocot CWPs characteristics. Finally, the analysis of monocot CWPs appears to be a powerful tool for identifying candidate proteins of interest for tailoring cell walls to increase biomass yield of transformation for second-generation biofuels production.

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Section: Functional Class Distribution In Monocotsmentioning

confidence: 99%

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Calderan-Rodrigues

Fonseca

Moraes

et al. 2019

IJMS

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“…Mature “XMQ” and “JQ” seeds were observed by scanning electron microscopy, which was carried out as previously described [ 40 ]. For paraffin section analysis, “XMQ” and “JQ” seeds of four different development stages were used, and the treatment was performed as previously described [ 40 ]. The hull ditch thickness of “XMQ” and “JQ” were measured using CaseViewer software based on the paraffin section result.…”

Section: Methodsmentioning

confidence: 99%

Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)

et al. 2020

BMC Plant Biol

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Background Tartary buckwheat has gained popularity in the food marketplace due to its abundant nutrients and high bioactive flavonoid content. However, its difficult dehulling process has severely restricted its food processing industry development. Rice-tartary buckwheat, a rare local variety, is very easily dehulled, but the cellular, physiological and molecular mechanisms responsible for this easy dehulling remains largely unclear. Results In this study, we integrated analyses of the comparative cellular, physiological, transcriptome, and gene coexpression network to insight into the reason that rice-tartary buckwheat is easy to dehull. Compared to normal tartary buckwheat, rice-tartary buckwheat has significantly brittler and thinner hull, and thinner cell wall in hull sclerenchyma cells. Furthermore, the cellulose, hemicellulose, and lignin contents of rice-tartary buckwheat hull were significantly lower than those in all or part of the tested normal tartary buckwheat cultivars, respectively, and the significant difference in cellulose and hemicellulose contents between rice-tartary buckwheat and normal tartary buckwheat began at 10 days after pollination (DAP). Comparative transcriptome analysis identified a total of 9250 differentially expressed genes (DEGs) between the rice- and normal-tartary buckwheat hulls at four different development stages. Weighted gene coexpression network analysis (WGCNA) of all DEGs identified a key module associated with the formation of the hull difference between rice- and normal-tartary buckwheat. In this specific module, many secondary cell wall (SCW) biosynthesis regulatory and structural genes, which involved in cellulose and hemicellulose biosynthesis, were identified as hub genes and displayed coexpression. These identified hub genes of SCW biosynthesis were significantly lower expression in rice-tartary buckwheat hull than in normal tartary buckwheat at the early hull development stages. Among them, the expression of 17 SCW biosynthesis relative-hub genes were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Conclusions Our results showed that the lower expression of SCW biosynthesis regulatory and structural genes in rice-tartary buckwheat hull in the early development stages contributes to its easy dehulling by reducing the content of cell wall chemical components, which further effects the cell wall thickness of hull sclerenchyma cells, and hull thickness and mechanical strength.

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“…In addition, the encoded proteins and functions of the 20 genes were annotated, and many were associated with fiber development (Table 4). Gh_ D11G1626 encoded a COBRA-like protein 4, and expressed in the fiber SCW biosynthesis stages; the function of COBRA-like protein has been reported in sorghum and rice, which were involved in SCW cellulose biosynthesis (Dai et al 2011;Li et al 2019;Sato et al 2010), so Gh_D11G1626 is probably involved in the SWC biosynthesis in cotton. Gh_D13G1900 and Gh_ A01G1474 encoded WAT1-related proteins, and showed specifically high expression level at the fiber SCW biosynthesis stages; the WAT1-related protein was probably related to high fiber yield in cotton (Liu et al 2018a).…”

Section: Candidate Genes Are Probably Involved In the Development Of mentioning

confidence: 99%

Identification of candidate genes controlling fiber quality traits in upland cotton through integration of meta-QTL, significant SNP and transcriptomic data

et al. 2020

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Background Meta-analysis of quantitative trait locus (QTL) is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies. The combination of meta-QTL intervals, significant SNPs and transcriptome analysis has been widely used to identify candidate genes in various plants. Results In our study, 884 QTLs associated with cotton fiber quality traits from 12 studies were used for meta-QTL analysis based on reference genome TM-1, as a result, 74 meta-QTLs were identified, including 19 meta-QTLs for fiber length; 18 meta-QTLs for fiber strength; 11 meta-QTLs for fiber uniformity; 11 meta-QTLs for fiber elongation; and 15 meta-QTLs for micronaire. Combined with 8 589 significant single nucleotide polymorphisms associated with fiber quality traits collected from 15 studies, 297 candidate genes were identified in the meta-QTL intervals, 20 of which showed high expression levels specifically in the developing fibers. According to the function annotations, some of the 20 key candidate genes are associated with the fiber development. Conclusions This study provides not only stable QTLs used for marker-assisted selection, but also candidate genes to uncover the molecular mechanisms for cotton fiber development.

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Brittle Culm 1Encodes a COBRA-Like Protein Involved in Secondary Cell Wall Cellulose Biosynthesis in Sorghum

Cited by 36 publications

References 59 publications

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa

Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)

Identification of candidate genes controlling fiber quality traits in upland cotton through integration of meta-QTL, significant SNP and transcriptomic data

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