<i>Brevundimonas</i>

Vancanneyt, Marc; Segers, Paul; Abraham, Wolf‐Rainer; Vos, Paul De

doi:10.1002/9781118960608.gbm00791

Cited by 5 publications

(3 citation statements)

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“…These parameters are all intrinsically related to each other and, as suggested by several authors, this could imply a cause-effect mechanism ( Taha et al, 2012 ; Peluso et al, 2013 ; Ribas-Maynou and Benet, 2019 ). The catalase activity and carotenoid production observed in Brevundimonas could contribute to the reduction of seminal oxidative stress ( Vancanneyt et al, 2015 ; Liu et al, 2020 ), through a decrease in cell membrane damage caused by lipid peroxidation. If this peroxidation occurs, DNA would be more exposed to oxidative stress, and extensive DNA damage would be generated.…”

Section: Discussionmentioning

confidence: 99%

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Garcia-Segura

Rey

Closa

et al. 2022

Front. Cell Dev. Biol.

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The development of new biomarkers for human male infertility is crucial to improve the diagnosis and the prognosis of this disease. Recently, seminal microbiota was shown to be related to sperm quality parameters, suggesting an effect in human fertility and postulating it as a biomarker candidate. However, its relationship to sperm DNA integrity has not been studied yet. The aim of the present study is to characterize the seminal microbiota of a western Mediterranean population and to evaluate its relationship to sperm chromatin integrity parameters, and oxidative stress. For that purpose, 14 samples from sperm donors and 42 samples from infertile idiopathic patients were obtained and were analyzed to assess the composition of the microbiota through full-length 16S rRNA gene sequencing (Illumina MiSeq platform). Microbial diversity and relative abundances were compared to classic sperm quality parameters (macroscopic semen parameters, motility, morphology and concentration), chromatin integrity (global DNA damage, double-stranded DNA breaks and DNA protamination status) and oxidative stress levels (oxidation-reduction potential). The seminal microbiota observed of these samples belonged to the phyla Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. The most abundant genera were Finegoldia, Peptoniphilus, Anaerococcus, Campylobacter, Streptococcus, Staphylococcus, Moraxella, Prevotella, Ezakiella, Corynebacterium and Lactobacillus. To our knowledge, this is the first detection of Ezakiella genus in seminal samples. Two clusters of microbial profiles were built based on a clustering analysis, and specific genera were found with different frequencies in relation to seminal quality defects. The abundances of several bacteria negatively correlate with the sperm global DNA fragmentation, most notably Moraxella, Brevundimonas and Flavobacterium. The latter two were also associated with higher sperm motility and Brevundimonas additionally with lower oxidative-reduction potential. Actinomycetaceae, Ralstonia and Paenibacillus correlated with reduced chromatin protamination status and increased double-stranded DNA fragmentation. These effects on DNA integrity coincide in many cases with the metabolism or enzymatic activities of these genera. Significant differences between fertile and infertile men were found in the relative presence of the Propionibacteriaceae family and the Cutibacterium, Rhodopseudomonas and Oligotropha genera, which supports its possible involvement in male fertility. Our findings sustain the hypothesis that the seminal microbiome has an effect on male fertility.

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Section: Discussionmentioning

confidence: 99%

Seminal Microbiota of Idiopathic Infertile Patients and Its Relationship With Sperm DNA Integrity

Garcia-Segura

Rey

Closa

et al. 2022

Front. Cell Dev. Biol.

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“…LVF2 T also presented its competitive advantage at extreme temperatures such as 4 C. It showed detectable growth after nine days, whereas LVF1 T required 16 days (data not shown). The data regarding growth in different NaCl concentrations correlate well with those known from literature for this genus [2,78] and are in good agreement with parameters frequently observed in environments from which both strains originated [78].…”

Section: Discussionsupporting

confidence: 88%

Structure and function of bacterial viruses and viral communities

Friedrich¹

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Bacterial viruses, known as bacteriophages or phages, are the most abundant biological entities on the planet and the least studied in terms of abundance and diversity. Searching the sequence databases of viral genomes, one becomes the impression that most of the viral sphere consists of dsDNA bacteriophages. First objective of the studies was to verify whether this is true or a methodical artefact of our usual approach of assessing the viral world. Second, bacterial host strains were needed for the investigation of bacteriophages. Third, besides the classic overlay plaque assay for isolation, the dsDNA, ssDNA, dsRNA, and ssRNA was also isolated from phage plaques as well.To accomplish this endeavor, a local bacterial host system associated with various RNA and DNA viruses was required. Such hosts were not available at the beginning of this work and making it necessary to isolate a suitable prokaryotic system. To this end, environmental samples were successfully screened for new hosts, resulting in 37 new candidate bacterial strains, eight of which were sequenced and genomically analyzed (Brevundimonas pondensis, B. goettingensis, Serratia marcescens LVF3, Luteibacter flocculans, Stenotrophomonas indicatrix DAIF1, Kinneretia sp. DAIF2, and Janthinobacterium lividum EIF1 and EIF2). These were evaluated for their suitability as host systems (chapter 3.1 to 3.5, 3.8 and 3.9). A total of four new species were discovered and described. Using genomic analyses three of these were fully characterized (Brevundimonas pondensis, Brevundimonas goettingensis, and Luteibacter flocculans).Brevundimonas pondensis LVF1 and Serratia marcescens LVF3 proved to be particularly promising candidates to achieve the main objectives of this thesis (chapter 3.7). They were used for classical phage isolation, resulting in 25 new dsDNA phages: 14 were associated with Brevundimonas and 11 with Serratia. TEM analysis revealed that six are myoviruses, 18 siphoviruses and one podovirus, while the Brevundimonas-associated phages are all Ines Friedrich Summary• 2 • siphoviruses. The classical approach was complemented by Next Generation Sequencing (NGS)-based methods that provided dsDNA, ssDNA, dsRNA, and ssRNA host-associated virome data. Furthermore, the complementary NGS approach enabled the identification of vB_SmaP-Kaonashi and vB_SmaM-Otaku. The latter is a virus that infects both host systems.In addition, the ssDNA virome associated with Brevundimonas pondensis revealed promising results as two contigs associated with ssDNA phages could be detected. These belong to the family Microviridae and Inoviridae. Further, the ssRNA virome of Brevundimonas goettingensis contained a contig associated with the Caulobacter-associated RNA phage phiCb5 which belong to the family Leviviridae.In addition, bacteriophage isolates associated with the bacterial host strains Janthinobacterium (chapter 3.8) and Luteibacter (chapter 3.9) were found. Here, using the classical phage isolation approach, one phage was discovered for each bacterial host system.Janthino...

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“…and Brachymonas sp. are known to poorly grow on propionate ( Hiraishi, 2015 , Vancanneyt et al., 2015 ), which could explain why Brevundimonas sp. were washed out over time in our experiments, e.g.…”

Section: Discussionmentioning

confidence: 99%